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![ELISA p38 alpha [Biotin]](http://images.novusbio.com/fullsize2/dyc8691b-2_total-p38-alpha-duoset-ic-elisa-2-plate-elisa-169202013418.jpg "ELISA p38 alpha [Biotin]")
![Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-288202412442950.jpg "Western Blot: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Western blot analysis of p38 alpha on different lysates with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa treated with UV for 1 hour cell lysate Lane 3: HeLa treated with UV for 1 hour cell lysate, then the membrane treated with pp for 1 hour Lane 4: PC-12 cell lysate Lane 5: PC-12 treated with UV for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 41 kDa Observed band size: 38 kDa Exposure time: 2 minutes; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32781) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunohistochemical analysis of paraffin-embedded human breast cancer tissue untreated / treated with lambda pp / phospho-peptide / non-phospho-peptide with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32781) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-298202414333624.jpg)
![Immunocytochemistry/ Immunofluorescence: p38 alpha [p Thr180] Antibody (JE77-37) [NBP3-32781] - Immunocytochemistry analysis of Jurkat cells treated with or without UV for 1 hour labeling p38 alpha with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-p38 alpha antibody (NBP3-32781) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize/nbp3-32781_rabbit-p38-alpha-p-thr180-mab-je77-37-2920241114829.jpg)
![Western Blot: p38 alpha [p Tyr323] Antibody [NBP3-23249] - A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38 alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38 alpha (Tyr-323) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23249_rabbit-p38-alpha-p-tyr323-pab-19920231410135.jpg "Western Blot: p38 alpha [p Tyr323] Antibody [NBP3-23249] - A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38 alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38 alpha (Tyr-323) (lanes 3 & 4).")
![Immunocytochemistry/Immunofluorescence: p38 alpha [p Tyr323] Antibody [NBP3-23249] - Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23249_rabbit-p38-alpha-p-tyr323-pab-19920231911472.jpg)
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M138) [NBP3-23233] - Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.](http://images.novusbio.com/fullsize/nbp3-23233_mouse-p38-alpha-mab-m138-19920231410114.jpg "Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M138) [NBP3-23233] - Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and p38 MAPK (T180/Y182) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.")
![Western Blot: p38 alpha Antibody (M138) [NBP3-23233] - Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38 alpha (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phospho-p38 (T180/Y182) peptide (lane 7).](http://images.novusbio.com/fullsize/nbp3-23233_mouse-p38-alpha-mab-m138-19920231252360.jpg)
![Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-199202318202923.jpg "Immunocytochemistry/Immunofluorescence: p38 alpha Antibody (M548) [NBP3-23234] - Immunocytochemical labeling of p38 alpha in aldehyde fixed and NP-40 permeabilized human NCI-H446 lung carcinoma cells. The cells were labeled with mouse monoclonal anti-p38 alpha (NBP3-23234). The antibody was detected using goat anti-mouse DyLight(R) 594.")
![Western Blot: p38 alpha Antibody (M548) [NBP3-23234] - Western blot analysis of human A431 cells (lanes 1 & 2) and rat A7r5 cells (lanes 3 & 4). The blot was probed with mouse monoclonal anti-p38 alpha (C-terminal) clone M548 at a dilution of 1:250 (lanes 1 & 3) or 1:1000 (lanes 2 & 4).](http://images.novusbio.com/fullsize/nbp3-23234_mouse-p38-alpha-mab-m548-19920231856916.jpg)
![Western Blot: p38 alpha [p Tyr182, p Thr180] Antibody (RM243) [NBP2-61574] - Western Blot of HeLa cell lysates, treated or nontreated with anisomycin, using NBP2-61574, showed a band of Phospho-p38 MAPK (~38 kDa) in anisomycin-treated HeLa cells.](http://images.novusbio.com/fullsize/p38-alpha-p-Tyr182-p-Thr180-Antibody-RM243-Western-Blot-NBP2-61574-img0003.jpg "Western Blot: p38 alpha [p Tyr182, p Thr180] Antibody (RM243) [NBP2-61574] - Western Blot of HeLa cell lysates, treated or nontreated with anisomycin, using NBP2-61574, showed a band of Phospho-p38 MAPK (~38 kDa) in anisomycin-treated HeLa cells.")
