| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56596SS | |||
| Azide and BSA Free | NBP2-33238 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4590-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-82450-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03713-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-76569-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-37533-0.025ml | |||
![Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody NB100-624 (lot NB100-624-2) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot NB100-624-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).](http://images.novusbio.com/fullsize/ROCK1-Antibody-NB100-624-img0003.jpg "Western Blot: ROCK1 Antibody [NB100-624] - Detection of human and mouse ROCK1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), HEK293T (T; 50 ug), Jurkat (J; 50 ug) and mouse NIH 3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ROCK1 antibody NB100-624 (lot NB100-624-2) used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ROCK1 was also immunoprecipitated by a previous lot (lot NB100-624-1) of this antibody. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 1 second (B).")
![Western Blot: cleaved ROCK1 Antibody (154C1465) [NB100-56596] - Analysis using Azide Free version of NB100-56596. Cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used](http://images.novusbio.com/fullsize/cleaved-ROCK1-Antibody-154C1465-Western-Blot-NB100-56596-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: cleaved ROCK1 Antibody (154C1465) [NB100-56596] - Analysis using Azide Free version of NB100-56596. Cleaved ROCK-1 in A) untreated, B) 1 hour and C) 4 hour staurosporin-treated (2 uM) Jurkat cells using this antibody. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used")
![Western Blot: ROCK1/2 Antibody (HL1632) - Azide and BSA Free [NBP3-25642] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (NBP3-25642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-2120249403897.jpg "Western Blot: ROCK1/2 Antibody (HL1632) - Azide and BSA Free [NBP3-25642] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (NBP3-25642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: ROCK1/2 Antibody (HL1632) - Azide and BSA Free [NBP3-25642] - Non-transfected (-) and transfected (+) 293T whole cell extract (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 + ROCK2 antibody [HL1632] (NBP3-25642) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-2120249403829.jpg)
![Immunohistochemistry-Paraffin: ROCK1/2 Antibody (HL1632) - Azide and BSA Free [NBP3-25642] - ROCK1 antibody [HL1632] detects ROCK1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. ROCK1 stained by ROCK1 antibody [HL1632] (NBP3-25642) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-212024940828.jpg)
![ROCK1/2 antibody [HL1632] detects ROCK1/2 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.ROCK1/2 stained by ROCK1/2 antibody [HL1632] (NBP3-25642) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-immunohistochemistry-paraffin-1411202518381724.jpg)
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1/2 antibody [HL1632] (NBP3-25642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-western-blot-1411202518312112.jpg)
![Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1/2 antibody [HL1632] (NBP3-25642) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-western-blot-1411202518405424.jpg)
![ROCK1/2 antibody [HL1632] detects ROCK1/2 protein at Golgi apparatus and cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ROCK1/2 stained by ROCK1/2 antibody [HL1632] (NBP3-25642) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-immunocytochemistry-immunofluorescence-141120251842528.jpg)
![ROCK1/2 antibody [HL1632] detects ROCK1/2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.ROCK1/2 stained by ROCK1/2 antibody [HL1632] (NBP3-25642) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25642_rabbit-rock1-2-mab-hl1632-azide-and-bsa-free-immunohistochemistry-paraffin-141120251832024.jpg)
![Western Blot: ROCK1 Antibody [NBP1-82450] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ROCK1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.](http://images.novusbio.com/fullsize/ROCK1-Antibody-Western-Blot-NBP1-82450-img0008.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ROCK1 Antibody [NBP1-82450] - Analysis in U-138MG cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ROCK1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: ROCK1 Antibody [NBP1-82450] - Staining of human colon shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/ROCK1-Antibody-Immunohistochemistry-Paraffin-NBP1-82450-img0005.jpg)
![Western Blot: ROCK1 Antibody [NBP1-82450] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/ROCK1-Antibody-Western-Blot-NBP1-82450-img0004.jpg)
![Western Blot: ROCK1 Antibody [NBP1-82450] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/ROCK1-Antibody-Western-Blot-NBP1-82450-img0009.jpg)
![Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464].](http://images.novusbio.com/fullsize/ROCK1-Antibody-464-Western-Blot-NBP2-42918-img0004.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464].")
