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![SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.](http://images.novusbio.com/fullsize/Sheep-IgM-Isotype-Control-SDS-Page-NBP1-96774-img0001.jpg "SDS-Page: Sheep IgM Isotype Control [NBP1-96774] - Lane 1: Sheep IgM, Non-Reduced. Lane 2: Sheep IgM, Reduced. Load: 1.0 ug per lane.")
![SDS-Page: Porcine IgM Isotype Control [NBP1-96788] - Lane 1: Swine IgM, 1.0 ug, Non-reduced. Lane 2: Swine IgM, 5.0 ug, Non-reduced. Lane 3: Swine IgM, 10.0 ug, Non-reduced. M: Opal Pre-stained Marker. Lane 4: Swine IgM, 1.0 ug, Reduced. Lane 5: Swine IgM, 5.0 ug, Reduced. Lane 6: Swine IgM, 10.0 ug, Reduced. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.](http://images.novusbio.com/fullsize/Porcine-IgM-Isotype-Control-SDS-Page-NBP1-96788-img0001.jpg "SDS-Page: Porcine IgM Isotype Control [NBP1-96788] - Lane 1: Swine IgM, 1.0 ug, Non-reduced. Lane 2: Swine IgM, 5.0 ug, Non-reduced. Lane 3: Swine IgM, 10.0 ug, Non-reduced. M: Opal Pre-stained Marker. Lane 4: Swine IgM, 1.0 ug, Reduced. Lane 5: Swine IgM, 5.0 ug, Reduced. Lane 6: Swine IgM, 10.0 ug, Reduced. Predicted/Observed size - Non-Reduced: 900 kDa, Reduced - 75, 25 kDa.")
![SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.](http://images.novusbio.com/fullsize/Rat-IgM-Isotype-Control-SDS-Page-NBP1-96776-img0001.jpg "SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane M: 3 uL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment. Lane 3: Reduced Rat IgG Fab Fragment. Lane 4: Reduced Rat IgM Whole Molecule. Load: 1 ug of IgG, F(c), Fab; 1.5 ug of IgM.")
![SDS-Page: Rat IgM Isotype Control [NBP1-96776] - Lane 1: Rat IgM, Non-Reduced. Lane 2: Rat IgM, Reduced. Load: 1.0 ug per lane.](http://images.novusbio.com/fullsize/Rat-IgM-Isotype-Control-SDS-Page-NBP1-96776-img0002.jpg)
![Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-Dot-Blot-NBP1-72789-img0001.jpg "Dot Blot: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - Mouse IgM. Load: Lane 1 - 200 ng Lane 2 - 66.7 ng Lane 3 - 22.2 ng Lane 4 - 7.41 ng Lane 5 - 2.47 ng.")
![ELISA: Rabbit anti-Mouse IgM Heavy Chain Secondary Antibody [NBP1-72789] - ELISA Results of Rabbit anti-Mouse IgM Heavy Chain Secondary antibody tested against purified Mouse IgM. Each well was coated in duplicate with 10 ug of Mouse IgM. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel/PBS Blocking buffer, Goat Anti-Rabbit IgG mx10 HRP conjugated, and TMB substrate.](http://images.novusbio.com/fullsize/Rabbit-anti-Mouse-IgM-Heavy-Chain-Secondary-Antibody-ELISA-NBP1-72789-img0004.jpg)
![Western Blot: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. Lane 4: Chicken IgM Whole Molecule. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher. Image using the Biotin form of this antibody.](http://images.novusbio.com/fullsize/Goat-anti-Chicken-IgM-Heavy-Chain-Secondary-Antibody-Western-Blot-NBP2-60690-img0001.jpg "Western Blot: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Chicken IgG whole molecule. Lane 2: Chicken IgG F(c) Fragment. Lane 3: Chicken IgG F(ab) Fragment. Lane 4: Chicken IgM Whole Molecule. All samples were reduced. Load: 50 ng per lane. Block: incubated with blocking buffer for 30 min at RT. Primary Antibody: Anti-Chicken IgM (mu chain) (GOAT) Antibody Peroxidase Conjugated 1:30,000 for 60 min at RT. Predicted/Observed Size: 25 and 72 kDa for Chicken IgG, 25 kDa for F(c) and Fab, 75 kDa for IgM. Chicken F(c) migrates slightly higher. Image using the Biotin form of this antibody.")
![SDS-Page: Goat anti-Chicken IgM Heavy Chain Secondary Antibody [NBP2-60690] - Lane 1: Goat anti-Chicken IgM Secondary Antibody - Non-Reduced. Lane 2: Goat anti-Chicken IgM Secondary Antibody - Reduced. Load: 1.0 ug per lane. Predicted/Observed size: 50 kDa, 25 kDa for Reduced - Goat IgG. Other band(s): none.](http://images.novusbio.com/fullsize/Goat-anti-Chicken-IgM-Heavy-Chain-Secondary-Antibody-SDS-Page-NBP2-60690-img0002.jpg)