| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3387-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26877-25ul | |||
| Azide and BSA Free | NBP3-44280 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3387-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65336-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54396-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47784-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20953 | |||
![N/A CD31/PECAM-1 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_CD31_DY80605_ELISA_2523.jpg "N/A CD31/PECAM-1 [Biotin]")
![Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-278202416331211.jpg "Western Blot: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Western blot analysis of CD31/PECAM-1 on different lysates with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. Lane 1: THP-1 cell lysate Lane 2: Jurkat cell lysate Lane 3: U-937 cell lysate Lane 4: HeLa cell lysate (negative) Lane 5: MDA-MB-231 cell lysate (negative) Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 130 kDa Exposure time: 1 minute 58 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32145) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32145) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-298202414115443.jpg)
![Immunocytochemistry/ Immunofluorescence: CD31/PECAM-1 Antibody (7-A1-R) [NBP3-32145] - Immunocytochemistry analysis of THP-1 (positive) and HeLa (negative) labeling CD31/PECAM-1 with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD31/PECAM-1 antibody (NBP3-32145) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594) were used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32145_mouse-cd31-pecam-1-mab-7-a1-r-29202410484878.jpg)
![Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.](http://images.novusbio.com/fullsize/CD31-PECAM1-Antibody-LCI-4-Flow-Cytometry-NB100-65336-img0001.jpg "Flow Cytometry: CD31/PECAM1 Antibody (LCI-4) [NB100-65336] - Staining of porcine peripheral blood lymphocytes.")
![Western Blot: CD31/PECAM-1 Antibody (C31.12) [NBP2-53362] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.12).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-C31-12-Western-Blot-NBP2-53362-img0002.jpg "Western Blot: CD31/PECAM-1 Antibody (C31.12) [NBP2-53362] - Western Blot Analysis of human Jurkat cell lysate using CD31/PECAM-1 Antibody (C31.12).")
![Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (158-2B3) [NBP2-44341] - Immunofluorescent staining of PFA-fixed Jurkat cells using CD31/PECAM-1 Antibody (158-2B3) followed by goat anti-Mouse IgG conjugated with CF488 (green). Nuclei are labeled with Red Dot (red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-158-2B3-Immunocytochemistry-Immunofluorescence-NBP2-44341-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD31/PECAM-1 Antibody (158-2B3) [NBP2-44341] - Immunofluorescent staining of PFA-fixed Jurkat cells using CD31/PECAM-1 Antibody (158-2B3) followed by goat anti-Mouse IgG conjugated with CF488 (green). Nuclei are labeled with Red Dot (red).")
![Flow Cytometry: CD31/PECAM-1 Antibody (158-2B3) [NBP2-44341] - Flow Cytometric Analysis of paraformaldehyde-fixed Jurkat cells using CD31/PECAM-1 Antibody (158-2B3) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/CD31-PECAM-1-Antibody-158-2B3-Flow-Cytometry-NBP2-44341-img0002.jpg)
