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src Products By Gene Name

23 Results for "src"
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AF2685

Src [p Tyr419] Antibody [Unconjugated]

(1 Review)
(6 Publications)
100 μg
AF2685
Western blot shows lysates of MDA‑MB‑468 human breast cancer cell line and HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 50 μM pervanadate (PV) for 10 minutes. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y419) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2685), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-Src (Y419) at approximately 56 - 61 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, IHC
Applications: WB, Simple Western, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF2685-SP
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AF3389

Src Antibody [Unconjugated]

(4 Publications)
100 μg
AF3389
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HL‑60 human acute promyelocytic leukemia cell line (negative control). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Src Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3389) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Src at approximately 60 kDa (as indicated). GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, IHC
Applications: WB, Simple Western, IHC
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF3389-SP
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NBP1-19188

Src Antibody (5A18)

(2 Reviews)
(2 Publications)
0.05 mg
NBP1-19188
Western Blot: Src Antibody (5A18) [NBP1-19188] - Human breast cancer cell line MDA-MB-231 was treated with carboplatin for 72 hours and the expression of p-Src at Y419 and total Src were detected by western blot. From a verified customer review.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, IHC, IHC-P
Applications: WB, Simple Western, IHC, IHC-P
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
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MAB2685

Src [p Tyr416] Antibody (1246F) [Unconjugated]

(2 Publications)
100 μg
MAB2685
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line untreated (-) or treated (+) with 1 mM Pervanadate (PV) for 5 minutes, loaded at 0.2 mg/mL. A specific band was detected for Src at approximately 62 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human Phospho-Src (Y416) Monoclonal Antibody (Catalog # MAB2685) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Hu
Species Glossary
Reactivity: Hu
WB, Simple Western, ICC/IF
Applications: WB, Simple Western, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2685-SP
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MAB3389

Src Antibody (327537) [Unconjugated]

(2 Publications)
100 μg
MAB3389
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HL‑60 human acute promyelocytic leukemia cell line (negative control). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Src Monoclonal Antibody (Catalog # MAB3389) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Src at approximately 60 kDa (as indicated). GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB3389-SP
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DYC2685-2

Human Phospho-Src (Y419) DuoSet IC ELISA, 2 Plate

(2 Publications)
1 Kit (for 2 Plates)
DYC2685-2
A lysate prepared from MDA-MB-468 human breast cancer cells treated with 50 mM sodium pervanadate for 20 minutes was analyzed with this ELISA. The Human Phospho-Src (Y419) Detection Antibody was either untreated (no peptide) or preincubated with a phospho-peptide containing the phospho-Src Y419 phosphorylation site, a phospho-peptide containing the Ret Y905 phosphorylation site, a phospho-peptide containing the Tie-2 Y1100 phosphorylation site, or a phospho-peptide containing the PP2A Y307 phosphorylation site. Peptides were used at 40 ng/mL. Only the phospho-peptide containing the Src Y419 phosphorylation site blocked the signal, indicating that the ELISA is specific for human Src phosphorylation.
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC2685-5
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MAB3807

Src Antibody (327554) [Unconjugated]

100 μg
MAB3807
Western blot shows lysates of A549 human lung carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, U937 human histiocytic lymphoma cell line (negative control), and HL‑60 human acute promyelocytic leukemia cell line (negative control). PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Src Monoclonal Antibody (Catalog # MAB3807) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Src at approximately 60 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Hu
Species Glossary
Reactivity: Hu
WB, IHC
Applications: WB, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB3807-SP
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NBP3-15675

Src Antibody (7G6M9)

100 ul
NBP3-15675
Western Blot: Src Antibody (7G6M9) [NBP3-15675] - Western blot analysis of extracts of Jurkat cells, using Src antibody (NBP3-15675) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IP, KO
Applications: WB, ICC/IF, IP, KO
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
         
Validated by:          
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H00006714-M01

Src Antibody (1B9)

0.1 mg
H00006714-M01
Immunocytochemistry/Immunofluorescence: Src Antibody (1B9) [H00006714-M01] - Analysis of monoclonal antibody to SRC on HeLa cell . Antibody concentration 10 ug/ml.
Hu
Species Glossary
Reactivity: Hu
WB, ELISA, ICC/IF, IHC, IHC-P, PLA
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, PLA
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
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NBP3-13245

Src [p Tyr527] Antibody

100 ul
NBP3-13245
Western Blot: Src [p Tyr527] Antibody [NBP3-13245] - Untreated (-) and treated (+) NIH-3T3 whole cell extracts (20 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Src (phospho Tyr527) antibody (NBP3-13245) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Hu, Mu, Rt, Bv, Ch, RM, Ze
Species Glossary
Reactivity: Hu, Mu, Rt, Bv, Ch, RM, Ze
WB
Applications: WB
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
         
Validated by:          
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NBP3-05396

Src [p Tyr529] Antibody

100 ul
NBP3-05396
Western Blot: Src [p Tyr529] Antibody [NBP3-05396] - Western blot analysis of extracts of various cell lines, using Src pAb (NBP3-05396) at 1:1000 dilution.Hela cells were treated by ATP(5 mM) at 30C for 1 hour or treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC, IHC-P
Applications: WB, ICC/IF, IHC, IHC-P
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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NBP3-20055

Src Antibody (S05-7H8)

100 ul
NBP3-20055
Western Blot: Src Antibody (S05-7H8) [NBP3-20055] - Western blot detection of Src in C6, 3T3 cell lysates using NBP3-20055 (1:1000 diluted). Predicted band size: 60kDa. Observed band size: 60kDa.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC, IHC-P
Applications: WB, IHC, IHC-P
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
compare
(select up to 3 products from this page to compare)
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