| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5832-SP | |||
| Carrier Free | MAB5832-500 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB5831F | |||
| Carrier Free | MAB5831-SP | |||
| Carrier Free | FAB5831V-100UG | |||
| Carrier Free | FAB5831U-100UG | |||
| Carrier Free | FAB5831T-100UG | |||
| Carrier Free | FAB5831S-100UG | |||
| Carrier Free | FAB5831R-100UG | |||
| Carrier Free | FAB5831N-100UG | |||
| Carrier Free | FAB5831G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90585-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50431-0.025ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48214 | |||
| Azide and BSA Free | NBP3-11490 | |||
![N/A ICAM-1/CD54 [Biotin]](http://images.novusbio.com/fullsize2/DATA_ICAM1_DY583_ELISA_2135.jpg "N/A ICAM-1/CD54 [Biotin]")
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0005.jpg "Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1/CD54 on Daudi cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R))488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0003.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Flow cytometric analysis of ICAM-1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (M1511-6, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a FITC-conjugated Goat anti-Mouse IgG Secondary antibody at 1/200 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Flow-Cytometry-NBP2-66896-img0004.jpg)
![Western Blot: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - Western blot analysis of ICAM-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M1511-6, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.Positive control:Lane 1: Raji cell lysateLane 2: Daudi cell lysate](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Western-Blot-NBP2-66896-img0001.jpg)
![Immunocytochemistry: ICAM-1/CD54 Antibody (J0-F7) [NBP2-66896] - ICC staining of ICAM-1 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (M1511-6, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor(R)488 Goat anti-Mouse IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue)](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-J0-F7-Immunocytochemistry-NBP2-66896-img0002.jpg)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0002.jpg "Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse kidney with rabbit monoclonal antibody (1:200). Positive staining was localized to blood vessel.")
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (020) [NBP2-90586] - Staining of mouse ICAM-1/CD54 in mouse brain with rabbit monoclonal antibody (1:200). The image showing positive staining of choroid plexus.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-020-Immunohistochemistry-Paraffin-NBP2-90586-img0001.jpg)
![Flow Cytometry: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Flow cytometric analysis of Mouse ICAM-1/CD54 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM-1/CD54, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-009-Flow-Cytometry-NBP2-90585-img0001.jpg "Flow Cytometry: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Flow cytometric analysis of Mouse ICAM-1/CD54 expression on BABL/c splenocytes. Cells were stained with purified anti-Mouse ICAM-1/CD54, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.")
![Immunocytochemistry/Immunofluorescence: ICAM-1/CD54 Antibody (009) [NBP2-90585] - Immunofluorescence staining of Mouse ICAM-1/CD54 in Raw264.7 cells. Cells were fixed with 4% PFA, blocked with 10% serum, and incubated with NBP2-90585 antibody (dilution ratio 1:60) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green). Positive staining was localized to Cytoplasm and Cell membrane.](http://images.novusbio.com/fullsize/nbp2-90585_rabbit-monoclonal-icam-1-cd54-antibody-009-23520252135170.png)
![Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (W-CAM-1 (same as Wehi-CAM-1 or 1H4)) [NBP2-44331] - Human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-W-CAM-1-same-as-Wehi-CAM-1-or-1H4-Immunohistochemistry-Paraffin-NBP2-44331-img0001.jpg "Immunohistochemistry-Paraffin: ICAM-1/CD54 Antibody (W-CAM-1 (same as Wehi-CAM-1 or 1H4)) [NBP2-44331] - Human Melanoma stained with CD54 Monoclonal Antibody (W-CAM-1).")
![Sandwich ELISA: Monkey ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-92712] - Representative standard curve for monkey ICAM-1 ELISA. Monkey ICAM-1 was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.](http://images.novusbio.com/fullsize/Primate-ICAM-1-CD54-ELISA-Kit-Colorimetric-Sandwich-ELISA-NBP1-92712-img0001.jpg "Sandwich ELISA: Monkey ICAM-1/CD54 ELISA Kit (Colorimetric) [NBP1-92712] - Representative standard curve for monkey ICAM-1 ELISA. Monkey ICAM-1 was diluted in serial 2-fold steps in Sample Diluent. Do not use this standard curve to derive test results. A standard curve must be run for each group of microwell strips assayed.")
![Western Blot: ICAM1 Overexpression Lysate (Adult Normal) [NBL1-11804] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM1.](http://images.novusbio.com/fullsize/ICAM1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11804-img0002.jpg "Western Blot: ICAM1 Overexpression Lysate (Adult Normal) [NBL1-11804] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ICAM1.")
