HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT-2 mouse T cell line. PVDF membrane was probed with 0.05 µg/mL of Goat Anti-Mouse Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1197) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1197-SP | |||
HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Galectin-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1154) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | IC1154G | |||
| Carrier Free | AF1154-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-538-0.025mg | |||
HAF005). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of 4T1 mouse breast cancer cell line, Balb/3T3 mouse embryonic fibroblast cell line and HT-2 mouse T cell line. PVDF membrane was probed with 0.25 µg/mL of Rat Anti-Mouse Galectin-3 Monoclonal Antibody (Catalog # MAB1197) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1197-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1197-GA-050/CF | |||
| Carrier Free | 1197-GA-01M/CF | |||
HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line, MCF-7 human breast cancer cell line, and U-118-MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Galectin-3 Monoclonal Antibody (Catalog # MAB11541) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB11541-SP | |||
| Carrier Free | MAB11541-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | 1154-GA-050/CF | |||
HAF017). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using 
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
1197-GA) was serially diluted and captured by Rat Anti-Human/Mouse/Rat Galectin‑3 Monoclonal Antibody (Catalog # ![N/A Galectin-3 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Galectin3_DGAL30_ELISA_688.jpg "N/A Galectin-3 [HRP]")
![N/A Galectin-3 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_Galectin3_DGAL30_ELISA_689.jpg)
![N/A Galectin-3 [HRP]](http://images.novusbio.com/fullsize/elisa/Galectin-3_DGAL30_ELISA_101.jpg)
042-206). This experiment was conducted under reducing conditions and using the 12-230kDa separation system." class="big_thumb" />
HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
1154-GA) was serially diluted and captured by Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # ![Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Western-Blot-NB300-538-img0017.jpg "Western Blot: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of 25 ug of MCF-7 (Lane 1), Hela (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - LLOMe treatment results in lysosomal damage in T98G glioblastoma cells as evidenced by the accumulation of galectin-3 positive vesicles. T98G cells were treated with LLOMe (1 uM) for 1 h to induce lysosomal damage. Cells were fixed (4% paraformaldehyde) 6 h after washout, blocked with 10% goat serum in 0.1% PBST (1 h, RT), stained with DyLight 488 conjugated galectin-3 antibody (green) (overnight, 4C).The following day, samples were washed with cold PBST, mounted using Prolong Gold mountant with DAPI (blue). Confocal immunofluorescence using DyLight 488 conjugated galectin-3 antibody. This image was submitted via customer Review. Image using the DyLight 488 form of this antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0019.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human colon tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0016.jpg)
![Flow Cytometry: Galectin-3 Antibody (A3A12) [NB300-538] - Using the Alexa Fluor 647 direct conjugate An intracellular stain was performed on HT-29 cells with Galectin-3 (A3A12) antibody NB300-538AF647 (blue) and a matched isotype control NBP1-97005AF647 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Flow-Cytometry-NB300-538-img0018.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of NIH-3T3 cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of rat olfactory bulb](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of MCF-7 cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis of Galectin 3 (green) showing staining in the cytoplasm and nucleus of HeLa cells.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunocytochemistry-Immunofluorescence-NB300-538-img0013.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Mouse colon tissue (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0014.jpg)
![Immunohistochemistry-Paraffin: Galectin-3 Antibody (A3A12) [NB300-538] - Analysis showing positive staining in the nucleus and cytoplasm of Human ovarian carcinoma (right) compared with a negative control in the absence of primary antibody (left).](http://images.novusbio.com/fullsize/Galectin-3-Antibody-A3A12-Immunohistochemistry-Paraffin-NB300-538-img0015.jpg)
![N/A Galectin-3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Galectin3_DY1197_ELISA_1600.jpg "N/A Galectin-3 [Biotin]")
HAF005). A specific band was detected for Galectin-3 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using 
HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />
![A beta oligomerization and Gal-3 expression are age-dependently increased in APP/PS1 mice. a Wild-type (3 months old) and APP/PS1 mice of different ages (3, 5, 8, and 11 months) were sacrificed and dissected hippocampal tissues were subjected to Western blot analysis for endogenous A beta oligomers. b Quantified results for HMW and LMW A beta oligomerization [n = 4 per group; F(4,15) = 39.3, P < 0.001 for HMW and F(4,15) = 74.07, P < 0.001 for LMW]. Statistical significances for various comparisons are shown in the figure. c Western blot analysis showing the expression levels of Gal-3 and PIAS1 in hippocampal samples from the same batch of WT mice and APP/PS1 mice of different ages (n = 4 per group). d Quantified results for Gal-3 expression in WT and APP/PS1 mice [F(4,15) = 14.59, P < 0.001]. e Quantified results for PIAS1 expression in WT and APP/PS1 mice [F(4,15) = 5.86, P < 0.01] The letter “m” indicates month. Statistical significances of various comparisons are shown in the figure. Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, #P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31127200), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab1197_human-mouse-rat-galectin-3-mab-clone-202213-western-blot-121220257492121.jpg)
