AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox"> AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." alt="Mouse splenocytes were stained with Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />

555-TS) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse IL-7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_lightbox"> 555-TS) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with mouse IL-7 Ra/CD127 in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse TSLP (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF546). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_thumb" />

HAF017). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF017). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line either mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF019). A specific band was detected for TSLP R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_lightbox"> HAF019). A specific band was detected for TSLP R at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />

CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox"> CTS008) and counter-stained with hematoxylin (blue). Specific staining was localized to the plasma membrane. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and cell surface. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.3 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 1398-TS) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-7 Ra and TSLP R in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human TSLP (0.3 ng/mL) is neutralized (green line) by increasing concen-trations of Goat Anti-Human TSLP R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF981). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0102B). View our protocol for Staining Membrane-associated Proteins. " alt="HEK cells transfected with hTSLPR (filled histogram) vs Irrelevant HEK transfectant cells (open histogram) were stained with Mouse Anti-Human TSLPR Monoclonal Antibody (Catalog # MAB981), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

HAF018). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF018). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of NS0 mouse myeloma cell line mock transfected or transfected with human TSLP R. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human TSLP R Monoclonal Antibody (Catalog # MAB9811) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). Specific bands were detected for TSLP R at approximately 45-60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " alt="NS0/hTSLPR cells were stained with Mouse Anti-Human TSLPR Monoclonal Antibody (Catalog # MAB981R, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox"> F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

1398-TS) is immobilized at 0.5 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) with an ED₅₀ of 0.75-7.5 ng/mL." class="big_lightbox"> 1398-TS) is immobilized at 0.5 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) with an ED₅₀ of 0.75-7.5 ng/mL." alt="When Human TSLP Protein (Catalog # 1398-TS) is immobilized at 0.5 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human TSLPR Fc Chimera Avi-tag (Catalog # AVI981) with an ED₅₀ of 0.75-7.5 ng/mL." class="big_thumb" />