IL-1 RII: Novus Biologicals

DR1B00

Human IL-1 RII Quantikine ELISA Kit

(16 Publications)

1 Kit

DR1B00

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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MAB263

IL-1 RII Antibody (32437) [Unconjugated]

(3 Publications)

500 μg

MAB263

263-2R) inhibits Recombinant Human IL-1 beta / IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND₅₀ is typically 2-6 µg/mL." class="big_lightbox">

263-2R) inhibits Recombinant Human IL-1 beta / IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND₅₀ is typically 2-6 µg/mL." alt="Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta / IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB263). The ND₅₀ is typically 2-6 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

IL‑1 RII was detected in immersion fixed HDLM‑2 human Hodgkin’s lymphoma cells (positive) and absent in K562 human chronic myelogenous leukemia cells (negative) using Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB263) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

HDLM-2 cells were stained with Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB263, filled histogram) or isotype control antibody (Catalog # <a class=

MAB003, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

$Isolation of SP cells from human first trimester vCTB.(A) Immunocytochemistry images of CGB, CK7, CSH1, HLA−G, CD9 and HLA1 expression in primary vCTB cells isolated by Percoll gradient centrifugation. Each protein expression is shown as red signals (scale bars, 50 µm). The isolated cells were plated on chamber slides with HBM and stained the next day. (B) The SP cells represent 0.12±0.28% (n = 22) of the total primary vCTB. Co-incubation of the cells with verapamil erased the SP cell fraction. (C) The expression of CD105, CD146 and VIM in SP cells from primary vCTB cells was analyzed by immunocytochemistry. All images show counterstaining with Hoechst 33852. Each protein expression is shown as red signals (scale bars, 50 µm). (D) Immunocytochemistry images of IL7R and IL1R2 expression in SP and NSP cells counterstained with Hoechst 33852. IL7R and IL1R2 expressions are shown as red signals (scale bars, 50 µm). (E) The expression of CGB, CSH1 HLA−G, CK7, PHLDA2 and EOMES in IL7R/IL1R2 double-positive cells was assessed by immunocytochemistry. These expressions are shown as green signals (scale bars, 50 µm). (F) Induction of differentiation in IL7R/IL1R2 positive cells was determined by IL7R, IL1R2, CSH1 and CGB expression. IL7R/IL1R2 positive cells from primary human vCTB were analyzed after being cultured in HBM for 2 weeks. IL7R and IL1R2 expressions are shown as red signals; CSH1 and CGB expressions are shown as green signals (scale bars, 50 µm). The cell marked with an arrow show positive for CGB and negative for IL7R expression. In contrast, the cell marked with an arrowhead was positive for IL7R and negative for CGB expression. The outlines of the cells are indicated by broken lines. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21760941), licensed under a CC-BY license. Not internally tested by R&D Systems.$

IL7R and IL1R2 were abundantly expressed in HTR-8/SVneo-SP cells.(A) IL7R and IL1R2 mRNA expressions between HTR-8/SVneo-SP and -NSP cells were compared by real-time RT-PCR. The mRNA levels were normalized to those of GAPDH and error bars show standard deviations (n = 3). Asterisk indicates statistically significant (p<0.01). (B) Western blot analysis of IL7R, IL1R2 and CXCR7 proteins in HTR-8/SVneo-SP and -NSP cells. SP cells exclusively expressed IL7R and IL1R2, while NSP exclusively expressed CXCR7. (C) The protein expression of IL7R, IL1R2 or CXCR7 was analyzed by immunocytochemistry. All images counterstained with Hoechst 33852. Each protein expression is shown as green signals (scale bars, 50 µm). (D) IL7R and IL1R2 expressions in HTR-8/SVneo-SP cells were analyzed by immunocytochemistry. All images were counterstained with Hoechst 33852. IL7R and IL1R2 expressions are shown as red and green signals respectively (scale bars, 50 µm). Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21760941), licensed under a CC-BY license. Not internally tested by R&D Systems.

