Ifng Products By Gene Name

585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA). The ND₅₀ is typically 0.08-0.2 µg/mL." class="big_lightbox"> 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA). The ND₅₀ is typically 0.08-0.2 µg/mL." alt="Recombinant Rat IFN-gamma (Catalog # 585-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMCV activity elicited by Recombinant Rat IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA). The ND₅₀ is typically 0.08-0.2 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

585-IF) was serially diluted and captured by Mouse Anti-Rat IFN‑ gamma Monoclonal Antibody (Catalog # MAB5851) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 585-IF) was serially diluted and captured by Mouse Anti-Rat IFN‑ gamma Monoclonal Antibody (Catalog # MAB5851) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse/Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-585-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB4851). The ND₅₀ is typically 4-20 ng/mL." class="big_lightbox"> 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB4851). The ND₅₀ is typically 4-20 ng/mL." alt="Recombinant Mouse IFN-gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L-929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN-gamma (2.5 ng/mL) is neutralized (green line) by increasing concentrations of Hamster Anti-Mouse IFN-gamma Monoclonal Antibody (Catalog # MAB4851). The ND₅₀ is typically 4-20 ng/mL." class="big_thumb" />

Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

781-CG) was serially diluted and captured by Mouse Anti-Canine IFN‑ gamma Monoclonal Antibody (Catalog # MAB781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Canine IFN‑ gamma Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox"> 781-CG) was serially diluted and captured by Mouse Anti-Canine IFN‑ gamma Monoclonal Antibody (Catalog # MAB781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Canine IFN‑ gamma Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Canine IFN‑ gamma (Catalog # 781-CG) was serially diluted and captured by Mouse Anti-Canine IFN‑ gamma Monoclonal Antibody (Catalog # MAB781) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Canine IFN‑ gamma Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_lightbox"> 565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND₅₀ is typically 0.3-1.2 µg/mL." alt="Recombinant Cotton Rat IFN-gamma (Catalog # 565-RG) reduces the encephalomyocarditis (EMC)-induced cytopathy in the LCRT cotton rat fibrosarcoma cell line in a dose-dependent manner (orange line), as measured by crystal violet staining. Inhibition of EMC activity elicited by Recombinant Cotton Rat IFN-gamma (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Cotton Rat IFN-gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565). The ND₅₀ is typically 0.3-1.2 µg/mL." class="big_thumb" />

565-RG) was serially diluted and captured by Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 565-RG) was serially diluted and captured by Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

565-RG) was serially diluted and captured by Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 565-RG) was serially diluted and captured by Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Cotton Rat IFN‑ gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF565) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) treated with 50 ng/mL PMA, 1 ug/mL Ionomycin, and 3 uM Monensin overnight were stained with either (A) Mouse Anti-Human IFN- gamma Monoclonal Antibody (Catalog # MAB2851) or (B) Mouse IgG2B Isotype Control (Catalog # MAB0041) followed by anti-Mouse IgG PE-conjugated secondary antibody (Catalog # F0102B) and Mouse Anti-Human CD3 epsilon APC-conjugated Monoclonal Antibody (Catalog # FAB100A). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (5 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB2851). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_lightbox"> 285-IF) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HeLa human cervical epithelial carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Human IFN-gamma (5 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-Human IFN-gamma Monoclonal Antibody (Catalog # MAB2851). The ND₅₀ is typically 0.2-0.6 µg/mL." class="big_thumb" />