![A beta oligomerization and Gal-3 expression are increased in AD patients. The frontal lobe lysates of normal subjects and AD patients were subjected to Western blot analysis for A beta oligomerization and the expression of Gal-3 and Gal-1 (n = 4 per group). b–d Quantified results for (b) HMW [t(1,6) = 9.72, P < 0.001] and LMW [t(1,6) = 7.21, P < 0.001] A beta oligomerization, c Gal-3 expression [t(1,6) = 4.92, P < 0.01] and d Gal-1 expression [t(1,6) = 0.43, P > 0.05]. e Immunohistochemical analysis showing the distributions of Gal-3 and ProteoStat staining in normal subjects and AD patients (n = 3 per group). Scale bar, 25 μm. DAPI was used to stain nuclei. Data are expressed as mean ± SEM. **P < 0.01, #P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31127200), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab1197_human-mouse-rat-galectin-3-mab-clone-202213-western-blot-12122025748137.jpg)
![Endogenous Gal-3 expression is decreased but memory performance is improved in APP/PS1;Gal-3+/− mice. a Endogenous expression levels of Gal-3 and NEP were examined by Western blot analysis in 3-month-old WT;WT, APP/PS1;WT, WT;Gal-3−/− and APP/PS1;Gal-3+/− mice. b Quantified results for Gal-3 and NEP expression [n = 4 per group; for Gal-3, F(3,12) = 53.47, P < 0.001; q = 10.43, P < 0.001 for the WT;WT group versus the APP/PS1;WT group; q = 6.46, P < 0.001 for the APP/PS1;WT group versus the APP/PS1;Gal-3+/− group and q = 3.98, P < 0.05 for the WT;WT group versus the APP/PS1;Gal-3+/− group; for NEP, F(3,12) = 27.76, P < 0.001; q = 12.04, P < 0.001 for the WT;WT group versus the APP/PS1;WT group; q = 9.72, P < 0.001 for the WT;WT group versus the WT;Gal-3−/− group and q = 6.29, P < 0.01 for the WT;WT group versus the APP/PS1;Gal-3+/− group]. c Acquisition performance obtained by assessing the water maze learning of 8-month-old mice of the four genotypes [n = 7 per group; F(3,24) = 28.72, P < 0.001; q = 9.33, P < 0.001 for the APP/PS1;WT group versus the WT;WT group and q = 3.59, P < 0.05 for the APP/PS1;Gal-3+/− group versus the APP/PS1;WT group]. d Retention performance of the same mice described in (c) [n = 7 per group; F(3,24) = 4.76, P < 0.01; q = 3.94, P < 0.05 for the APP/PS1;WT group versus the WT;WT group; q = 3.25, P < 0.05 for the APP/PS1;Gal-3+/− group versus the APP/PS1;WT group for the target quadrant]. Representative swim patterns from each group are also shown (upper panel). Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01, #P < 0.001 Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/31127200), licensed under a CC-BY license. Not internally tested by R&D Systems.](http://images.novusbio.com/fullsize/mab1197_human-mouse-rat-galectin-3-mab-clone-202213-western-blot-1212202574378.jpg)
1197-GA) was serially diluted and captured by Rat Anti-Human/Mouse/Rat Galectin‑3 Monoclonal Antibody (Catalog # MAB1197) coated on a Clear Polystyrene Microplate (Catalog # ![N/A Galectin-3 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_Galectin3_DY1154_ELISA_1585.jpg "N/A Galectin-3 [Biotin]")
VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and plasma membrane in epithelial cells. View our protocol for 
1154-GA) was serially diluted and captured by Mouse Anti-Human Galectin‑3 Monoclonal Antibody (Catalog # MAB11541) coated on a Clear Polystyrene Microplate (Catalog # 
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16589] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin-3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16589] - HBL435 xenograft, using Galectin3 antibody at 1:500 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16589-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysates B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0004.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16589] - Galectin-3 antibody detects Galectin-3 protein by western blot analysis. A. 50 ug rat colon lysate/extract. 12% SDS-PAGE. Galectin-3 antibody dilution: 1:10000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16589-img0006.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16589] - Sandwich ELISA detection of recombinant full-length Galectin-3 protein using as capture antibody at concentration of 5 ug/mL and Galectin-3 Antibody as detection antibody at concentration of 1 ug/mL. Rabbit IgG antibody (HRP) was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16589-img0009.jpg)
![<b>Orthogonal Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16589] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-16589_rabbit-polyclonal-galectin-3-antibody-1610202419414598.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0007.jpg "Western Blot: Galectin-3 Antibody [NBP2-16590] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: Galectin-3 Antibody [NBP2-16590] - Immunohistochemical analysis of paraffin-embedded Gastric carcinoma, using antibody at 1:200 dilution.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Immunohistochemistry-Paraffin-NBP2-16590-img0003.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: A549 B: HCT116 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0001.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - Sample (30 ug of whole cell lysate) A: Non-transfected 293T lysate B: LGALS3 transfected 293T lysates 12% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0002.jpg)
![Western Blot: Galectin-3 Antibody [NBP2-16590] - A. 50 ug rat colon lysate/extract 12 % SDS-PAGE Galectin3 antibody dilution: 1:1000](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0004.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Galectin-3 Antibody [NBP2-16590] - Wild-type (WT) and Galectin 3 knownout (KO) HeLa cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with Galectin 3 antibody. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Western-Blot-NBP2-16590-img0005.jpg)
![Sandwich ELISA: Galectin-3 Antibody [NBP2-16590] - Sandwich ELISA detection of recombinant full-length Galectin 3 protein using as detection antibody at concentration of 1 ug/mL. HRP-conjugated rabbit IgG antibody was diluted at 1:10000 and used to detect the primary antibody.](http://images.novusbio.com/fullsize/Galectin-3-Antibody-Sandwich-ELISA-NBP2-16590-img0006.jpg)
