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HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to sinusoids. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox" target="_blank">
AB-105-C, open histogram), followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox" target="_blank">
HAF008). A specific band was detected for Lactate Dehydrogenase B/LDHB at approximately 36 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox" target="_blank">
Lactate dehydrogenase (LDH) is an ubiquitous enzyme commonly found in wide variety of organisms, including plants and microbes. LDH is involved in the interconversion of the pyruvate and NADH to lactate and NAD+. It is also called Hydroxybutyrate Dehydrogenase (HBD), because it can catalyze the oxidation of hydroxybutyrate (1). In mammals, three types of LDH subunits (35 kDa) are encoded by the genes Ldh-A, Ldh-B, and Ldh-C. All LDH subunits can combine to form various terameric isoenzymes (140 kDa). Lactate dehydrogenase B (LDH-B, heart subunit, LDH-H) is involved in the conversion of L-lactate and NAD to pryruvate and NADH and it is predominantly localized in the heart tissue. Similar to other LDH subunit, LDH-B is considered to be an important marker for germ cell tumor (2).
![Western Blot Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]](http://images.novusbio.com/images/antibody/Lactate_Dehydrogenase_B_MAB9205_Western_Blot_20480.jpg)
![Simple Western Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]](http://images.novusbio.com/fullsize/mab9205_human-lactate-dehydrogenase-b-ldhb-mab-clone-2057d-simple-western-2510202483235..jpg)
![Immunohistochemistry Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]](http://images.novusbio.com/images/antibody/Lactate_Dehydrogenase_B_MAB9205_Immunohistochemistry_20491.jpg)
![Immunocytochemistry Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]](http://images.novusbio.com/images/antibody/Lactate_Dehydrogenase_B_MAB9205_Immunocytochemistry__Immunofluorescence_20490.jpg)
![Intracellular Staining by Flow Cytometry Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]](http://images.novusbio.com/images/antibody/Lactate_Dehydrogenase_B_MAB9205_Flow_Cytometry_20481.jpg)
![Knockout Validated Lactate Dehydrogenase B Antibody (2057D) [Unconjugated]](http://images.novusbio.com/images/antibody/Lactate_Dehydrogenase_B_MAB9205_Knockout_Validated_21945.jpg)
