IL10 Products By Gene Name

HAF008). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF008). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB91842) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for IL-10 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in enteroendocrine cells of duodenum. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox"> VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in enteroendocrine cells of duodenum. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />

735-CL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑10 Monoclonal Antibody (Catalog # MAB7352). The ND₅₀ is typically 0.1-0.25 µg/mL." class="big_lightbox"> 735-CL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑10 Monoclonal Antibody (Catalog # MAB7352). The ND₅₀ is typically 0.1-0.25 µg/mL." alt="Recombinant Canine IL‑10 (Catalog # 735-CL) stimulates proliferation in the MC/9‑2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL‑10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL‑10 Monoclonal Antibody (Catalog # MAB7352). The ND₅₀ is typically 0.1-0.25 µg/mL." class="big_thumb" />

F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_lightbox"> F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." alt="Human peripheral blood mononuclear cells (PBMCs) either (A) stimulated to induce Th2 cells or (B) unstimulated were stained with Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210) followed by Fluorescein-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0112) and Mouse Anti-Human CD4 Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # FAB3791N). Quadrant markers were set based on control antibody staining (Catalog # AB-105-C). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules." class="big_thumb" />

217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210). The ND₅₀ is typically 75-450 ng/mL." class="big_lightbox"> 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB9210). The ND₅₀ is typically 75-450 ng/mL." class="big_thumb" />

915-VL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Viral EBV IL-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral EBV IL-10 Monoclonal Antibody (Catalog # MAB915). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_lightbox"> 915-VL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Viral EBV IL-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral EBV IL-10 Monoclonal Antibody (Catalog # MAB915). The ND₅₀ is typically 0.1-0.4 µg/mL." alt="Recombinant Viral EBV IL-10 (Catalog # 915-VL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Viral EBV IL-10 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Viral EBV IL-10 Monoclonal Antibody (Catalog # MAB915). The ND₅₀ is typically 0.1-0.4 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-10 was detected in immersion fixed porcine peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Porcine IL-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF693) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />