NLRP3/NALP3: Novus Biologicals

NBP2-12446

NLRP3/NALP3 Antibody - BSA Free

(4 Reviews)

(250 Publications)

0.2 ml

NBP2-12446

<b>Biological Strategies Validation. </b>Analysis of human esophagus using NLRP3/NALP3 antibody at 1:50 on a Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Cytoplasmic staining in the squamous epithelium was observed. Staining was performed by Histowiz.

Analysis of NLRP3 in cells (Huh-7 and SMMC-7721) exposed luteoloside using anti-NLRP3 antibody. Western blot image submitted by a verified customer review.

Analysis of NALP3 using NALP3 antibody. Human testis lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat testis probed with NALP3 antibody at 5, 2 and 2 ug/mL respectively. Goat anti-rabbit IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Analysis of NALP3 using NALP3 antibody. Lane A) Human NALP3 transfected cell lysate, B) Mouse NALP3 transfected cell lysate, and C) HEK293 control lysate probed with NALP3 antibody at 3 ug/mL.

NLRP3-NALP3-Antibody-Western-Blot-NBP2-12446-img0012.jpg

Effect of Taohong Siwu decoction (THSWD) on the characteristic protein of pyroptosis in middle cerebral artery occlusion-reperfusion (MCAO/R) rats. (A) Photographs of western blots, (B) NLRP3, (C) Caspase-1, (D) Caspase-1 p10, (E) ASC, (F) GSDMD. a: Sham, b: Model, c: THSWD (18 g/kg), d: THSWD (9 g/kg), e: THSWD (4.5 g/kg), f: nimodipine. The results were presented as the mean ± SD (n = 3). Compared with sham group, #p < 0.05, ##p < 0.01. Compared with model group, *p < 0.05, **p < 0.01.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Downstream receptor for advanced glycation end-products (RAGE) & nuclear factor (NF)-kappa B pathway were inhibited after treatment of Nec-1 & melatonin. NF-kappa B pathway was detected by immunoblotting in the cortex (A–E) & hippocampus CA1 (F–J). Relative downstream inflammatory factors were detected by immunoblotting in the cortex (K–N) & hippocampus CA1 (O–R). All experiments were performed in triplicate by one way ANOVA plus Tukey’s test. **P < 0.01 & ***P < 0.001 vs. sham group. #P < 0.05 & ##P < 0.01 vs. CCI group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31607859), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Immunofluorescence staining of TXNIP (a) & NLRP3 (b). NG: normal glucose (5.6 mM); HG: high glucose (30 mM). NH/R: hypoxia (4 h)/reoxygenation (2 h) under NG conditions; HH/R: hypoxia (4 h)/reoxygenation (2 h) under HG conditions. HH/R-RES: HH/R pretreated by RES (50 μM) for 72 h with the high glucose incubation. HH/R-siRNA: TXNIP protein was inhibited by transfection with TXNIP siRNA before HH/R; HH/R-scrambled siRNA: scrambled siRNA used as control before HH/R. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27867451), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - MSC-EVs inhibit activity of NLRP3 inflammasome in SDH of IC rats. A–E Western blot analysis showing that intrathecal injection of MSC-EVs significantly decreased expression levels of NLRP3, Caspase-1, IL-1 beta & IL-18 in SDH of IC rats. n = 8 per group. *P < 0.05 Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35387668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Effect of Taohong Siwu decoction (THSWD) on the characteristic protein of pyroptosis in middle cerebral artery occlusion-reperfusion (MCAO/R) rats. (A) Photographs of western blots, (B) NLRP3, (C) Caspase-1, (D) Caspase-1 p10, (E) ASC, (F) GSDMD. a: Sham, b: Model, c: THSWD (18 g/kg), d: THSWD (9 g/kg), e: THSWD (4.5 g/kg), f: nimodipine. The results were presented as the mean ± SD (n = 3). Compared with sham group, #p < 0.05, ##p < 0.01. Compared with model group, *p < 0.05, **p < 0.01. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33424599), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Hippocampal AMPK/Sirt1 & NF kappa B/NLRP3/IL-1 beta signaling pathways in diabetic rats & the effects of aerobic exercise. Type 2 diabetes significantly decreases the activation of hippocampal AMPK (a) & the level of Sirt1 (c), leading to increased Ac-NF kappa B (d) & NF kappa B (e); diabetic rats contain more NLRP3 inflammasomes (f) & IL-1 beta (g). Aerobic exercise intervention significantly increases AMPK activity & Sirt1 concentration; aerobic exercise downregulates the Ac-NF kappa B & NF kappa B, leading to decreased levels of the NLRP3 inflammasome & IL-1 beta . ∗∗p < 0.01, DM group vs. C group; #p < 0.05, TDM group vs. DM group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30911292), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - The inflammasome inhibitor reduced the myocardial infarct size & decreased activation of the NLRP3 inflammasome, IL-1 beta , & caspase-1 (p10) in nondiabetic & diabetic rats after MI/R. The infarct size was detected by TTC staining (a). The CK-MB activities were determined by enzyme activity assay kits (b). The expression of NLRP3 (c), ASC (d), procaspase-1 & caspase-1 (e), & IL-1 beta (f) was analyzed by Western blot as shown. Data are expressed as the mean ± SD. n = 6 to 8. ∗∗P < 0.01 versus IR. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29062465), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Protein expression in HG & H/R conditions. Western blotting for NLRP3 (a), ASC (b), procaspase-1 & caspase-1 (p10) (c), & IL-1 beta (d) in H9C2 cells. Data are expressed as the mean ± SD. n = 5 per group. ∗∗P < 0.01 versus N; #P < 0.01 versus LG; & ##P < 0.01 versus LG + H/R. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29062465), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - SZF inhibited the activation of the NLRP3-ASC-caspase-1 axis by suppressing TXNIP. Gene & protein expression levels in the renal tissue: (a) TXNIP; (b) NLRP3; (c) ASC; (d) caspase-1 & Procaspase-1. Protein levels were determined by Western blotting, were quantified through densitometry, & are expressed as the optical density ratio to GAPDH. mRNA levels were determined through real-time PCR. The numbers in the Western blot figures represent groups: 1 means Control, 2 means OA, 3 means OA + Allopurinol, & 4 means OA + SZF. Data are expressed as the mean ± SD (n = 3). ∗P < 0.05 versus the OA group. #P > 0.05 versus the OA + Allopurinol group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29358971), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Western blot analysis of TXNIP & NLRP3 protein expression in cultured HK-2 cells treated by normal glucose (5.5 mM), high glucose (30 mM), & NG + mannitol, respectively, for 72 hours, then following 4 hours of hypoxia & 2 hours of reoxygenation in HK-2 cells under high glucose stimulation with or without TXNIP siRNA & RES treatment, respectively. Representative blots (a) & quantitative analysis of Western blots for TXNIP (c) & NLRP3 (d), activity of caspase-1 (e), level of IL-1 beta (f), & Western blot of TXNIP gene knockdown in HK-2 cells (b). The data in (c–f) are means ± SE (n = 5). #P < 0.05 versus NG group; ☆P < 0.05 versus NH/R group; &P < 0.05 versus HH/R-scrambled siRNA group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27867451), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - C-AR inhibited NLRP3 inflammasome activation in synovial tissue. (A,B) Immunohistochemistry staining of NLRP3 expression (A) & cleaved caspase-1 activation (B) in the synovial tissue of collagen-induced arthritis (CIA) rats (original magnification, 100 × ; scale bar, 50 μm). (C,D) IL-1 beta in the synovial tissue of CIA rats (C) & TGF-beta 1-stimulated synovial fibroblasts (D) were measured by ELISA Kits (n = 6); (E) NLRP3 protein expression in succinate-stimulated synovial fibroblasts. The results were derived from four independent experiments for immunohistochemistry staining & Western blot & expressed as the mean ± SD. *p < 0.05 vs. the model; #p < 0.05 vs. the indicated treatment. Image collected & cropped by CiteAb from the following publication (//journal.frontiersin.org/article/10.3389/fimmu.2016.00532/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - The ROS scavenger inhibited the activation of NLRP3 inflammasomes in H9C2 cells exposed to H/R injury. The expression levels of NLRP3 (a), ASC (b), procaspase-1 & caspase-1 (p10) (c), & IL-1 beta (d) were detected by Western blot. Data are expressed as the mean ± SD. n = 5 per group. ∗P < 0.05 & ∗∗P < 0.01 versus LG + H/R; #P < 0.05 & ##P < 0.01 versus HG + H/R. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29062465), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - The activation of the NLRP3 inflammasome & expression of caspase-1 & IL-1 beta were increased in diabetic rats after MI/R insult. NLRP3 & caspase-1 expression in heart tissues were examined by immunohistochemistry (a). The expression of NLRP3 (b), ASC (c), procaspase-1 & caspase-1 (d), & IL-1 beta (e) were analyzed by Western blot. Data are expressed as the mean ± SD. n = 8. ∗∗P < 0.01 versus sham; #P < 0.05 versus Ctrl + sham; & ##P < 0.01 versus Ctrl + I/R. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29062465), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - NLRP3 inflammasome is identified to be significantly upregulated in ipsilateral SCDH of CPIP rats. a Heat map showing the expression of NLR family genes identified in ipsilateral SCDH of CPIP rats vs. sham rats. n = 3 rats/group. b–d qPCR validation of the upregulation of Nlrp3 (b), Caspase-1 (c), & Il-1 beta (d) genes in ipsilateral SCDH of CPIP rats vs. sham rats. n = 8 rats/group. e–g Western blot analysis of NLRP3 (e), Caspase-1 (f), & IL-1 beta (g) protein expressions in ipsilateral SCDH of CPIP rats vs. sham rats. n = 8 rats/group. *p < 0.05, **p < 0.01 vs. sham group. Student’s t test was used for comparisons Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32446302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Exendin-4 inhibits diabetic cardiomyocyte pyroptosis: (a) immunohistochemistry of NLRP3, cleaved caspase-1, IL-1 beta , & IL-18 in the myocardium; (b, c) transcriptional activity of caspase-1 & NLRP3 in the heart (n = 3); (d, e) IL-1 beta & IL-18 ELISA with supernatant of cardiomyocyte culture medium; (f) Western blot of pyroptotic proteins; (g) caspase-1 activity assay (n = 3); (h) FLICA staining in cardiomyocytes. Upper panels showed the fluorescent images of CON, HG, & HG+EXE cardiomyocytes. The lower panel is a statistic of the enrichment of FLICA emitting fluorescence in single cells (n = 10). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31886288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - NLRP3 inflammasome is activated in neurons in SDH of IC rats, & MCC950 inhibits the NLRP3 inflammasome activation. A–E Western blot analysis showing that expression levels of NLRP3, Caspase-1, IL-1 beta & IL-18 were significantly increased in SDH of IC rats compared with normal rats, & MCC950 treatment significantly decreased expression levels of NLRP3, Caspase-1, IL-1 beta & IL-18 in SDH of IC rats. n = 8 per group. *P < 0.05. F Immunofluorescence co-staining showing that NLRP3 was colocalized predominantly with NeuN (neuron marker), but scarcely with GFAP (astrocyte marker) or OX-42 (microglia marker) in the SDH. Scale bars = 200 μm Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35387668), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - The activation of the NLRP3 inflammasome & expression of caspase-1 & IL-1 beta were increased in diabetic rats after MI/R insult. NLRP3 & caspase-1 expression in heart tissues were examined by immunohistochemistry (a). The expression of NLRP3 (b), ASC (c), procaspase-1 & caspase-1 (d), & IL-1 beta (e) were analyzed by Western blot. Data are expressed as the mean ± SD. n = 8. ∗∗P < 0.01 versus sham; #P < 0.05 versus Ctrl + sham; & ##P < 0.01 versus Ctrl + I/R. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29062465), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Exendin-4 inhibits diabetic cardiomyocyte pyroptosis: (a) immunohistochemistry of NLRP3, cleaved caspase-1, IL-1 beta , & IL-18 in the myocardium; (b, c) transcriptional activity of caspase-1 & NLRP3 in the heart (n = 3); (d, e) IL-1 beta & IL-18 ELISA with supernatant of cardiomyocyte culture medium; (f) Western blot of pyroptotic proteins; (g) caspase-1 activity assay (n = 3); (h) FLICA staining in cardiomyocytes. Upper panels showed the fluorescent images of CON, HG, & HG+EXE cardiomyocytes. The lower panel is a statistic of the enrichment of FLICA emitting fluorescence in single cells (n = 10). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31886288), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Glyburide attenuates NLRP3 inflammasome-mediated pyroptosis in HCECs infected with HKCA. HCECs were pretreated with potassium (K+) channel inhibitor (glyburide) for 2 h, & then were incubated with HKCA (MOI = 20) for 24 h. (A,B) Western blot showing the protein levels of NLRP3 in HCECs treated with various concentrations of glyburide (50, 100 & 200 μM) (n = 3). (C,D) Glyburide treatment (200 μM) suppressed the levels of pyroptosis-related proteins (ASC, cleaved CASP1, N-GSDMD, cleaved IL-1 beta & cleaved IL-18) in HCECs challenged with HKCA at 20:1 for 24 h (n = 3). (E) Immunofluorescence analysis of NLRP3, CASP1 & ASC in HCECs pretreated with or without glyburide (200 μM) for 24 h (n = 3). Scale bar = 20 μm; magnification 400×. (F) LDH release of HCECs treated with glyburide (200 μM) (n = 6). CASP1: caspase-1; Clv-CASP1: cleaved CASP1; Clv-IL-1 beta : cleaved IL-1 beta ; Clv-IL-18: cleaved IL-18; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. N.S. P>0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Pharmacological blocking NLRP3 inflammasome activation attenuated the mechanical allodynia of CPIP rats. a Schematic protocol illustrating the time points for model establishment, behavioral tests, & MCC950 (30 μg/rat in 12.5 μl injection volume, via intrathecal catheter)/vehicle (0.1% DMSO in PBS) application. b NLRP3 expression in ipsilateral SCDH of Sham+Veh, CPIP+MCC950, & CPIP+Veh groups measure by Western blot. Upper panel indicates representative images of NLRP3 & beta -actin protein expression. Lower panel indicates summarized NLRP3 expression normalized to beta -actin. c IL-1 beta expression in ipsilateral SCDH of Sham+Veh, CPIP+MCC950, & CPIP+Veh groups measure by Western blot. Upper panel indicates representative images of IL-1 beta & beta -actin protein expression. Lower panel indicates summarized IL-1 beta expression normalized to beta -actin. n = 4 rats/group. d Time course effect of MCC950 on 50% paw withdraw threshold (PWT) of ipsilateral hind paw of CPIP rats. e Summary of the normalized area under the curve (AUC) as in d. n = 6 rats/group. **p < 0.01 vs. Sham+Veh group. ##p < 0.01 vs. CPIP+Veh group. Two-way ANOVA followed by Tukey’s post hoc test was used for comparison in panel d. One-way ANOVA followed by Tukey’s post hoc test was used for comparison in panel e Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32446302), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - The expression of NLRP3 is upregulated in mouse corneas of C. albicans keratitis. C57BL/6 mouse corneas were inoculated with 106 CFU of C. albicans or with sterile PBS & photographed daily after the inoculation. (A) The photographs of mouse C. albicans keratitis were taken by a slit lamp on 0 day (control), 1 day, 3 days, & 7 days post infection (dpi). (B) The clinical score of mouse C. albicans keratitis at different times during C. albicans infection (n = 10). RT-qPCR analysis (C), western blot (D,E) & immunofluorescence staining (F) showing the relative expression of NLRP3 in C. albicans-infected mouse corneas at mRNA (n = 3) & protein levels (n = 3), respectively. Scale bar = 20 μm; magnification 400×. All values are presented as mean ± SEM. *p < 0.05; ***p < 0.001; ****p < 0.0001 vs. control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Lycium barbarum polysaccharides lessened the changes in expression of pyroptosis markers (NOD-like receptors protein 3 (NLRP3), caspase-1, & membrane N-terminal cleavage product of GSDMD (GSDMD-N)) in A beta 1-40 oligomers-exposed ARPE-19 cells. (A) Representative IF images of NLRP3 (green fluorescence) & DAPI (blue fluorescence) in ARPE-19 cells under different treatments. A beta 1-40 oligomers exposure increased the cellular expressions of NLRP3 (second row). However, increased expression was subsequently decreased by LBP treatment with both low (3.5 mg/L) & high (14 mg/L) concentration (third row & the fourth row). (B) The histogram indicated the average fluorescence intensity of NLRP3 based on the IF results (n = 3, ***p < 0.001). (C) Representative IF images of caspase-1 (green fluorescence) & DAPI (blue fluorescence) of ARPE-19 cells in different treatment groups. Expression of caspase-1 in ARPE-19 increased after A beta 1-40 oligomers exposure (second row). Nevertheless, elevated expression was reduced by LBP treatment with both low (3.5 mg/L) & high (14 mg/L) concentration (third & fourth row). (D) The histogram for the average fluorescence intensity of caspase-1 based on the IF data (n = 3, ***p < 0.001). (E) Representative IF images showing expression membrane GSDMD-N (green fluorescence) & DAPI (blue fluorescence) in ARPE-19 cells. There was a remarkable increase in GSDMD-N expression after A beta 1-40 oligomers exposure (second row). Nonetheless, LBP reduced the increased expression at both low (3.5 mg/L) & high (14 mg/L) concentration (third & fourth row). (F) The histogram for the average fluorescence intensity of GSDMD-N based on the IF images (n = 3, ***p < 0.001). Scale bar = 100 µm. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32629957), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - NLRP3 deficiency in non-hematopoietic cells exacerbates GVHD & is not rescued by propionate. B6 WT & Gpr43−/− mice received BMT from either syngeneic B6 or allogeneic BALB/c donors. a Representative immunoblots & densitometric analysis of NLRP3 normalized to the presence of beta -actin in IECs (CD326+) from allogeneic recipients 14 days after BMT (n = 3 each, two-tailed Mann–Whitney U test). b IL-18 production by colon & ileum explant culture from WT B6 & Gpr43−/− mice stimulated overnight with MSU as measured by ELISA (n = 6 each, two-tailed unpaired t-test). c, d Chimeric [B6Ly5.2 → B6] & [B6 Ly5.2 → Nlrp3−/−] animals received BMT from either syngeneic WT B6 or allogeneic BALB/c donors. Survival & clinical GVHD score after BMT (n = 6 syngeneic each, n = 7 allogeneic [B6Ly5.2 → B6], n = 14 [B6 Ly5.2 → Nlrp3−/−], log-rank test for survival, two-tailed Mann–Whitney U test for GVHD Score) are depicted. Data are pooled from two experiments. e, f Chimeric [B6Ly5.2 → B6] & [B6 Ly5.2 → Nlrp3−/−] animals after BMT were treated with vehicle, butyrate (10 mg kg−1 per day) or propionate (15 mg kg−1 per day). Survival & clinical GVHD score after BMT (n = 6 syngeneic, n = 10 Vehicle, n = 5 Butyrate & Propionate each, log-rank test for survival, two-tailed Mann–Whitney U test for GVHD Score). Data are pooled from two experiments. *P < 0.05, **P < 0.01, ***P < 0.001, error bars show the mean ± s.e.m. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30201970), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Glyburide attenuates NLRP3 inflammasome-mediated pyroptosis in HCECs infected with HKCA. HCECs were pretreated with potassium (K+) channel inhibitor (glyburide) for 2 h, & then were incubated with HKCA (MOI = 20) for 24 h. (A,B) Western blot showing the protein levels of NLRP3 in HCECs treated with various concentrations of glyburide (50, 100 & 200 μM) (n = 3). (C,D) Glyburide treatment (200 μM) suppressed the levels of pyroptosis-related proteins (ASC, cleaved CASP1, N-GSDMD, cleaved IL-1 beta & cleaved IL-18) in HCECs challenged with HKCA at 20:1 for 24 h (n = 3). (E) Immunofluorescence analysis of NLRP3, CASP1 & ASC in HCECs pretreated with or without glyburide (200 μM) for 24 h (n = 3). Scale bar = 20 μm; magnification 400×. (F) LDH release of HCECs treated with glyburide (200 μM) (n = 6). CASP1: caspase-1; Clv-CASP1: cleaved CASP1; Clv-IL-1 beta : cleaved IL-1 beta ; Clv-IL-18: cleaved IL-18; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. N.S. P>0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Exercise training prevents HFD-induced upregulation of cardiac pyroptosis. Western blotting was performed to determine the components of inflammasome complex & pyroptosis in the left ventricle of mice hearts. (A) HFD-fed obese mice show upregulation of the NLRP3 inflammasome in the left ventricle, which is prevented by exercise training. (B) Apoptosis-associated speck adaptor protein (ASC) that assembles with NLRP3 & caspase-1 to make inflammasome complex is elevated in the heart of obese mice but attenuated by exercise training. (C) Exercise training prevents HFD-induced upregulation of cardiac caspase-1. (D) Expression of IL-1 beta , which is upregulated by HFD, is normalized by exercise training. All values are expressed as mean ± SEM with dots of different shape representing each animal in a group. One-way ANOVA & Tukey’s post-hoc test were used for statistical analysis. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31835893), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - NLRP3 knockdown decreases corneal inflammation & suppressed neutrophil infiltration in mouse C. albicans keratitis. The C57BL/6 mice were subconjunctivally injected with 6 μL (4 × 108 PFU) of the Ad-GFP-shRNA or Ad-NLRP3-shRNA suspension 3 days before inoculation with 1 × 106 CFU C. albicans or with 5μL sterile PBS after the corneas were scratched. The mouse corneas or eyeballs were collected at 3 dpi & subjected for further detection. RT-qPCR analysis (A), western blot (B) & immunofluorescence staining (C) were used to verify the gene knockdown efficiency of Ad-NLRP3-shRNA (n = 3). Scale bar = 20 μm; magnification 400×. (D) Micrographs of Ad-GFP-shRNA & Ad-NLRP3-shRNA-pretreated mouse corneas were photographed at 3dpi. (E) Clinical score of the infected corneas pretreated with Ad-GFP-shRNA & Ad-NLRP3-shRNA (n = 10). (F) Immunofluorescence staining was performed to assess the levels of neutrophils recruitment in mouse corneas after Ad-GFP-shRNA & Ad-NLRP3-shRNA pretreatment (n =3). Scale bar = 50 μm; magnification 200×. FK: fungal keratitis. All values are presented as mean ± SEM. **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - Heat-killed C. albicans (HKCA) activates NLRP3 inflammasome & induces pyroptosis in human corneal epithelial cells (HCECs). (A) The mRNA expression of NLRP3 in HCECs challenged with HKCA at an MOI of 1:500, 1:50, 1:5, 2:1, or 20:1 respectively for 4 hours was evaluated by RT-qPCR (n = 5). (B–D) The mRNA & protein expression of NLRP3 in HCECs exposed to HKCA (MOI = 20) for 0 (control), 2, 4, 8, 12, or 24 h (n = 3). (E) NLRP3 fluorescence intensity was evaluated using immunofluorescent staining for different times (12–36 h). (n = 3; Scale bar = 20 μm; magnification 400×). (F) Lactate dehydrogenase (LDH) of HCECs treated with HKCA (MOI = 20) for 24 h (n = 6). (G) The mRNA levels of ASC, CASP1, IL-1 beta , IL-18 & GSDMD in HCECs exposed to HKCA (MOI = 20) for different times (n = 3). (H,I) The protein expression of pyroptosis-related proteins (ASC, cleaved CASP1, N-GSDMD, cleaved IL-1 beta & cleaved IL-18) was examined by western blot (n = 3). CASP1: caspase-1; Clv-CASP1: cleaved CASP1; Clv-IL-1 beta : cleaved IL-1 beta ; Clv-IL-18: cleaved IL-18; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - The P2X7R inhibitor suppressed PA-induced inflammation, fibrosis & apoptosis in cardiomyocytes. (A) Cell viability was assessed by the CCK8 assay. H9c2 myocytes were incubated in a medium with A438079 at 0–80 μM for 12 h, & cells without A438079 treatment served as the control. (B) H9c2 myocytes were incubated in a medium with PA at 0–800 μM for 24 h, & cells without PA treatment served as the control. (C) Concentrations of IL-1 beta in cell culture supernatants were detected by ELISA. (D) Representative Western blot analysis of P2X7R, NLRP3 inflammasome, collagen I, MMP9, TGF-beta , caspase-1, caspase-3, Bax, & Bcl-2. (E) The protein semiquantification is shown for P2X7R, NLRP3 & caspase-1. (F) The protein semiquantification is shown for collagen I, MMP9 & TGF-beta . (G) The protein semiquantification is shown for caspase-3, Bax, & Bcl-2. (H) The percentages of TUNEL-positive cells are shown. (I) Representative images of TUNEL stained H9c2 myocytes. CON, H9c2 myocytes without any treatment; PA, H9c2 myocytes treated with 200 μM PA for 24 h; PA + A438079, H9c2 myocytes pretreated with A438079 for 12 h, & then treated with 200 μM PA for 24 h. ∗p < 0.05 versus the CON group, #p < 0.05 versus the HFD group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31681001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP2-12446] - STZ-induced diabetes induces TXNIP expression & NLRP3 inflammasome activation following I/R 48. TXNIP expression was examined by IHC (a), representative blots (b), & quantitative analysis of Western blots for TXNIP (c), NLRP3 (d) & pro-caspase-1 & cleaved caspase-1 (e-f), & release of IL-1 beta & IL-18 by ELISA (g-h). The data in (c–h) are means ± SE (n = 5). ★P < 0.05 versus NS group; #P < 0.05 versus DS group; ▲P < 0.05 versus NI/R group; &P < 0.05 versus DI/R group. NS & DS: nondiabetic & STZ-induced diabetic rats were subjected to sham operation. NI/R & DI/R: nondiabetic & STZ-induced diabetic rats were subjected to 25 min ischemia followed by 48 h reperfusion. DI/R-RES: STZ-induced diabetic rats that underwent I/R were treated with RES (10 mg/kg, ip daily) for 7 consecutive days before renal ischemia-reperfusion. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/27867451), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, RM