![<b>Genetic Strategies Validation. </b>Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000](http://images.novusbio.com/fullsize/ROCK1-Antibody-464-Western-Blot-NBP2-42918-img0001.jpg)
![Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000](http://images.novusbio.com/fullsize/ROCK1-Antibody-464-Western-Blot-NBP2-42918-img0002.jpg)
![Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [464] dilution: 1:1000.](http://images.novusbio.com/fullsize/ROCK1-Antibody-464-Western-Blot-NBP2-42918-img0003.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT464].](http://images.novusbio.com/fullsize/ROCK1-Antibody-464-Western-Blot-NBP2-42918-img0005.jpg)
![Western Blot: ROCK1 Antibody (464) [NBP2-42918] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 (NBP2-42918) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-42918_mouse-monoclonal-rock1-antibody-464-91020242011426.jpg)
![Western Blot: ROCK1 Antibody (261) [NBP2-42917] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261].](http://images.novusbio.com/fullsize/ROCK1-Antibody-261-Western-Blot-NBP2-42917-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: ROCK1 Antibody (261) [NBP2-42917] - Untreated (-) and treated (+) PC-12 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261].")
![<b>Biological Strategies Validation. </b>Western Blot: ROCK1 Antibody (261) [NBP2-42917] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug HeLa whole cell lysate/extract C. 30 ug HepG2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [261] dilution: 1:1000](http://images.novusbio.com/fullsize/ROCK1-Antibody-261-Western-Blot-NBP2-42917-img0001.jpg)
![Western Blot: ROCK1 Antibody (261) [NBP2-42917] - Analysis of A. 30 ug Neuro2A whole cell lysate/extract B. 30 ug GL261 whole cell lysate/extract C. 30 ug NIH-3T3 whole cell lysate/extract D. 30 ug BCL-1 whole cell lysate/extract E. 30 ug Raw264.7 whole cell lysate/extract F. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [261] dilution: 1:1000](http://images.novusbio.com/fullsize/ROCK1-Antibody-261-Western-Blot-NBP2-42917-img0002.jpg)
![Western Blot: ROCK1 Antibody (261) [NBP2-42917] - Analysis of A. 30 ug PC-12 whole cell lysate/extract B. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE ROCK1 antibody [261] dilution: 1:1000.](http://images.novusbio.com/fullsize/ROCK1-Antibody-261-Western-Blot-NBP2-42917-img0003.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: ROCK1 Antibody (261) [NBP2-42917] - Non-transfected (-) and transfected (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 antibody [GT261]](http://images.novusbio.com/fullsize/ROCK1-Antibody-261-Western-Blot-NBP2-42917-img0004.jpg)
![Western Blot: ROCK1 Antibody (261) [NBP2-42917] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK1 (NBP2-42917) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp2-42917_mouse-monoclonal-rock1-antibody-261-910202420122045.jpg)
![Western Blot: ROCK1 Antibody - BSA Free [ROCK1] - Western blot analysis of lysates from wild type(WT) and ROCK1 knockout (KO) 293T cells, using [KO Validated] ROCK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-03713_rabbit-polyclonal-rock1-antibody-5220251636374.jpg "<b>Genetic Strategies Validation. </b>Western Blot: ROCK1 Antibody - BSA Free [ROCK1] - Western blot analysis of lysates from wild type(WT) and ROCK1 knockout (KO) 293T cells, using [KO Validated] ROCK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: ROCK1 Antibody [NBP3-03713] - Paraffin-embedded mouse spleen using ROCK1 antibody at dilution of 1:100 (40x lens).](http://images.novusbio.com/fullsize/ROCK1-Antibody-Immunohistochemistry-Paraffin-NBP3-03713-img0001.jpg)
![Western Blot: ROCK1 Antibody - BSA Free [ROCK1] - Western blot analysis of lysates from C6 cells, using [KO Validated] ROCK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.](http://images.novusbio.com/fullsize/nbp3-03713_rabbit-polyclonal-rock1-antibody-5220251636350.jpg)
![Western Blot: ROCK1 Antibody - BSA Free [ROCK1] - Western blot analysis of various lysates, using [KO Validated] ROCK1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.](http://images.novusbio.com/fullsize/nbp3-03713_rabbit-polyclonal-rock1-antibody-52202516293993.jpg)
![Western Blot: ROCK1 Antibody (2E2) [H00006093-M01] - ROCK1 monoclonal antibody (M01), clone 2E2 Analysis of ROCK1 expression in Hela S3 NE.](http://images.novusbio.com/fullsize/ROCK1-Antibody-2E2-Western-Blot-H00006093-M01-img0008.jpg "Western Blot: ROCK1 Antibody (2E2) [H00006093-M01] - ROCK1 monoclonal antibody (M01), clone 2E2 Analysis of ROCK1 expression in Hela S3 NE.")