![Immunohistochemistry-Paraffin: p38 alpha [p Tyr182, p Thr180] Antibody (RM243) [NBP2-61574] - Immunohistochemical staining of formalin fixed and paraffin embedded human breast cancer tissue sections using NBP2-61574.](http://images.novusbio.com/fullsize/p38-alpha-p-Tyr182-p-Thr180-Antibody-RM243-Immunohistochemistry-Paraffin-NBP2-61574-img0002.jpg)
![Immunohistochemistry-Paraffin: p38 alpha Antibody (0004) [NBP3-23507] - Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using NBP3-23507 at 1:500 dilution.](http://images.novusbio.com/fullsize/nbp3-23507_rabbit-p38-alpha-mab-0004-1362024149311.png "Immunohistochemistry-Paraffin: p38 alpha Antibody (0004) [NBP3-23507] - Immunohistochemical analysis of paraffin-embedded human carcinoma of sigmoid tissue,untreated (left) or lambda phosphatase-treated (right), using NBP3-23507 at 1:500 dilution.")
![Immunocytochemistry/ Immunofluorescence: p38 alpha Antibody (0004) [NBP3-23507] - Immunofluorescence staining of p38 alpha in serum-starved NIH-3T3 cells, untreated (left), treated with UV (30mins) (middle) or treated with UV (30mins) and lambda phosphatase (right). Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP3-23507 (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Goat Anti-rabbit IgG secondary antibody [Alexa Fluor 488] (green). Positive staining was mainly localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-23507_rabbit-p38-alpha-mab-0004-12620241540460.png)
![Western Blot: p38 alpha Antibody (0004) [NBP3-23507] - Western blot analysis of extracts from serum-starved NIH-3T3, untreated (line A); treated with UV (30 min), without peptide (line B) or antigen-specific phosphopeptide (line C) or antigen-specific peptide (line D) using NBP3-23507 at 1:1000 dilution.](http://images.novusbio.com/fullsize/nbp3-23507_rabbit-p38-alpha-mab-0004-13620242234504.png)
![Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).](http://images.novusbio.com/fullsize/nbp3-23232_rabbit-p38-alpha-pab-199202312403713.jpg "Western Blot: p38 alpha Antibody [NBP3-23232] - Western blot analysis of p38 MAP kinase in mouse macrophage (J774A.1) cell lysate (lanes 1-4). The blots were probed with mouse monoclonal anti-p38 alpha (C-terminal) at 1:500 (lane 1) and 1:2000 (lane 2) or rabbit polyclonal anti-p38 alpha (a.a. 319-328) at 1:250 (lane 3) and 1:1000 (lane 4).")
![Western Blot: pan-p38 Antibody (PSH03-72) [NBP3-33148] - Western blot analysis of pan-p38 on different lysates with Rabbit anti-pan-p38 antibody (NBP3-33148) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: COS-1 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33148) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33148_rabbit-pan-p38-mab-psh03-72-28820241435150.jpg "Western Blot: pan-p38 Antibody (PSH03-72) [NBP3-33148] - Western blot analysis of pan-p38 on different lysates with Rabbit anti-pan-p38 antibody (NBP3-33148) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: COS-1 cell lysate Lane 5: Neuro-2a cell lysate Lane 6: PC-12 cell lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 4 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33148) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: p38 alpha/beta/gamma Antibody (PSH03-60) [NBP3-33147] - Western blot analysis of p38 alpha/beta/gamma on different lysates with Rabbit anti-p38 alpha/beta/gamma antibody (NBP3-33147) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: COS-1 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse skeletal muscle tissue lysate Lane 9: Rat skeletal muscle tissue lysate Lane 10: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33147_rabbit-p38-alpha-beta-gamma-mab-psh03-60-28820241430402.jpg "Western Blot: p38 alpha/beta/gamma Antibody (PSH03-60) [NBP3-33147] - Western blot analysis of p38 alpha/beta/gamma on different lysates with Rabbit anti-p38 alpha/beta/gamma antibody (NBP3-33147) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: COS-1 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse skeletal muscle tissue lysate Lane 9: Rat skeletal muscle tissue lysate Lane 10: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")