Species Glossary

Reactivity: Hu, Mu, Rt, RM

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Alt Catalog#

IMG-6668A

Alt Catalog# IMG-6668A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-12446AF350
Alexa Fluor 405	NBP2-12446AF405
Alexa Fluor 488	NBP2-12446AF488
Alexa Fluor 532	NBP2-12446AF532
Alexa Fluor 594	NBP2-12446AF594
Alexa Fluor 647	NBP2-12446AF647
Alexa Fluor 700	NBP2-12446AF700
Alexa Fluor 750	NBP2-12446AF750
Allophycocyanin	NBP2-12446APC
Biotin	NBP2-12446B
CoraFluor 1	NBP2-12446CL1
DyLight 350	NBP2-12446UV
DyLight 405	NBP2-12446V
DyLight 488	NBP2-12446G
DyLight 550	NBP2-12446R
DyLight 594	NBP2-12446DL594
DyLight 650	NBP2-12446C
DyLight 680	NBP2-12446FR
DyLight 755	NBP2-12446IR
FITC	NBP2-12446F
HRP	NBP2-12446H
Janelia Fluor 525	NBP2-12446JF525
Janelia Fluor 549	NBP2-12446JF549
Janelia Fluor 646	NBP2-12446JF646
Janelia Fluor 669	NBP2-12446JF669
PE	NBP2-12446PE
PerCP	NBP2-12446PCP
mFluor Violet 450 SE	NBP2-12446MFV450
mFluor Violet 500 SE	NBP2-12446MFV500
mFluor Violet 610 SE	NBP2-12446MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-12446SS

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MAB7578

NLRP3/NALP3 Antibody (768319) [Unconjugated]

(67 Publications)

100 μg

MAB7578

HAF005). A specific band was detected for NLRP3/NALP3 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Catalog # AF6789is recommended for use with human lysates." class="big_lightbox">

Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # <a class=

HAF005). A specific band was detected for NLRP3/NALP3 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Catalog # AF6789is recommended for use with human lysates." alt="Western blot shows lysates of J774A.1 mouse reticulum cell sarcoma macrophage cell line and RAW 264.7 mouse monocyte/macrophage cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for NLRP3/NALP3 at approximately 117 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Catalog # AF6789is recommended for use with human lysates." class="big_thumb" />

