| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71988 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-22832SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89949-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89950-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-77330-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-71988 | |||
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a and recruits CPC components. Lysates of TN-arrested Aurora B-depleted cells were supplemented with recombinant Survivin and Borealin proteins with or without recombinant Aurora B protein. The lysates were incubated with antibodies against INCENP and the H3R2me2a peptide, and pulldown was conducted with agarose A beads. The binding was visualized by immunoblotting. The asterisk denotes the light chain of antibodies. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14511-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-89951-img0018.jpg "Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a and recruits CPC components. Lysates of TN-arrested Aurora B-depleted cells were supplemented with recombinant Survivin and Borealin proteins with or without recombinant Aurora B protein. The lysates were incubated with antibodies against INCENP and the H3R2me2a peptide, and pulldown was conducted with agarose A beads. The binding was visualized by immunoblotting. The asterisk denotes the light chain of antibodies. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14511-w), licensed under a CC-BY license.")
![Immunocytochemistry/Immunofluorescence: Borealin Antibody [NBP1-89951] - Staining of human cell line U-251 MG shows localization to nucleus & nucleoli. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89951-img0010.jpg)
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining in human testis and skeletal muscle tissues using NBP1-89951 antibody. Corresponding CDCA8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0016.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Analysis in control (vector only transfected HEK293T lysate) and CDCA8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-89951-img0011.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Borealin Antibody [NBP1-89951] - H3R2me2a acts as a histone mark for CPC recruitment on chromosome arms. The cytosol and chromosome fractions were isolated by centrifugation from mitotic HeLa cells treated with Haspin or a PRMT6 inhibitor and analyzed by Western blot probing for the indicated proteins. Histone H3 and tubulin served as loading controls for chromosome and cytosol fractions, respectively. Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41467-020-14511-w), licensed under a CC-BY license.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-89951-img0017.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human duodenum shows strong nuclear positivity in a subset of glandular cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0012.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human lymphoid tissues shows moderate nuclear positivity in germinal center cells.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0013.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0014.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89951-img0015.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416113528.jpg)
![Western Blot: Borealin Antibody [NBP1-89951] - Aurora B preferentially binds to H3R2me2a & recruits CPC components.a The amino acid sequences of modified histone H3 peptides. b In vitro Haspin kinase assay w/ 21-aa peptide. c In vitro Aurora B kinase assay w/ 21-aa peptide for phosphorylation. d In vitro PRMT6 methyltransferase assay. e–h The cells treated w/ siPRMT6, PRMT6 inhibitor MS023, or the Haspin kinase inhibitor CHR-6494. The intensities of H3R2me2a (f) & H3T3ph (h) in chromosome arm & centromere analyzed by IF microscopy of nocodazole-arrested HeLa chromosome spreads & plotted (n = 100 chromosomes from three independent experiments). i After transfection of indicated plasmids, HeLa cells treated w/ a Haspin kinase inhibitor, CHR-6494, for 5 h & the intensity of CPC components analyzed for 100 centromeres from three independent experiments. j Recombinant CPC proteins incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. k Lysates of TN-arrested Aurora B-depleted cells supplemented w/ recombinant Survivin & Borealin proteins w/ or w/out recombinant Aurora B protein. The lysates incubated w/ antibodies against INCENP & the H3R2me2a peptide, & pulldown conducted w/ agarose A beads. The binding visualized by immunoblotting. The asterisk denotes the light chain of antibodies. l Lysates of TN-arrested mitotic HeLa cells incubated w/ biotinylated histone peptides & pulled down w/ streptavidin-agarose beads, & the binding visualized by immunoblotting. Relative band intensities of band measured w/ image processing software (Image Studio ver5.0). Error bars, SEMs. Scale bars, 5 μm. Source data are provided as a Source Data file. (Student’s t-test *p < 0.01). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32001712), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89951_rabbit-polyclonal-borealin-antibody-310202416121943.jpg)
![Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Western-Blot-NBP2-46199-img0006.jpg "Western Blot: Borealin Antibody (4G3) [NBP2-46199] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Borealin.")
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human tonsil tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0005.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0001.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Carcinoma of Human lung tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0002.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0003.jpg)
![Immunohistochemistry: Borealin Antibody (4G3) [NBP2-46199] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)](http://images.novusbio.com/fullsize/Borealin-Antibody-4G3-Immunohistochemistry-NBP2-46199-img0004.jpg)
![Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-22832-img0004.jpg "Western Blot: Borealin Antibody [NBP1-22832] - Mouse kidney lysate.")
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89949] - Staining in human testis and kidney tissues using anti-CDCA8 antibody. Corresponding CDCA8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89949-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89949] - Staining in human testis and kidney tissues using anti-CDCA8 antibody. Corresponding CDCA8 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Borealin Antibody [NBP1-89949] - Analysis in control (vector only transfected HEK293T lysate) and CDCA8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-89949-img0009.jpg)
![Western Blot: Borealin Antibody [NBP1-89949] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10. Lane 2: U-251 MG](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-89949-img0003.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89949] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89949-img0006.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89949] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89949-img0007.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89950] - Staining in human testis and endometrium tissues using anti-CDCA8 antibody. Corresponding CDCA8 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89950-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89950] - Staining in human testis and endometrium tissues using anti-CDCA8 antibody. Corresponding CDCA8 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: Borealin Antibody [NBP1-89950] - Analysis in control (vector only transfected HEK293T lysate) and CDCA8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-89950-img0009.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89950] - Staining of human endometrium shows low expression as expected.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89950-img0006.jpg)
![Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89950] - Staining of human testis shows high expression.](http://images.novusbio.com/fullsize/Borealin-Antibody-Immunohistochemistry-Paraffin-NBP1-89950-img0007.jpg)
![Western Blot: Borealin Antibody [NBP1-77330] - Rat kidney lysate with Borealin antibody at (A) 1 and (B) 2 ug/ml.](http://images.novusbio.com/fullsize/Borealin-Antibody-Western-Blot-NBP1-77330-img0005.jpg "Western Blot: Borealin Antibody [NBP1-77330] - Rat kidney lysate with Borealin antibody at (A) 1 and (B) 2 ug/ml.")
![Immunocytochemistry/ Immunofluorescence: Borealin Antibody - BSA Free [NBP1-77330] - Immunofluorescence of Borealin in Rat Kidney cells with Borealin antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/nbp1-77330_rabbit-polyclonal-borealin-antibody-2711202419353815.jpg)