SP and IL1R2/IL7R double-positive cells differentiated into multiple trophoblast cell lineages.(A) Immunocytochemistry images of IL7R, IL1R2, CK7, CSH1, CGB and HLA−G expression in SP cells sorted from HTR-8/SVneo parent cells. Each protein expression is shown as green signals (scale bars, 50 µm). The cells were assayed after culture for 2 weeks in HSM or HBM. (B) Flow cytometry results of IL1R2-positive cells after being sorted by MACS. SP cells were obtained from IL1R2-positive cells after culture for 2 weeks (4.78±1.45% from 3 independent experiments). (C) Induction of trophoblast differentiation of IL7R/IL1R2 double-positive HTR-8/SVneo cells confirmed by differentiation marker, CSH1 and CGB expression. IL7R and IL1R2 expressions are shown as red signals; CSH1 and CGB expressions are shown as green signals (scale bars, 50 µm). The cells marked with an arrow show positive for CSH1 or CGB and negative for IL1R2 or IL7R expression. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/21760941), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB263U-100UG
Alexa Fluor 405	FAB263V-100UG
Alexa Fluor 488	FAB263G-100UG
Alexa Fluor 532	FAB263X-100UG
Alexa Fluor 594	FAB263T-100UG
Alexa Fluor 647	FAB263R-100UG
Alexa Fluor 700	FAB263N-100UG
Alexa Fluor 750	FAB263S-100UG
Alexa Fluor Plus 405	FAB263AFP405-100UG
Alexa Fluor Plus 488	FAB263AFP488-100UG
Alexa Fluor Plus 555	FAB263AFP555-100UG
Alexa Fluor Plus 594	FAB263AFP594-100UG
Alexa Fluor Plus 647	FAB263AFP647-100UG
Alexa Fluor Plus 680	FAB263AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB263-SP

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NBP1-32681

IL-1 RII Antibody - BSA Free

(2 Publications)

100 ul

NBP1-32681

IL-1-RII-Antibody-Immunohistochemistry-NBP1-32681-img0013.jpg

IL-1-RII-Antibody-Chromatin-Immunoprecipitation-NBP1-32681-img0014.jpg

Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Human lung cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Immunohistochemistry-Paraffin: IL-1 RII Antibody [NBP1-32681] - Human colon cancer. IL1 Receptor 2 antibody [N3C3] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

<b>Orthogonal Strategies Validation. </b>LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL-1 RII antibody [N3C3] (NBP1-32681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

LoVo whole cell and membrane extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IL1 Receptor 2 antibody [N3C3] (NBP1-32681) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Reactivity:

Hu, Mu, Rt, RM

Species Glossary

Reactivity: Hu, Mu, Rt, RM

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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663-2R

Recombinant Human IL-1 RII Fc Chimera Protein, CF

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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AF563

IL-1 RII Antibody [Unconjugated]

(4 Publications)

100 μg

AF563

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_lightbox">

AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." alt="EL-4 mouse lymphoblast cell line was stained with Goat Anti-Mouse IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF563, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0108)." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox">

IL-1 RII was detected in perfusion fixed frozen sections of mouse spleen using Goat Anti-Mouse IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF563) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF563U-100UG
Alexa Fluor 405	AF563V-100UG
Alexa Fluor 532	AF563X-100UG
Alexa Fluor 594	AF563T-100UG
Alexa Fluor 647	AF563R-100UG
Alexa Fluor 700	AF563N-100UG
Alexa Fluor 750	AF563S-100UG
Alexa Fluor Plus 405	AF563AFP405-100UG
Alexa Fluor Plus 488	AF563AFP488-100UG
Alexa Fluor Plus 555	AF563AFP555-100UG
Alexa Fluor Plus 594	AF563AFP594-100UG
Alexa Fluor Plus 647	AF563AFP647-100UG
Alexa Fluor Plus 680	AF563AFP680-100UG
Allophycocyanin	AF563A
Biotin	BAF563
CoraFluor 1	AF563CL1
DyLight 350	AF563D
DyLight 405	AF563E
DyLight 488	AF563K
DyLight 550	AF563L
DyLight 594	AF563M
DyLight 650	AF563W
DyLight 680	AF563Y
DyLight 755	AF563Z
FITC	AF563F
HRP	AF563H
Janelia Fluor 525	AF563JF525
Janelia Fluor 549	AF563I
Janelia Fluor 646	AF563J
Janelia Fluor 669	AF563JF669
PE	AF563P
PerCP	AF563C
mFluor Violet 450 SE	AF563MFV450
mFluor Violet 500 SE	AF563MFV500
mFluor Violet 610 SE	AF563MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF563-SP