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox">

NLRP3/NALP3 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # <a class=

NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

NLRP3/NALP3 was detected in immersion fixed paraffin-embedded sections of human tonsil tissue using Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578) at 5 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # <a class=

CTS013). Tissue was stained using the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (brown; Catalog # VC005) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

RAW 264.7 mouse monocyte/macrophage cell line was stained with Rat Anti-Human/Mouse NLRP3/ NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histo-gram) or isotype control antibody (Catalog # <a class=

MAB006, open histo-gram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox">

Human peripheral blood monocytes was stained with Rat Anti-Human/Mouse NLRP3/NALP3 Monoclonal Antibody (Catalog # MAB7578, filled histogram) or isotype control antibody (Catalog # <a class=

MAB006, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

<b>Biological Strategies Validation. </b>Sodium butyrate (NaB) and hydroxybutyric acid (HBA) eyedrops improve clinical parameters and decrease inflammation in animals subjected to alkali burns (AB). (A) Digital images of corneas stained with 0.1% sodium fluorescein demonstrating intact corneal epithelium after topical NaB, HBA or balanced salt solution (BSS) treatment for 5 days quater in die (QID) in untreated mice (n = 5 animals/group). Scale bar: 1000 µm; (B) Representative color digital images used to score corneal opacity (top row) and representative fluorescein stained corneas used to create wound closure rate (bottom row) at 5 days (n = 15 animals/group) after alkali burn. Scale bar: 1000 µm; (C) Corneal opacity in corneas subjected to alkali burn and topically treated with either NaB, HBA, or BSS and compared with dexamethasone (Dex) (n = 15 animals/group); (D) Wound closure rate in corneas subjected to alkali burn and topically treated with either NaB, HBA, or BSS and compared with Dex (n = 15 animals/group); (E) Mean ± SEM of results of gene expression analysis of NLRP3, Caspase-1, and IL-1 beta in whole cornea of animals subjected to alkali burn for 2 or 5 days and topically treated with either NaB, HBA, or BSS and compared with Dex (n = 5 animals/group). * p < 0.05, *** p < 0.001, **** p < 0.0001. (F) Representative digital images of western blot of NLRP3 and beta -actin in cornea epithelium of animals subjected to alkali burn for 2 days and topically treated with either NaB, HBA, or BSS (n = 12 animals/3 samples/group). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28273882), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Upregulation of nucleotide-binding oligomerization domain-containing protein (NOD)-like receptor family pyrin domain containing 3 (NLRP3) expression by corneal epithelium in animals subjected to alkali burn (AB) at 2 days (2 d) and 5 days (5 d) post-injury. (A) Gene expression analysis of NLRP3 messenger mRNA (mRNA) transcript in whole cornea of animals subjected to alkali burn (n = 8 animals/group). Graphs show means ± standard error of the mean (SEM). **** p < 0.0001; (B) Representative merged digital images of laser scanning confocal microscopy of corneas cryosections immunostained for NLRP3 (green) with propidium iodide nuclei counterstaining (DNA in red) in corneas subjected to alkali burn (images are representative of n = 6 animals/group). Scale bar: 50 µm; (C) Representative digital images of western blot of NLRP3 and beta -actin in cornea epithelium of animals subjected to alkali burn for 2 and 5 days. Each lane is a different sample (n = 12 animals/3 samples/group). MW: Molecular weight; UT: untreated control; 2 d and 5 d refer to mice subjected to alkali burn and euthanized 2 or 5 days post-injury, respectively (1 and 2 indicate different samples). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/28273882), licensed under a CC-BY license. Not internally tested by R&D Systems.

NLRP3 inflammasome-dependent pyroptosis occurs in liver fibrosis. (A) IHC staining for GSDMD, IL-1 beta , and IL-18 in liver sections from liver fibrosis patients and HCs. Scale bar: 40 µm. (B–D) ELISA analyses of serum levels of GSDMD (B), IL-1 beta (C), and IL-18 (D) in liver fibrosis patients (n = 89) and HCs (n = 60). (E) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the human fibrotic liver tissues. Scale bar: 40 µm. (F) Schematic diagram of the study. Liver fibrosis was induced by CCl4 injection for 8 weeks. (G) Representative mouse liver histology of H&E, Sirius Red staining, and IHC staining for alpha -SMA, GSDMD, and IL-1 beta . Black scale bar: 100 µm; Red scale bar: 50 µm. (H–J) ELISA analyses for serum levels of GSDMD (H), IL-1 beta (I), and IL-18 (J) in CCl4 group mouse (n = 5) and vehicle group mouse (n = 5). (K) Representative immunofluorescence images of NLRP3 (red) and albumin (hepatocyte marker) (top), F4/80 (KC marker) (middle) or alpha -SMA (HSC marker) (bottom) (green) from the 8-week CCl4-treated mouse liver. The vehicle group mouse liver was used as a control. Scale bar: 40 µm. (L) The qRT-PCR analysis for mRNA levels of IL-1 beta in THP-1 macrophages treated with LPS to induce pyroptosis. (M) ELISA analysis for IL-1 beta expression in supernatants from THP-1. (N) Western blot analysis of COL1A1, alpha -SMA, and TGF-beta expression in LX-2 cells which were exposed to CM from LPS-treated THP-1 macrophages. The protein expression was quantified by densitometry and normalized to beta -actin and are shown as fold changes relative to the control group (right panel). ** p < 0.01, *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36429008), licensed under a CC-BY license. Not internally tested by R&D Systems.

DAMP S100A8 along with NLRP3 inflammasome-dependent pyroptosis is positively related to the progression of liver fibrosis. (A) Representative IHC images for S100A8 and S100A9 in liver sections from liver fibrosis patients and HCs. (B,C) ELISA analyses for serum levels of S100A8 and S100A9 in liver fibrosis patients and HCs. (D) Comparison of serum S100A8 and S100A9 levels in liver fibrosis patients with different phases. (E–G) Distribution of serum GSDMD (E), IL-1 beta (F), and IL-18 (G) levels in liver fibrosis patients with different phases (F0–4). (H–J) Correlation between serum S100A8 levels and GSDMD (H), IL-1 beta (I) or IL-18 (J) levels in liver fibrosis patients. (K) Representative mouse liver morphology and staining with H&E and Sirius Red. (L–O) IHC staining of mouse liver sections for NLRP3, S100A8, and S100A9. Black scale bar: 100 µm; Red scale bar: 50 µm. ELISA analyses for serum levels of S100A8 (L), GSDMD (M), IL-1 beta (n), and IL-18 (O) in 4-, 6-, and 8 week-mouse models of liver fibrosis. *** p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36429008), licensed under a CC-BY license. Not internally tested by R&D Systems.

Inhibition of NLRP3 inflammasome-dependent pyroptosis alleviates liver fibrosis progression. (A) Experimental protocol of NLRP3 inhibitor MCC950 or saline application based on CCl4 injection in mice. (B) Representative liver histology of H&E and Sirius Red staining. The expression of alpha -SMA, NLRP3, GSDMD, and IL-1 beta was determined by immunohistochemistry. Black scale bar: 100 µm; Red scale bar: 50 µm. (C–E) Serum levels of ALT, AST, and TP were measured. * p < 0.05. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36429008), licensed under a CC-BY license. Not internally tested by R&D Systems.

