| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-1798SS | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03393-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55039-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-49576-25ul | |||
![Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western blotting using IRP2 or b-actin antibodies diluted 1:500 in TBS-Tween.](http://images.novusbio.com/fullsize/IRP2-Antibody-Western-Blot-NB100-1798-img0008.jpg "<b>Biological Strategies Validation. </b>Western Blot: IRP2 Antibody [NB100-1798] - Raw macrophages (mouse) were either left untreated (lane 1) or treated overnight with 100mM DFO (lane 2) or 100mM hemin (lane 3). Proteins extracts were subjected to western blotting using IRP2 or b-actin antibodies diluted 1:500 in TBS-Tween.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: IRP2 Antibody [NB100-1798] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human placenta using 1:300 conc. of IRP2 antibody on a Bond Rx autostainer (Leica Biosystems). The assay involved 30 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 9.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 15 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Cytoplasmic staining was observed in villi of endothelial cells.](http://images.novusbio.com/fullsize/IRP2-Antibody-Immunohistochemistry-Paraffin-NB100-1798-img0009.jpg)
![Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in mouse liver lysate.](http://images.novusbio.com/fullsize/IRP2-Antibody-Western-Blot-NB100-1798-img0004.jpg)
![Western Blot: IRP2 Antibody [NB100-1798] - Detection of IRP2 in RAW 264.7 lysate using NB100-1798. Lane 1: Lot B Lane 2: Lot C](http://images.novusbio.com/fullsize/IRP2-Antibody-Western-Blot-NB100-1798-img0007.jpg)
![Western Blot: IRP2 Antibody [NBP3-03393] - Western blot analysis of extracts of various cell lines, using IRP2 antibody (NBP3-03393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.](http://images.novusbio.com/fullsize/IRP2 Antibody-Western Blot-NBP3-03393-img0002.jpg "Western Blot: IRP2 Antibody [NBP3-03393] - Western blot analysis of extracts of various cell lines, using IRP2 antibody (NBP3-03393) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.")
![Immunocytochemistry/ Immunofluorescence: IRP2 Antibody - Azide and BSA Free [IRP2] - Immunofluorescence analysis of A549 cells using IRP2 Rabbit pAb . Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp3-03393_rabbit-polyclonal-irp2-antibody-52202516321497.jpg)
![SDS-Page: Recombinant Human IRP2 Protein [H00003658-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00003658-P01-1.jpg "SDS-Page: Recombinant Human IRP2 Protein [H00003658-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Mouse liver tissue lysate (40 ug/Lane) Lane 4: Mouse kidney tissue lysate (40 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32483_mouse-irp2-mab-9d1-r-27820241631635.jpg "Western Blot: IRP2 Antibody (9D1-R) [NBP3-32483] - Western blot analysis of IRP2 on different lysates with Mouse anti-IRP2 antibody (NBP3-32483) at 1/2,000 dilution. Lane 1: Daudi cell lysate (20 ug/Lane) Lane 2: A549 cell lysate (20 ug/Lane) Lane 3: Mouse liver tissue lysate (40 ug/Lane) Lane 4: Mouse kidney tissue lysate (40 ug/Lane) Lane 5: Mouse heart tissue lysate (40 ug/Lane) Lane 6: Rat liver tissue lysate (40 ug/Lane) Predicted band size: 105 kDa Observed band size: 105 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32483) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight. Anti-Mouse IgG for IP Nano-secondary antibody at 1/5,000 dilution was used for 1 hour at room temperature.")
![Western Blot: IRP2 Antibody [NBP3-10538] - Host: Rabbit. Target Name: IRP2. Sample Tissue: Human Fetal Lung. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize/IRP2 Antibody-Western Blot-NBP3-10538-img0001.jpg "Western Blot: IRP2 Antibody [NBP3-10538] - Host: Rabbit. Target Name: IRP2. Sample Tissue: Human Fetal Lung. Antibody Dilution: 1.0ug/ml")
![Immunocytochemistry/Immunofluorescence: IRP2 Antibody [NBP2-55039] - Staining of human cell line HEK 293 shows localization to cell junctions.](http://images.novusbio.com/fullsize/IRP2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55039-img0001.jpg "Immunocytochemistry/Immunofluorescence: IRP2 Antibody [NBP2-55039] - Staining of human cell line HEK 293 shows localization to cell junctions.")
![Immunohistochemistry-Paraffin: IRP2 Antibody [NBP2-49576] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize/IRP2-Antibody-Immunohistochemistry-Paraffin-NBP2-49576-img0003.jpg "Immunohistochemistry-Paraffin: IRP2 Antibody [NBP2-49576] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.")
![Western Blot: IRP2 Overexpression Lysate (Adult Normal) [NBL1-12029] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRP2.](http://images.novusbio.com/fullsize/IRP2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-12029-img0002.jpg "Western Blot: IRP2 Overexpression Lysate (Adult Normal) [NBL1-12029] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for IRP2.")
![ELISA: Human IRP2 ELISA Kit (Colorimetric) [NBP3-39631] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39631_human-irp2-elisa-kit-colorimetric-2110202415403135.png "ELISA: Human IRP2 ELISA Kit (Colorimetric) [NBP3-39631] - Standard Curve Reference")
![ELISA: Human IRP2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39632] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39632_human-irp2-ready-to-use-elisa-kit-colorimetric-211020241536232.png "ELISA: Human IRP2 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39632] - Standard Curve Reference")