| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-91189SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21219-25ul | |||
HAF019). A specific band was detected for MBD4 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MBD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5935) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MBD4 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5935-SP | |||
![Immunocytochemistry/Immunofluorescence: MBD4 Antibody [NBP1-91189] - MBD4 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/MBD4-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-91189-img0003.jpg "Immunocytochemistry/Immunofluorescence: MBD4 Antibody [NBP1-91189] - MBD4 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).")
![Immunohistochemistry: MBD4 Antibody [NBP1-91189] - IHC analysis of MBD4 in human breast cancer cells using DAB with hematoxylin counterstain.](http://images.novusbio.com/fullsize/MBD4-Antibody-Immunohistochemistry-NBP1-91189-img0004.jpg)
![Western Blot: MBD4 Antibody [NBP2-24393] - analysis of MBD4 in Jurkat cell lysate (30ug) using anti-MBD4 antibody followed by goat anti-rabbit HRP conjugated secondary antibody (Cat. #NB7160). Image from verified customer review.](http://images.novusbio.com/fullsize/MBD4-Antibody-Western-Blot-NBP2-24393-img0002.jpg "Western Blot: MBD4 Antibody [NBP2-24393] - analysis of MBD4 in Jurkat cell lysate (30ug) using anti-MBD4 antibody followed by goat anti-rabbit HRP conjugated secondary antibody (Cat. #NB7160). Image from verified customer review.")
![Western Blot: MBD4 Antibody [NBP2-24393] - Analysis of MBD4 in HL60 cell lysate probed with MBD4 antibody.](http://images.novusbio.com/fullsize/MBD4-Antibody-Western-Blot-NBP2-24393-img0001.jpg)
![Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human cell line A-431Lane 5: Human liver tissue](http://images.novusbio.com/fullsize/nbp3-21219_rabbit-mbd4-pab-45202315493245.jpg)
![Immunohistochemistry:[NBP3-30657]-Detection of human MBD4 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MBD4 used at a dilution of 1: 200. Detection: DAB](http://images.novusbio.com/fullsize/nbp3-30657_rabbit-mbd4-pab-23120251158551.jpg "Immunohistochemistry:[NBP3-30657]-Detection of human MBD4 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MBD4 used at a dilution of 1: 200. Detection: DAB")
![Western Blot: MBD4 Antibody [NBP3-18657] - Figure 2. Western blot analysis using the antibody directed against MBD4 Figure 2A: Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MBD4 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. Figure 2B: Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 ug) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.](http://images.novusbio.com/fullsize/MBD4-Antibody-Western-Blot-NBP3-18657-img0001.jpg "Western Blot: MBD4 Antibody [NBP3-18657] - Figure 2. Western blot analysis using the antibody directed against MBD4 Figure 2A: Human osteosarcoma cells (U2OS) were transfected with an expression vector for TY1-tagged MBD4. The presence of TY1-MBD4 in the cell lysates was demonstrated by western blot analysis with the antibody against the TY1-tag (lane 1) and with the antibody against MBD4 (lane 2), diluted 1:2,000 in TBST containing 3% milk powder. Figure 2B: Western blot was performed on nuclear extracts from the U937 human leukemic monocyte lymphoma cell line (40 ug) with the antibody against MBD4, diluted 1:2,000 in TBST containing 3% milk powder. A molecular weight marker (in kDa) is shown on the left. The location of the protein of interest is indicated on the right.")
![ELISA: MBD4 Antibody [NBP3-18657] - Figure 1. Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human MBD4 in antigen coated wells. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:15,000.](http://images.novusbio.com/fullsize/MBD4 Antibody-ELISA-NBP3-18657-img0002.jpg)
![Immunohistochemistry:[NBP3-29208]-Detection of human MBD4 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-MBD4.](http://images.novusbio.com/fullsize/nbp3-29208_rabbit-mbd4-pab-231202512101623.jpg "Immunohistochemistry:[NBP3-29208]-Detection of human MBD4 by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-MBD4.")
![Western Blot: MBD4 Antibody [NBP3-10031] - Western blot analysis of MBD4 in Human ACHN Whole Cell lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/MBD4-Antibody-Western-Blot-NBP3-10031-img0001.jpg "Western Blot: MBD4 Antibody [NBP3-10031] - Western blot analysis of MBD4 in Human ACHN Whole Cell lysates. Antibody dilution at 1ug/ml")
![Chromatin Immunoprecipitation: MBD4 Antibody [NBP3-10344] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.](http://images.novusbio.com/fullsize/MBD4-Antibody-Chromatin-Immunoprecipitation-NBP3-10344-img0001.jpg "<b>Biological Strategies Validation. </b>Chromatin Immunoprecipitation: MBD4 Antibody [NBP3-10344] - Quiescent human colon carcinoma HCT116 cultures were treated with 10% FBS for three time points (0, 15, 30min) or (0, 30, 60min) were used in Matrix-ChIP and real-time PCR assays at EGR1 gene (Exon1) and 15kb upstream site.")