Expression of NLRP3, caspase‐1 and gasdermin D‐N‐terminal (GSDMD‐N) in synovial tissues from patients with osteoarthritis (OA) or rheumatoid arthritis (RA). (A) Sections were stained with antibodies against the indicated proteins. Scale bar, 100 μm. (B) Sections were stained with an antibody against the macrophage marker CD68 and another antibody against a pyroptosis‐related marker. Nuclei were counterstained with DAPI. Scale bar, 50 μm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37386795), licensed under a CC-BY license. Not internally tested by R&D Systems.

Effect of ginsenoside Rh4 on NLRP3 expression in LPS and nigericin-induced microglia cells. (A): NLRP3 (red) and nuclei (blue) using a laser confocal microscope; (B): Quantitative analysis of NLRP3 fluorescence intensity. Scale bars, 70 μm. The bar represents the mean ± standard error of the mean (n = 3). The Student’s t-test determined significant differences (### p < 0.001 compared with normal cells, *** p < 0.001 with LPS and nigericin-treated cells). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/37239965), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , ,

Applications: , , , ,

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC7578U-100UG
Alexa Fluor 488	IC7578G
Alexa Fluor 532	FAB7578X
Alexa Fluor 594	IC7578T-100UG
Alexa Fluor 647	IC7578R-100UG
Alexa Fluor 700	IC7578N
Alexa Fluor 750	IC7578S-100UG
Alexa Fluor Plus 405	FAB7578AFP405-100UG
Alexa Fluor Plus 488	FAB7578AFP488-100UG
Alexa Fluor Plus 555	FAB7578AFP555-100UG
Alexa Fluor Plus 594	FAB7578AFP594-100UG
Alexa Fluor Plus 647	FAB7578AFP647-100UG
Alexa Fluor Plus 680	FAB7578AFP680-100UG
Allophycocyanin	IC7578A
Biotin	FAB7578B
CoraFluor 1	FAB7578CL1
DyLight 350	FAB7578D
DyLight 405	FAB7578E
DyLight 488	FAB7578K
DyLight 550	FAB7578L
DyLight 594	FAB7578M
DyLight 650	FAB7578W
DyLight 680	FAB7578Y
DyLight 755	FAB7578Z
FITC	FAB7578F
HRP	FAB7578H
Janelia Fluor 525	FAB7578JF525
Janelia Fluor 549	FAB7578I
Janelia Fluor 646	FAB7578J
Janelia Fluor 669	FAB7578JF669
Phycoerythrin	IC7578P
mFluor Violet 450 SE	FAB7578MFV450
mFluor Violet 500 SE	FAB7578MFV500
mFluor Violet 610 SE	FAB7578MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC7578P
Carrier Free	IC7578A
Carrier Free	IC7578G
Carrier Free	IC7578N
Carrier Free	MAB7578-SP
Carrier Free	IC7578U-100UG
Carrier Free	IC7578T-100UG
Carrier Free	IC7578S-100UG
Carrier Free	IC7578R-100UG

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NBP1-77080

NLRP3/NALP3 Antibody - BSA Free

(2 Reviews)

(35 Publications)

0.1 mg

NBP1-77080

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in mouse lung tissue using anti-NALP3 antibody. Image from verified customer review.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 ug/mL (A) in the absence and (B) in the presence of blocking peptide.

<b>Biological Strategies Validation. </b>NLRP3-NALP3 Antibody - BSA Free-Immunocytochemistry-Immunofluorescence-NBP1-77080-img0012.jpg

<b>Biological Strategies Validation. </b>hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal and conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva and cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05.

Immunocytochemistry/ Immunofluorescence: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - hADSC-Evs suppressed NLRP3 inflammasome activation in ocular surface of experimental mice under desiccating stress. (A) Representative image of immunofluorescent staining of NLRP3 related genes in corneal & conjunctival epithelia of desiccating mice or treated with hADSC-EVs (*conjunctiva; open triangle cornea). (B,C) qRT-PCR showed the mRNA levels of NLRP3 related genes in conjunctiva & cornea (four corneas/conjunctivas were mixed as a sample). Data was shown as mean ± SEM. *p < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/32884023), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 promoted the activation of canonical NLRP3 inflammasome in retinal IR injury. The intravitreal injection of exogenous rHMGB1 significantly promoted the mRNA & protein production of NLRP3 (a, c) & ASC (e, g) & activation of caspase-1 (i). Thus, the intravitreal injection of HMGB1 inhibitor, GA, significantly suppressed the production of NLRP3 (b, d) & ASC (f, h) & activation of caspase-1 (j). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - Co-immunoprecipitation assay: Figure showing interaction of NLRP3, NLRC4 & NAIP with ASC during CFT073 infection in THP-1m. Cytoplasmic extracts of THP-1m infected with or without CFT073, 6 h post stimulation were subjected to IP & WB by various antibodies (ASC, NLRP3, NLRC4, & NAIP) as described in material & methods. Cytoplasmic extract used as input was subjected to check NLRP3, ASC, NLRC4 & NAIP expression as shown in figure. ASC antibody was used to co-immunoprecipitate the complex from cytoplasmic extracts & then immunoblotted to detect NLRP3, NLRC4 & NAIP. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31551961), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody - BSA Free [NBP1-77080] - HMGB1 regulated the activation of NLRP3, rather than the activation of caspase-8, via NF-kappa B pathway. a, b NF-kappa B was activated in ischemic retina at the early stage after reperfusion. c, d Intraocular injection rHMGB1 or GA could promote or suppress the production of phosphor-NF-kappa B p65. e, f Intravitreal injection of NF-kappa B p65 inhibitor, JSH-23, significantly reduced the activation of NLRP3 & decreased the processing of IL-1 beta , g rather than caspase-8. However, intravitreal injection of caspase-8 inhibitor, Z-IETD-fmk, decreased the production of phosphor-NF-kappa B p65, obviously (h). Data are shown as mean ± SD. *P < 0.05, **P < 0.01 vs sham group Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26224068), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Ec

Species Glossary

Reactivity: Hu, Mu, Ec

Applications:

, , , , ,

Applications: , , , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP1-77080AF350
Alexa Fluor 405	NBP1-77080AF405
Alexa Fluor 488	NBP1-77080AF488
Alexa Fluor 532	NBP1-77080AF532
Alexa Fluor 594	NBP1-77080AF594
Alexa Fluor 647	NBP1-77080AF647
Alexa Fluor 700	NBP1-77080AF700
Alexa Fluor 750	NBP1-77080AF750
Alexa Fluor Plus 405	NBP1-77080AFP405
Alexa Fluor Plus 488	NBP1-77080AFP488
Alexa Fluor Plus 555	NBP1-77080AFP555
Alexa Fluor Plus 594	NBP1-77080AFP594
Alexa Fluor Plus 647	NBP1-77080AFP647
Alexa Fluor Plus 680	NBP1-77080AFP680
Allophycocyanin	NBP1-77080APC
Biotin	NBP1-77080B
CoraFluor 1	NBP1-77080CL1
DyLight 350	NBP1-77080UV
DyLight 405	NBP1-77080V
DyLight 488	NBP1-77080G
DyLight 550	NBP1-77080R
DyLight 594	NBP1-77080DL594
DyLight 650	NBP1-77080C
DyLight 680	NBP1-77080FR
DyLight 755	NBP1-77080IR
FITC	NBP1-77080F
HRP	NBP1-77080H
Janelia Fluor 525	NBP1-77080JF525
Janelia Fluor 549	NBP1-77080JF549
Janelia Fluor 646	NBP1-77080JF646
Janelia Fluor 669	NBP1-77080JF669
PE	NBP1-77080PE
PerCP	NBP1-77080PCP
mFluor Violet 450 SE	NBP1-77080MFV450
mFluor Violet 500 SE	NBP1-77080MFV500
mFluor Violet 610 SE	NBP1-77080MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-77080SS

