| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-32026-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31974-25ul | |||
HAF016). A specific band for PLC-beta 3 was detected at approx-imately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows recombinant human PLC-beta 1, PLC-beta 2, PLC-beta 3, PLC-beta 4 (5 ng/lane). PVDF mem-brane was probed with 1 µg/mL Sheep Anti-Human PLC-beta 3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4716) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for PLC-beta 3 was detected at approx-imately 29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4716-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-93347-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22249-50ul | |||
![Western Blot: PLC-beta 3 Antibody [NBP2-32026] - Analysis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar pattern to independent antibody NBP2-31974 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-32026-img0009.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PLC-beta 3 Antibody [NBP2-32026] - Analysis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar pattern to independent antibody NBP2-31974 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PLC-beta 3 Antibody [NBP2-32026] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-32026-img0007.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human duodenum.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0012.jpg)
![Immunohistochemistry: PLC-beta 3 Antibody [NBP2-32026] - Staining of human small intestine shows moderate cytoplasmic and luminal membranous positivity in glandular cells.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-NBP2-32026-img0005.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human cerebral cortex, duodenum, lymph node and testis using Anti-PLCB3 antibody NBP2-32026 (A) shows similar protein distribution across tissues to independent antibody NBP2-31974 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0008.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human cerebral cortex.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0011.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-32026] - Staining of human testis.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-32026-img0013.jpg)
![Western Blot: PLC-beta 3 Antibody [NBP2-31974] - Analysis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar pattern to independent antibody NBP2-32026 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-31974-img0015.jpg "<b>Independent Antibodies Validation. </b>Western Blot: PLC-beta 3 Antibody [NBP2-31974] - Analysis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar pattern to independent antibody NBP2-32026 (B).")
![<b>Independent Antibodies Validation. </b>Immunocytochemistry/Immunofluorescence: PLC-beta 3 Antibody [NBP2-31974] - Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-31974-img0010.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human testis.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0018.jpg)
![Immunohistochemistry: PLC-beta 3 Antibody [NBP2-31974] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-NBP2-31974-img0008.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0011.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human small intestine shows high expression.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0012.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining in human small intestine and cerebral cortex tissues using anti-PLCB3 antibody. Corresponding PLCB3 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0013.jpg)
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human cerebral cortex, duodenum, lymph node and testis using Anti-PLCB3 antibody NBP2-31974 (A) shows similar protein distribution across tissues to independent antibody NBP2-32026 (B).](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0014.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human duodenum.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0016.jpg)
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-31974] - Staining of human lymph node.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-31974-img0017.jpg)
HAF016). A specific band for PLC-beta 3 was detected at approximately 150 kDa (as indicated). This experiment was conducted under reducing conditions and using ![Western Blot: PLC-beta 3 Antibody [NBP2-93347] - Analysis of extracts of various cell lines, using PLC-beta 3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-93347-img0001.jpg "Western Blot: PLC-beta 3 Antibody [NBP2-93347] - Analysis of extracts of various cell lines, using PLC-beta 3 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 30s.")
![Immunohistochemistry-Paraffin: PLC-beta 3 Antibody [NBP2-93347] - Immunohistochemistry of paraffin-embedded rat brain using PLC-beta 3 Rabbit pAb (NBP2-93347) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Immunohistochemistry-Paraffin-NBP2-93347-img0002.jpg)
![Immunohistochemistry: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32776) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32776_rabbit-plc-beta-3-mab-je39-14-298202414262641.jpg "Immunohistochemistry: PLC-beta 3 Antibody (JE39-14) [NBP3-32776] - Immunohistochemical analysis of paraffin-embedded human small intestine tissue with Rabbit anti-PLC-beta 3 antibody (NBP3-32776) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32776) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western blot analysis on different lysates with NBP3-32776 at 1/1,000 dilution. Lane 1: K-562 cell lysate (20 µg/Lane) Lane 2: MCF7 cell lysate (20 µg/Lane) Lane 3: HeLa cell lysate (20 µg/Lane) Lane 4: HCT 116 cell lysate (20 µg/Lane) Lane 5: SH-SY5Y cell lysate (20 µg/Lane) Predicted band size: 139 kDa Observed band size: 150 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32776 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG [HRP] Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32776_-western-blot-639204595237303911.jpg)
![Western Blot: PLC-beta 3 Antibody (SR1198) [NBP3-22249] - Western blot analysis using NBP3-22249 in A431 cell lysate.](http://images.novusbio.com/fullsize/nbp3-22249_rabbit-plc-beta-3-mab-sr1198-57202315545136.jpg "Western Blot: PLC-beta 3 Antibody (SR1198) [NBP3-22249] - Western blot analysis using NBP3-22249 in A431 cell lysate.")
![Immunoprecipitation:PLC-beta 3 AntibodyNBP3-30092] -Detection of human PLCB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLCB3 antibody NBP3-30092 (lot NBP3-30092-1) used for IP at 6 µg per reaction. PLCB3 was also immunoprecipitated by rabbit anti-PLCB3 antibody. For blotting immunoprecipitated PLCB3, NBP3-30092 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30092_rabbit-plc-beta-3-pab-301220241310101.jpg "Immunoprecipitation:PLC-beta 3 AntibodyNBP3-30092] -Detection of human PLCB3 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PLCB3 antibody NBP3-30092 (lot NBP3-30092-1) used for IP at 6 µg per reaction. PLCB3 was also immunoprecipitated by rabbit anti-PLCB3 antibody. For blotting immunoprecipitated PLCB3, NBP3-30092 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: PLC-beta 3 Antibody (4C0Z7) [NBP3-15885] - Western blot analysis of extracts of various cell lines, using PLC beta 3 (PLC-beta 3) Rabbit mAb (NBP3-15885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-4C0Z7-Western-Blot-NBP3-15885-img0001.jpg "Western Blot: PLC-beta 3 Antibody (4C0Z7) [NBP3-15885] - Western blot analysis of extracts of various cell lines, using PLC beta 3 (PLC-beta 3) Rabbit mAb (NBP3-15885) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western Blot: PLC-beta 3 Antibody [NBP2-88061] - Host: Rabbit. Target Name: PLCB3. Sample Tissue: Human Jurkat Whole Cell lysates. Antibody Dilution: 1ug/ml](http://images.novusbio.com/fullsize/PLC-beta-3-Antibody-Western-Blot-NBP2-88061-img0001.jpg "Western Blot: PLC-beta 3 Antibody [NBP2-88061] - Host: Rabbit. Target Name: PLCB3. Sample Tissue: Human Jurkat Whole Cell lysates. Antibody Dilution: 1ug/ml")
