HAF016). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF016). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, H4-II-E-C3 rat hepatoma cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7568) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counter-stained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutralized (green line) by increasing concentrations of Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_lightbox"> 229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutralized (green line) by increasing concentrations of Human PD-ECGF/Thymidine Phosphorylase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1143). The ND₅₀ is typically 0.5-2.0 µg/mL." class="big_thumb" />

HAF005). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF005). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of A431 human epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Monoclonal Antibody (Catalog # MAB7568) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND₅₀ is typically 10-25 µg/mL." class="big_lightbox"> 229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND₅₀ is typically 10-25 µg/mL." alt="Recombinant Human PD-ECGF/Thymidine Phosphorylase (Catalog # 229-PE) stimulates proliferation in HUVEC human umbilical vein endothelial cells in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human PD-ECGF/Thymidine Phosphorylase (150 ng/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Human PD-ECGF/Thymidine Phosphorylase Polyclonal Antibody (Catalog # AB-229-NA). The ND₅₀ is typically 10-25 µg/mL." class="big_thumb" />