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NBP2-67639

NLRP3/NALP3 Antibody (SC06-23)

(4 Publications)

100 ul

NBP2-67639

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human liver carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of Jurkat cells with NLRP3 antibody at 1:50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HeLa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunocytochemistry/Immunofluorescence: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Staining NLRP3 in PMVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X-100 in PBS.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse bladder tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded mouse spleen tissue using anti- NLRP3 antibody. Counter stained with hematoxylin.

<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human lung carcinoma tissue using anti-NLRP3/NALP3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody (SC06-23) [NBP2-67639] - Analysis of paraffin-embedded human colon carcinoma tissue with Rabbit anti-NLRP3/NALP3 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , , ,

Applications: , , , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-97601

NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free

(7 Publications)

0.05 mg

NBP1-97601

Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression in VSMCs causes inflammation & lipid deposition in the aortas of SCAPD443N mice. (A & B) Representative images of an aortic root stained with Oil Red O in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding. (C) Quantification of plaque areas in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (D) Plasma levels of TC, TG, LDL-C & HDL-C in SCAPD443N mice & SCAP+/+ mice after 12 or 24 weeks of Western diet feeding (n=5). (E) mRNA expression of proin?ammatory cytokines (IL-1 beta , IL-6, IL-18, & TNF-alpha ) as measured by qRT-PCR (n=4). (F) mRNA expression of pro-chemokine cytokines (MCP-1, MIP-1 alpha , MIP-1 beta , & CX3CL1) as measured by qRT-PCR (n=4). (G) mRNA expression of ICAM-1 & VCAM-1 in aortas as measured by qRT-PCR (n=4). (H) Representative immunoblots for NLRP3 proteins in SCAP+/+ mice & SCAPD443N mice (n=3). (I) Representative images of immuno?uorescence staining of NLRP3 proteins in the aortas of SCAPD443N mice & SCAP+/+ mice (n=5). The data are presented as the means±SD of 3-5 independent experiments. *P<0.05, vs. the SCAP+/+ group. Statistical significance was calculated for the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free [NBP1-97601] - Sterol-resistant SCAP overexpression induces the activation of NLRP3 in?ammasomes in VSMCs. (A) Representative immunoblots for NLRP3, Procaspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs. (B) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (C) Immunoblotting of immunoprecipitation with anti-SCAP or anti-NLRP3 in SCAP-overexpressing VSMCs. (D) Representative images of immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi(red) in control & SCAP-overexpressing VSMCs. (E) Representative immunoblot for NLRP3, Pro-caspase-1, cleaved caspase-1, IL-1 beta , & IL-18 in SCAP-overexpressing VSMCs treated with lycorine for 4 h. (F) Expression of IL-1 beta & IL-18 in VSMC supernatants as measured by ELISA. (G) Representative images of the immuno?uorescence staining of NLRP3 (green) or SCAP (green) with Golgi (red) in SCAP VSMCs after treatment with lycorine for 4 h. The data are presented as the means±SD of 3 independent experiments. *P<0.05, vs the control group. Statistical significance was calculated for to the biological replicates by 2-tailed Student’s t test. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/34094640), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-97601SS

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NBP2-03948

NLRP3/NALP3 Antibody (25N10E9) - BSA Free

(3 Publications)

0.1 mg

NBP2-03948

Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis using the NALP3 monoclonal antibody in mouse NALP3 transfected cell lysate (A), human NALP3 transfected cell lysate (B), mouse RAW lysate (C) and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Alt Catalog#

IMG-6763A

Alt Catalog# IMG-6763A

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-03948AF350
Alexa Fluor 405	NBP2-03948AF405
Alexa Fluor 488	NBP2-03948AF488
Alexa Fluor 532	NBP2-03948AF532
Alexa Fluor 594	NBP2-03948AF594
Alexa Fluor 647	NBP2-03948AF647
Alexa Fluor 700	NBP2-03948AF700
Alexa Fluor 750	NBP2-03948AF750
Alexa Fluor Plus 405	NBP2-03948AFP405
Alexa Fluor Plus 488	NBP2-03948AFP488
Alexa Fluor Plus 555	NBP2-03948AFP555
Alexa Fluor Plus 594	NBP2-03948AFP594
Alexa Fluor Plus 647	NBP2-03948AFP647
Alexa Fluor Plus 680	NBP2-03948AFP680
Allophycocyanin	NBP2-03948APC
Biotin	NBP2-03948B
CoraFluor 1	NBP2-03948CL1
DyLight 350	NBP2-03948UV
DyLight 405	NBP2-03948V
DyLight 488	NBP2-03948G
DyLight 550	NBP2-03948R
DyLight 594	NBP2-03948DL594
DyLight 650	NBP2-03948C
DyLight 680	NBP2-03948FR
DyLight 755	NBP2-03948IR
FITC	NBP2-03948F
HRP	NBP2-03948H
Janelia Fluor 525	NBP2-03948JF525
Janelia Fluor 549	NBP2-03948JF549
Janelia Fluor 646	NBP2-03948JF646
Janelia Fluor 669	NBP2-03948JF669
PE	NBP2-03948PE
PerCP	NBP2-03948PCP
mFluor Violet 450 SE	NBP2-03948MFV450
mFluor Violet 500 SE	NBP2-03948MFV500
mFluor Violet 610 SE	NBP2-03948MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-03948SS
Azide and BSA Free	NBP2-80881

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NBP2-52879

NLRP3/NALP3 Antibody (CL0210) - BSA Free

(1 Publication)

0.1 ml

NBP2-52879

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-44085AF350
Alexa Fluor 405	NBP3-44085AF405
Alexa Fluor 488	NBP3-44085AF488
Alexa Fluor 532	NBP3-44085AF532
Alexa Fluor 594	NBP3-44085AF594
Alexa Fluor 647	NBP3-44085AF647
Alexa Fluor 700	NBP3-44085AF700
Alexa Fluor 750	NBP3-44085AF750
Alexa Fluor Plus 405	NBP3-44085AFP405
Alexa Fluor Plus 488	NBP3-44085AFP488
Alexa Fluor Plus 555	NBP3-44085AFP555
Alexa Fluor Plus 594	NBP3-44085AFP594
Alexa Fluor Plus 647	NBP3-44085AFP647
Alexa Fluor Plus 680	NBP3-44085AFP680
Allophycocyanin	NBP3-44085APC
Biotin	NBP3-44085B
CoraFluor 1	NBP3-44085CL1
DyLight 350	NBP3-44085UV
DyLight 405	NBP3-44085V
DyLight 488	NBP3-44085G
DyLight 550	NBP3-44085R
DyLight 594	NBP3-44085DL594
DyLight 650	NBP3-44085C
DyLight 680	NBP3-44085FR
DyLight 755	NBP3-44085IR
FITC	NBP3-44085F
HRP	NBP3-44085H
Janelia Fluor 525	NBP3-44085JF525
Janelia Fluor 549	NBP3-44085JF549
Janelia Fluor 646	NBP3-44085JF646
Janelia Fluor 669	NBP3-44085JF669
PE	NBP3-44085PE
PerCP	NBP3-44085PCP
mFluor Violet 450 SE	NBP3-44085MFV450
mFluor Violet 500 SE	NBP3-44085MFV500
mFluor Violet 610 SE	NBP3-44085MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-44085
BSA Free	NBP2-52879-25ul