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AF-263-NA

IL-1 RII Antibody [Unconjugated]

(6 Publications)

100 μg

AF-263-NA

263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND₅₀ is typically 15‑30 µg/mL." class="big_lightbox">

263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND₅₀ is typically 15‑30 µg/mL." alt="Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA). The ND₅₀ is typically 15‑30 µg/mL." class="big_thumb" />

263-2R) was serially diluted and captured by Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB663) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human IL‑1 RII (Catalog # <a class=

263-2R) was serially diluted and captured by Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB663) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-263-NAU-100UG
Alexa Fluor 405	AF-263-NAV-100UG
Alexa Fluor 488	AF-263-NAG-100UG
Alexa Fluor 532	AF-263-NAX-100UG
Alexa Fluor 594	AF-263-NAT-100UG
Alexa Fluor 647	AF-263-NAR-100UG
Alexa Fluor 700	AF-263-NAN-100UG
Alexa Fluor 750	AF-263-NAS-100UG
Alexa Fluor Plus 405	AF-263-NAAFP405
Alexa Fluor Plus 488	AF-263-NAAFP488
Alexa Fluor Plus 555	AF-263-NAAFP555
Alexa Fluor Plus 594	AF-263-NAAFP594
Alexa Fluor Plus 647	AF-263-NAAFP647
Alexa Fluor Plus 680	AF-263-NAAFP680
Allophycocyanin	AF-263-NAA
Biotin	BAF263
CoraFluor 1	AF-263-NACL1
DyLight 350	AF-263-NAD
DyLight 405	AF-263-NAE
DyLight 488	AF-263-NAK
DyLight 550	AF-263-NAL
DyLight 594	AF-263-NAM
DyLight 650	AF-263-NAW
DyLight 680	AF-263-NAY
DyLight 755	AF-263-NAZ
FITC	AF-263-NAF
HRP	AF-263-NAH
Janelia Fluor 525	AF-263-NAJF525
Janelia Fluor 549	AF-263-NAI
Janelia Fluor 646	AF-263-NAJ
Janelia Fluor 669	AF-263-NAJF669
PE	AF-263-NAP
PerCP	AF-263-NAC
mFluor Violet 450 SE	AF-263-NAMFV450
mFluor Violet 500 SE	AF-263-NAMFV500
mFluor Violet 610 SE	AF-263-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-263-SP

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MAB663

IL-1 RII Antibody (34141) [Unconjugated]

(1 Publication)

100 μg

MAB663

263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 RII Monoclonal Antibody (Catalog # MAB663). The ND₅₀ is typically 5-20 µg/mL." class="big_lightbox">

263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 RII Monoclonal Antibody (Catalog # MAB663). The ND₅₀ is typically 5-20 µg/mL." alt="Recombinant Human IL-1 RII (263-2R) inhibits Recombinant Human IL-1 beta /IL-1F2 (201-LB) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 beta /IL-1F2 (50 pg/mL) activity elicited by Recombinant Human IL-1 RII (2 µg/mL) is neutralized (green line) by increasing concentrations of Human IL-1 RII Monoclonal Antibody (Catalog # MAB663). The ND₅₀ is typically 5-20 µg/mL." class="big_thumb" />

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_lightbox">

HDLM-2 cells were stained with Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB663, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />

263-2R) was serially diluted and captured by Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB663) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

