| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB400-106SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5109-SP | |||
CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="PLTP was detected in perfusion fixed frozen sections of mouse brain (medulla) using Sheep Anti-Mouse PLTP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4918) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4918-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 4918-PL-025/CF | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-22273-50ul | |||
![Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0006.jpg "Western Blot: PLTP Antibody [NB400-106] - Activation of PLTP in STAT3 pathway. RA-FLS were stimulated for 24 hours with rhPLTP at the indicated concentrations. Western blot analyses analyzed cell lysates for phosphorylation of STAT3 (Tyr705). Band intensities were normalized to corresponding STAT3 band intensities. Representative Western blots are shown (n = 3).Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.")
![Immunocytochemistry/Immunofluorescence: PLTP Antibody [NB400-106] - PLTP antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunocytochemistry-Immunofluorescence-NB400-106-img0002.jpg)
![Western Blot: PLTP Antibody [NB400-106] - WB analysis of PLTP in human brain lysate.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NB400-106-img0001.jpg)
![<b>Biological Strategies Validation. </b>Immunohistochemistry: PLTP Antibody [NB400-106] - Recombinant PLTP antibody induced cytokine production and FLS proliferation, independent of its lipid transfer ability. (A) RA-FLS were stimulated for 48 hours with PLTP or heat inactivated-PLTP and proliferation was evaluated during the final day of stimulation using thymidine incorporation. (B) Blockade of PLTP decreased the effect of rhPLTP on FLS proliferation. (C) PLTP impact on RA-FLS cytokine production. (D) OA-FLS were treated with either native PLTP or heated-PLTP and analyzed for cytokine production and proliferation. Citation: Audo R, Deckert V, Daien CI, Che H, Elhmioui J, Lemaire S, et al. (2018) PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity. PLoS ONE 13(3): e0193815.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-NB400-106-img0005.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody [NB400-106] - PLTP was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human PLTP polyclonal Antibody (Catalog # NB400-106) at 1:300 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in decidual cells.](http://images.novusbio.com/fullsize/PLTP-Antibody-Immunohistochemistry-Paraffin-NB400-106-img0007.jpg)
![Immunohistochemistry: PLTP Antibody [NB400-106] - PLTP expression in RA synovial tissues.For immuno-histological analysis, (A) synovial tissue sections from RA patients (n = 5) were stained for PLTP or macrophages (CD68+ cells). Representative images obtained for immunohistological staining are shown. Blue arrows show macrophages (non-exhaustive), determined as CD68+ cells. FLS are determined with morphological features & CD68+ staining (black arrow; non-exhaustive). (B, C) Double staining was performed to visualize localization (n = 3) of PLTP with macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C). Fluorescence was analyzed at 20x magnification. Overlay is shown to visualize co-localization of PLTP in macrophages (CD68+) (B) or RA-FLS ( alpha -SMA+) (C) or PLTP expression in infiltrate. Original magnification: 20x. Separate images can be found in S1 Fig. Image collected & cropped by CiteAb from the following publication (//dx.plos.org/10.1371/journal.pone.0193815), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nb400-106_rabbit-polyclonal-pltp-antibody-310202416171467.jpg)
![Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of extracts of various cell lines, using PLTP antibody (NBP3-16847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Western-Blot-NBP3-16847-img0003.jpg "Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of extracts of various cell lines, using PLTP antibody (NBP3-16847) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.")
![Immunohistochemistry-Paraffin: PLTP Antibody (6A1Q1) [NBP3-16847] - Immunohistochemistry of paraffin-embedded mouse testis using PLTP Rabbit mAb (NBP3-16847) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Immunohistochemistry-Paraffin-NBP3-16847-img0002.jpg)
![Immunohistochemistry-Paraffin: PLTP Antibody (6A1Q1) [NBP3-16847] - Immunohistochemistry of paraffin-embedded rat pancreas using PLTP Rabbit mAb (NBP3-16847) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.](http://images.novusbio.com/fullsize/PLTP-Antibody-6A1Q1-Immunohistochemistry-Paraffin-NBP3-16847-img0001.jpg)
![Western Blot: PLTP Antibody (6A1Q1) [NBP3-16847] - Western blot analysis of various lysates using PLTP Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.](http://images.novusbio.com/fullsize/nbp3-16847_rabbit-pltp-mab-6a1q1-52202511361237.jpg)
![Western Blot: PLTP Antibody [H00005360-D01P] - Analysis of PLTP expression in mouse liver.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-H00005360-D01P-img0003.jpg "Western Blot: PLTP Antibody [H00005360-D01P] - Analysis of PLTP expression in mouse liver.")
![Western Blot: PLTP Antibody [H00005360-D01P] - Analysis of PLTP expression in transfected 293T cell line by PLTP polyclonal antibody.Lane 1: PLTP transfected lysate(54.70 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-H00005360-D01P-img0004.jpg)
![ELISA: Human PLTP ELISA Kit (Colorimetric) [NBP3-00415] - Samples were spiked with high concentrations of Human PLTP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-00415_human-pltp-elisa-kit-colorimetric-132202416184875.jpg "ELISA: Human PLTP ELISA Kit (Colorimetric) [NBP3-00415] - Samples were spiked with high concentrations of Human PLTP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human PLTP ELISA Kit (Colorimetric) [NBP3-00415] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-00415_human-pltp-elisa-kit-colorimetric-432024941538.jpg)
![Western Blot: PLTP Antibody (SR2240) [NBP3-22273] - Western blot analysis using NBP3-22273 in HeLa cell lysate.](http://images.novusbio.com/fullsize/nbp3-22273_rabbit-pltp-mab-sr2240-57202317215118.jpg "Western Blot: PLTP Antibody (SR2240) [NBP3-22273] - Western blot analysis using NBP3-22273 in HeLa cell lysate.")
![Western Blot: PLTP Overexpression Lysate (Adult Normal) [NBL1-14528] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLTP.](http://images.novusbio.com/fullsize/PLTP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-14528-img0002.jpg "Western Blot: PLTP Overexpression Lysate (Adult Normal) [NBL1-14528] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLTP.")
![Western Blot: PLTP Overexpression Lysate (Adult Normal) [NBP2-04830] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLTP.](http://images.novusbio.com/fullsize/PLTP-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04830-img0001.jpg "Western Blot: PLTP Overexpression Lysate (Adult Normal) [NBP2-04830] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PLTP.")
![Western Blot: PLTP Antibody [NBP3-10028] - Western blot analysis of PLTP in Human Jurkat Whole Cell lysates. Antibody dilution at 1ug/ml](http://images.novusbio.com/fullsize/PLTP-Antibody-Western-Blot-NBP3-10028-img0001.jpg "Western Blot: PLTP Antibody [NBP3-10028] - Western blot analysis of PLTP in Human Jurkat Whole Cell lysates. Antibody dilution at 1ug/ml")
