IL22 Products By Gene Name

MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules." alt="Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

DY217B). IL-10 Secretion elicited by Recombinant Mouse IL-22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB5821). The ND₅₀ is typically 0.5-6 µg/mL." class="big_lightbox"> DY217B). IL-10 Secretion elicited by Recombinant Mouse IL-22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB5821). The ND₅₀ is typically 0.5-6 µg/mL." alt="Recombinant Mouse IL-22 stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 Secretion elicited by Recombinant Mouse IL-22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB5821). The ND₅₀ is typically 0.5-6 µg/mL." class="big_thumb" />