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AF6789

NLRP3/NALP3 Antibody [Unconjugated]

(2 Publications)

100 μg

AF6789

HAF016). A specific band was detected for NLRP3/NALP3 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with human NALP1 or transfected with NLRP3/NALP3 and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 20 ng/mL PMA for 72 hours and 1 µg/mL LPS for 24 hours. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6789) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). A specific band was detected for NLRP3/NALP3 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected, transfected with human NALP1 or transfected with NLRP3/NALP3 and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 20 ng/mL PMA for 72 hours and 1 µg/mL LPS for 24 hours. PVDF Membrane was probed with 0.5 µg/mL of Sheep Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6789) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NLRP3/NALP3 at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2." class="big_thumb" />

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

<b>Biological Strategies Validation. </b>NLRP3/NALP3 was detected in immersion fixed THP-1 human acute monocytic leukemia cells, unstimulated (lower panel) and stimulated (upper panel) with PMA and LPS, using Sheep Anti-Human NLRP3/NALP3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6789) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # <a class=

NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

<b>Biological Strategies Validation. </b>Simple Western lane view shows lysates of THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with PMA and LPS, loaded at 0.2 mg/mL. A specific band was detected for NLRP3/NALP3 at approximately 111 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human NLRP3/NALP3 (aa 4-223) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6789) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # <a class=

HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

NLRP3s expression in Monocyte by Western blot: NLRP3s protein expression assessed by western blotting in monocyte of individuals with a diagnosis of either severe Alzheimer’s disease (AD), moderate Alzheimer’s disease (MILD) or Mild Cognitive Impairment (MCI) and of age- and sex-matched Healthy Controls (HC). The same protein concentration of whole-cells lysates was loaded into the gel, as confirmed by actin. Representative results obtained in un-stimulated or in LPS-primed and A beta 42-stimulated monocytes are presented in the upper panel. Quantitative evaluation (arbitrary unit, AU) of NLRP3s expression obtained comparing band density (normalized to actin) in un-stimulated or in LPS-primed A beta 42-stimulated monocytes is shown in the lower panel. (N = 5, mean ± s.e.m., T test, *p <0.05). (N = 5/group, mean ± S.E.M and statistical significance are shown. *(p <0.05) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26939933), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF6789U-100UG
Alexa Fluor 405	AF6789V-100UG
Alexa Fluor 488	AF6789G-100UG
Alexa Fluor 532	AF6789X-100UG
Alexa Fluor 594	AF6789T-100UG
Alexa Fluor 647	AF6789R-100UG
Alexa Fluor 700	AF6789N-100UG
Alexa Fluor 750	AF6789S-100UG
Alexa Fluor Plus 405	AF6789AFP405-100UG
Alexa Fluor Plus 488	AF6789AFP488-100UG
Alexa Fluor Plus 555	AF6789AFP555-100UG
Alexa Fluor Plus 594	AF6789AFP594-100UG
Alexa Fluor Plus 647	AF6789AFP647-100UG
Alexa Fluor Plus 680	AF6789AFP680-100UG
Allophycocyanin	AF6789A
Biotin	AF6789B
CoraFluor 1	AF6789CL1
DyLight 350	AF6789D
DyLight 405	AF6789E
DyLight 488	AF6789K
DyLight 550	AF6789L
DyLight 594	AF6789M
DyLight 650	AF6789W
DyLight 680	AF6789Y
DyLight 755	AF6789Z
FITC	AF6789F
HRP	AF6789H
Janelia Fluor 525	AF6789JF525
Janelia Fluor 549	AF6789I
Janelia Fluor 646	AF6789J
Janelia Fluor 669	AF6789JF669
PE	AF6789P
PerCP	AF6789C
mFluor Violet 450 SE	AF6789MFV450
mFluor Violet 500 SE	AF6789MFV500
mFluor Violet 610 SE	AF6789MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF6789-SP

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H00114548-P01

Recombinant Human NLRP3/NALP3 GST (N-Term) Protein

(10 Publications)

2 μg

H00114548-P01

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, , , ,

Applications: , , , ,

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NBP1-90207

NLRP3/NALP3 Antibody - BSA Free

(6 Publications)

0.1 ml

NBP1-90207

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP1-90207] - Staining of human spleen shows cytoplasmic positivity in lymphoid cells.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP1-90207-25ul

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NBP3-18216

Human NLRP3/NALP3 ELISA Kit (Colorimetric)

1 Kit

NBP3-18216

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Standard Curve:

0.31-20 ng/ml

Sensitivity:

0.16 ng/ml

Sample Type:

Serum, plasma, cell culture supernatants, cell lysates, tissue homogenates and other biological fluids

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NB100-41104

NLRP3/NALP3 Antibody

(3 Publications)

0.1 mg

NB100-41104

Activation of NLRP3 inflammasome in platelets from patients with active Crohn’s disease (CD). (A) Co-immunoprecipitation of ASC with NLRP3 in platelets from the healthy group and patients with active CD (i). Quantification of the interaction between ASC and NLRP3 in platelets was below (n = 3), (ii). (B) Immunofluorescence staining showed colocalization of ASC (green) and NLRP3 (red) in platelets from patients with active CD (i). The experiment was repeated three times. Intensity traces were plotted below (ii). Scale bars were 20 μm. Health, the healthy group; CD, the patients with active Crohn’s disease. Data are presented as mean +/- SD, ***p < 0.001. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/34262463), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Mu, Rt, Po, Bv, Ca

Species Glossary

Reactivity: Hu, Mu, Rt, Po, Bv, Ca

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

NLRP3 Products By Gene Name

NLRP3/NALP3 Antibody - BSA Free

NLRP3/NALP3 Antibody (768319) [Unconjugated]

NLRP3/NALP3 Antibody - BSA Free

NLRP3/NALP3 Antibody (SC06-23)

NLRP3/NALP3 Antibody (Nalpy3-b) - BSA Free

NLRP3/NALP3 Antibody (25N10E9) - BSA Free

NLRP3/NALP3 Antibody (CL0210) - BSA Free

NLRP3/NALP3 Antibody [Unconjugated]

Recombinant Human NLRP3/NALP3 GST (N-Term) Protein

NLRP3/NALP3 Antibody - BSA Free

Human NLRP3/NALP3 ELISA Kit (Colorimetric)

NLRP3/NALP3 Antibody