263-2R) was serially diluted and captured by Mouse Anti-Human IL‑1 RII Monoclonal Antibody (Catalog # MAB663) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑1 RII Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-263-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB663U
Alexa Fluor 405	FAB663V
Alexa Fluor 488	FAB663G
Alexa Fluor 532	FAB663X
Alexa Fluor 594	FAB663T
Alexa Fluor 647	FAB663R
Alexa Fluor 700	FAB663N
Alexa Fluor 750	FAB663S
Allophycocyanin	FAB663A
Biotin	FAB663B
CoraFluor 1	FAB663CL1
DyLight 350	FAB663D
DyLight 405	FAB663E
DyLight 488	FAB663K
DyLight 550	FAB663L
DyLight 594	FAB663M
DyLight 650	FAB663W
DyLight 680	FAB663Y
DyLight 755	FAB663Z
Fluorescein	FAB663F
HRP	FAB663H
Janelia Fluor 525	FAB663JF525
Janelia Fluor 549	FAB663I
Janelia Fluor 646	FAB663J
Janelia Fluor 669	FAB663JF669
Phycoerythrin	FAB663P
mFluor Violet 450 SE	FAB663MFV450
mFluor Violet 500 SE	FAB663MFV500
mFluor Violet 610 SE	FAB663MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	FAB663F
Carrier Free	FAB663P
Carrier Free	FAB663A
Carrier Free	FAB663N
Carrier Free	MAB663-SP
Carrier Free	MAB663-500

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263-2R

Recombinant Human IL-1 RII Protein

(4 Publications)

50 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	263-2R-050/CF

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DY263

Human IL-1 RII DuoSet ELISA, 15 Plate

(3 Publications)

1 Kit (for 15 Plates)

DY263

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

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563-MR

Recombinant Mouse IL-1 RII Fc Chimera Protein, CF

(3 Publications)

100 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

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MAB2631

IL-1 RII Antibody (34117) [Unconjugated]

500 μg

MAB2631

HAF007). A specific band was detected for IL-1 RII at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

<b>Biological Strategies Validation. </b>Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB2631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF007). A specific band was detected for IL-1 RII at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human peripheral blood mononuclear cells (PBMC) untreated (-) or treated (+) with 1 µg/mL PHA for 5 days. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human IL-1 RII Monoclonal Antibody (Catalog # MAB2631) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-1 RII at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB2631U-100UG
Alexa Fluor 405	FAB2631V-100UG
Alexa Fluor 488	FAB2631G-100UG
Alexa Fluor 532	FAB2631X-100UG
Alexa Fluor 594	FAB2631T-100UG
Alexa Fluor 647	FAB2631R-100UG
Alexa Fluor 700	FAB2631N-100UG
Alexa Fluor 750	FAB2631S-100UG
Alexa Fluor Plus 405	FAB2631AFP405-100UG
Alexa Fluor Plus 488	FAB2631AFP488-100UG
Alexa Fluor Plus 555	FAB2631AFP555-100UG
Alexa Fluor Plus 594	FAB2631AFP594-100UG
Alexa Fluor Plus 647	FAB2631AFP647-100UG
Alexa Fluor Plus 680	FAB2631AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB2631-SP

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MAB563

IL-1 RII Antibody (130817)

(1 Publication)

500 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB563U-100UG
Alexa Fluor 405	FAB563V-100UG
Alexa Fluor 532	FAB563X-100UG
Alexa Fluor 594	FAB563T-100UG
Alexa Fluor 647	FAB563R-100UG
Alexa Fluor 700	FAB563N-100UG
Alexa Fluor 750	FAB563S-100UG
Alexa Fluor Plus 405	FAB563AFP405-100UG
Alexa Fluor Plus 488	FAB563AFP488-100UG
Alexa Fluor Plus 555	FAB563AFP555-100UG
Alexa Fluor Plus 594	FAB563AFP594-100UG
Alexa Fluor Plus 647	FAB563AFP647-100UG
Alexa Fluor Plus 680	FAB563AFP680-100UG

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB563-SP

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Host

Available Conjugates

Format

Modification

Clonality

Clone

Gene Symbol

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

IL1R2 Products By Gene Name

Human IL-1 RII Quantikine ELISA Kit

IL-1 RII Antibody (32437) [Unconjugated]

IL-1 RII Antibody - BSA Free

Recombinant Human IL-1 RII Fc Chimera Protein, CF

IL-1 RII Antibody [Unconjugated]

IL-1 RII Antibody [Unconjugated]

IL-1 RII Antibody (34141) [Unconjugated]

Recombinant Human IL-1 RII Protein

Human IL-1 RII DuoSet ELISA, 15 Plate

Recombinant Mouse IL-1 RII Fc Chimera Protein, CF

IL-1 RII Antibody (34117) [Unconjugated]

IL-1 RII Antibody (130817)