| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF2038-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12075-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48393 | |||
| Azide and BSA Free | NBP3-11562 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-33746-25ul | |||
![Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Western-Blot-NBP3-12075-img0001.jpg "Western Blot: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Daudi(A) cell lysate, K562(B) cell lysate and human bone marrow(C) tissue lysate (35ug protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 84-3C1f (NBP3-12075) at 0.1 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (84-3C1) - Chimeric [NBP3-12075] - Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 84-3C1 (NBP3-12075) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12075, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-84-3C1-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12075-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (84-3C1) - Chimeric - Azide and BSA Free [NBP3-12075] - K562 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12075 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12075_rabbit-monoclonal-cd43-sialophorin-antibody-84-3c1-chimeric-74202522141237.png)
![Western Blot: CD43/Sialophorin Antibody [NBP2-32115] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody NBP2-32115 used for IP at 6 ug per reaction. CD43 was also immunoprecipitated by rabbit anti-CD43 antibody BL16255. For blotting immunoprecipitated CD43,NBP2-32115 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Western-Blot-NBP2-32115-img0002.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CD43/Sialophorin Antibody [NBP2-32115] - Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using RIPA buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody NBP2-32115 used for IP at 6 ug per reaction. CD43 was also immunoprecipitated by rabbit anti-CD43 antibody BL16255. For blotting immunoprecipitated CD43,NBP2-32115 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry: CD43/Sialophorin Antibody [NBP2-32115] - Sample: FFPE section of human lung cancer infiltrating cells. Antibody: Affinity purified rabbit anti- CD43 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Immunohistochemistry-NBP2-32115-img0005.jpg)
![Western Blot: CD43/Sialophorin Antibody [NBP2-32115] - Whole cell lysate (50 ug) prepared using RIPA buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD43 antibody NBP2-32115 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Western-Blot-NBP2-32115-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (Bra7G) [NBP2-44650] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (Bra7G) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot .](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Bra7G-Immunocytochemistry-Immunofluorescence-NBP2-44650-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD43/Sialophorin Antibody (Bra7G) [NBP2-44650] - Immunofluorescence Analysis of K562 cells labeling CD43 with CD43/Sialophorin Antibody (Bra7G) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is NucSpot .")
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody (Bra7G) [NBP2-44650] - Human Spleen stained with CD43 Monoclonal Antibody (Bra7G).](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Bra7G-Immunohistochemistry-Paraffin-NBP2-44650-img0001.jpg)
![Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-278202415212446.jpg "Western Blot: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Western blot analysis of CD43/Sialophorin on different lysates with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: HL-60 cell lysate Lane 3: THP-1 cell lysate Lane 4: U-937 cell lysate Lane 5: HT-29 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 100-130 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32154) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunohistochemical analysis of paraffin-embedded human B lymphocyte tumor tissue with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32154) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-298202414161888.jpg)
![Immunocytochemistry/ Immunofluorescence: CD43/Sialophorin Antibody (A2F8-R) [NBP3-32154] - Immunocytochemistry analysis of U-937 (positive) and HT-29 (negative) labeling CD43/Sialophorin with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD43/Sialophorin antibody (NBP3-32154) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32154_mouse-cd43-sialophorin-mab-a2f8-r-2920241114826.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the FITC conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgM kappa Isotype Control FITC (left) or 0.06 ug of Anti-Mouse CD43 FITC (right).](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-R2-60-Flow-Cytometry-NBP1-43413-img0006.jpg "Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the FITC conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.06 ug of Rat IgM kappa Isotype Control FITC (left) or 0.06 ug of Anti-Mouse CD43 FITC (right).")
![Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Staining of C57Bl/6 splenocytes with 0.25 ug of Rat IgM Isotype Control Purified (open histogram) or 0.25 ug of Anti-Mouse CD43 Purified (filled histogram) followed by Anti-Rat IgM PE . Cells in the lymphocyte gate were used for analysis.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-R2-60-Flow-Cytometry-NBP1-43413-img0002.jpg)
![Flow Cytometry: CD43/Sialophorin Antibody (R2/60) [NBP1-43413] - Analysis using the Biotin conjugate of NBP1-43413. Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) APC and 0.25 ug of Rat IgM kappa Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse CD43 Biotin (right) followed by Streptavidin PE.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-R2-60-Flow-Cytometry-NBP1-43413-img0005.jpg)
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody [NBP2-33746] - Staining in human lymph node and cerebral cortex tissues using anti-SPN antibody. Corresponding SPN RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Immunohistochemistry-Paraffin-NBP2-33746-img0004.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody [NBP2-33746] - Staining in human lymph node and cerebral cortex tissues using anti-SPN antibody. Corresponding SPN RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody [NBP2-33746] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Immunohistochemistry-Paraffin-NBP2-33746-img0003.jpg)
![Immunohistochemistry-Paraffin: CD43/Sialophorin Antibody [NBP2-33746] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/CD43-Sialophorin-Antibody-Immunohistochemistry-Paraffin-NBP2-33746-img0002.jpg)
