| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12063-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-14160 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20810 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20780 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20794 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Biotin | NBP1-27991 | |||
![Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with CD45RA antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (Ab01502-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/CD45RA-Antibody-OTH-74D4-Chimeric-Flow-Cytometry-NBP3-12063-img0001.jpg "Flow Cytometry: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Using the Anti-CD45RA antibody OTH-74D4 (Ab01502). Paraformaldehyde fixed Jurkat cells permeabilized with 0.5% Triton were stained with CD45RA antibody (Ab00178-23.0; isotype control, black line) or the rabbit IgG version of OTH-74D4 (Ab01502-23.0, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.")
![Immunofluorescence: CD45RA Antibody (OTH-74D4) - Chimeric [NBP3-12063] - Staining of Jukat cells with anti-CD45RA (Ab01502) OTH-74D4 Immunofluorescence analysis of paraformaldehyde fixed Jukat cells on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of OTH-74D4 (Ab01502-23.0) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom Ab01502-23.0, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (Ab00178-23.0) followed by staining with Alexa Fluor® 488 secondary antibody.](http://images.novusbio.com/fullsize/CD45RA-Antibody-OTH-74D4-Chimeric-Immunofluorescence-NBP3-12063-img0002.jpg)
![Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-29202410334038.jpg "Immunocytochemistry/ Immunofluorescence: CD45 Antibody (PSH03-64) [NBP3-32448] - Immunocytochemistry analysis of Raji cells labeling CD45 with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD45 antibody (NBP3-32448) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.")
![Flow Cytometry: CD45 Antibody (PSH03-64) [NBP3-32448] - Flow cytometric analysis of human PBMC labeling human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (NBP3-32448, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32448_mouse-cd45-mab-psh03-64-392024884642.jpg)
![Flow Cytometry: CD45RO Antibody (ILA116A) [NBP2-61035] - Obtained with bovine leukocytes](http://images.novusbio.com/fullsize/CD45RO-Antibody-ILA116A-Flow-Cytometry-NBP2-61035-img0001.jpg "Flow Cytometry: CD45RO Antibody (ILA116A) [NBP2-61035] - Obtained with bovine leukocytes")
![Flow Cytometry: CD45RC Antibody (GL24) [NBP1-27990] - Analysis using the Biotin conjugate of NBP1-27990. Multiple staining of BALB/c mouse splenocytes.](http://images.novusbio.com/fullsize/CD45RC-Antibody-GL24-Flow-Cytometry-NBP1-27990-img0002.jpg "Flow Cytometry: CD45RC Antibody (GL24) [NBP1-27990] - Analysis using the Biotin conjugate of NBP1-27990. Multiple staining of BALB/c mouse splenocytes.")