![Immunocytochemistry/Immunofluorescence: ROCK1 Antibody (2E2) [H00006093-M01] - Analysis of monoclonal antibody to ROCK1 on HeLa cell. Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/ROCK1-Antibody-2E2-Immunocytochemistry-Immunofluorescence-H00006093-M01-img0006.jpg)
![ELISA: ROCK1 Antibody (2E2) [H00006093-M01] - ROCK1 Antibody (2E2) [H00006093-M01] - Detection limit for recombinant GST tagged ROCK1 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/ROCK1-Antibody-2E2-ELISA-H00006093-M01-img0009.jpg)
![Western Blot: ROCK1 Antibody [NBP1-76569] - 293 cell lysate with ROCK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.](http://images.novusbio.com/fullsize/ROCK1-Antibody-Western-Blot-NBP1-76569-img0001.jpg "Western Blot: ROCK1 Antibody [NBP1-76569] - 293 cell lysate with ROCK1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.")
![Immunocytochemistry/Immunofluorescence: ROCK1 Antibody [NBP1-76569] - Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 ug/mL.](http://images.novusbio.com/fullsize/ROCK1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-76569-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: ROCK1 Antibody - BSA Free [NBP1-76569] - Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 ug/mL.](http://images.novusbio.com/fullsize/nbp1-76569_rabbit-polyclonal-rock1-antibody-271120242010585.jpg)
![Western Blot: ROCK1 Antibody (1H4) [NBP2-37533] - Western blot analysis using ROCK1 mAb against HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/ROCK1-Antibody-1H4-Western-Blot-NBP2-37533-img0003.jpg "Western Blot: ROCK1 Antibody (1H4) [NBP2-37533] - Western blot analysis using ROCK1 mAb against HEK293 (1) and ROCK1 (AA: 403-610)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: ROCK1 Antibody (1H4) [NBP2-37533] - Western blot analysis using ROCK1 mAb against human ROCK1 (AA: 403-610) recombinant protein. (Expected MW is 158 kDa)](http://images.novusbio.com/fullsize/ROCK1-Antibody-1H4-Western-Blot-NBP2-37533-img0002.jpg)
![ELISA: ROCK1 Antibody (1H4) [NBP2-37533] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/ROCK1-Antibody-1H4-ELISA-NBP2-37533-img0001.jpg)
![Western Blot: ROCK1 Antibody (H5E4) [NBP3-15350] - Western blot analysis of extracts of various cell lines, using ROCK1 Rabbit mAb (NBP3-15350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/ROCK1-Antibody-H5E4-Western-Blot-NBP3-15350-img0001.jpg "Western Blot: ROCK1 Antibody (H5E4) [NBP3-15350] - Western blot analysis of extracts of various cell lines, using ROCK1 Rabbit mAb (NBP3-15350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Western Blot: ROCK1 Antibody (H5E4) [ROCK1] - Western blot analysis of various lysates using ROCK1 Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 1s.](http://images.novusbio.com/fullsize/nbp3-15350_rabbit-rock1-mab-h5e4-5220251718917.jpg)