| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21687-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26028 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26027 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26029 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98972-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-15983-20ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26029 | |||
![Immunohistochemistry: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21687.](http://images.novusbio.com/fullsize/nbp3-21687_rabbit-histone-h2b-type-1b-mab-sr1212-57202316582125.jpg "Immunohistochemistry: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] - Immunohistochemical analysis of paraffin-embedded human colon, using NBP3-21687.")
![Western Blot: Histone H2B type 1B Antibody (SR1212) [NBP3-21687] - Western blot analysis using NBP3-21687 in Hela cell treated with TSA lysate.](http://images.novusbio.com/fullsize/nbp3-21687_rabbit-histone-h2b-type-1b-mab-sr1212-572023152469.jpg)
![Western Blot: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30 minutes. All lanes: Histone H2B type 1B antibody at 0.135ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa. Observed band size: 15 KDa.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Western-Blot-NBP3-15229-img0004.jpg "Western Blot: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Positive WB detected in Hela whole cell lysate treated by 15mM sodium butyrate for 30 minutes. All lanes: Histone H2B type 1B antibody at 0.135ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 15 KDa. Observed band size: 15 KDa.")
![Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Immunofluorescence staining of Hela cells (treated by 15mM sodium butyrate for 30 minutes) with at Histone H2B type 1B antibody 1:84, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Immunocytochemistry-Immunofluorescence-NBP3-15229-img0003.jpg)
![Flow Cytometry: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Overlay histogram showing Hela cells stained with Histone H2B type 1B antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4C.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4C. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Flow-Cytometry-NBP3-15229-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (21F11) [NBP3-15229] - Immunocytochemistry analysis of Histone H2B type 1B antibody diluted at 1:100 and staining in Hela cells. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-21F11-Immunocytochemistry-Immunofluorescence-NBP3-15229-img0002.jpg)
![Western Blot: Histone H2B type 1B [ac Lys23] Antibody (RM260) [NBP2-61500] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using NBP2-61500 at 0.5 ug/ml](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys23-Antibody-RM260-Western-Blot-NBP2-61500-img0004.jpg "Western Blot: Histone H2B type 1B [ac Lys23] Antibody (RM260) [NBP2-61500] - Western Blot of (1) acid extracts of HeLa cells treated with sodium butyrate, (2) acid extracts of HeLa cells untreated, and (3) recombinant Histone H2B, using NBP2-61500 at 0.5 ug/ml")
![Multiplex Immunoassay: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP2-61500] - RM260 specifically reacts to Histone H2B acetylated at Lysine 23 (K23ac). No cross reactivity with acetylated Lysine 5 (K5ac), Lysine 11 (K11ac), Lysine 12 (K12ac), Lysine 15 (K15ac), Lysine 20 (K20ac), or non-mordified Lysine 23 in histone H2B.](http://images.novusbio.com/fullsize/nbp2-61500_rabbit-monoclonal-histone-h2b-type-1b-ac-lys23-antibody-rm260-multiplex-immunoassay-189202523403512.png)
![Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys23] Antibody (RM260) - Azide and BSA Free [NBP2-61500] - Immunocytochemistry of HeLa cells treated with sodium butyrate, using Clone RM260 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61500_rabbit-monoclonal-histone-h2b-type-1b-ac-lys23-antibody-rm260-immunocytochemistry-immunofluorescence-189202523362017.png)
![Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61496 at 0.5 ug/ml](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-ac-Lys20-Antibody-RM235-Western-Blot-NBP2-61496-img0004.jpg "<b>Biological Strategies Validation. </b>Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61496 at 0.5 ug/ml")
![<b>Biological Strategies Validation. </b>Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP2-61496] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using Clone RM235 (red). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61496_rabbit-monoclonal-histone-h2b-type-1b-ac-lys20-antibody-rm235-immunocytochemistry-immunofluorescence-189202523423514.png)
![Multiplex Immunoassay: Histone H2B type 1B [ac Lys20] Antibody (RM235) - Azide and BSA Free [NBP2-61496] - RM235 specifically reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.](http://images.novusbio.com/fullsize/nbp2-61496_rabbit-monoclonal-histone-h2b-type-1b-ac-lys20-antibody-rm235-multiplex-immunoassay-18920252352117.png)
![Immunocytochemistry/Immunofluorescence: Histone H2B type 1B Antibody [NBP3-23376] - Immunocytochemical labeling of Histone H2B type 1B in methanol and acetone fixed rat A7r5 cells. The cells were labeled with rabbit polyclonal Histone H2B type 1B (C-terminus) antibody (NBP3-23376), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.](http://images.novusbio.com/fullsize/nbp3-23376_rabbit-histone-h2b-type-1b-pab-1992023141015.jpg "Immunocytochemistry/Immunofluorescence: Histone H2B type 1B Antibody [NBP3-23376] - Immunocytochemical labeling of Histone H2B type 1B in methanol and acetone fixed rat A7r5 cells. The cells were labeled with rabbit polyclonal Histone H2B type 1B (C-terminus) antibody (NBP3-23376), then the antibody was detected using appropriate secondary antibody conjugated to DyLight(R) 594.")
![Western Blot: Histone H2B type 1B Antibody [NBP3-23376] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23376_rabbit-histone-h2b-type-1b-pab-19920231759447.jpg)
![Western Blot: Histone H2B type 1B [p Ser14] Antibody (RM238) [NBP2-61501] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61501 at 0.5 ug/ml](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-p-Ser14-Antibody-RM238-Western-Blot-NBP2-61501-img0004.jpg "Western Blot: Histone H2B type 1B [p Ser14] Antibody (RM238) [NBP2-61501] - Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole using NBP2-61501 at 0.5 ug/ml")
![Immunocytochemistry/ Immunofluorescence: Histone H2B type 1B [p Ser14] Antibody (RM238) - Azide and BSA Free [NBP2-61501] - Immunocytochemistry of HeLa cells using Clone RM238 (red) and DAPI (blue). Actin filaments have been labeled with fluorescein phalloidin (green).](http://images.novusbio.com/fullsize/nbp2-61501_rabbit-monoclonal-histone-h2b-type-1b-p-ser14-antibody-rm238-immunocytochemistry-immunofluorescence-18920252338179.png)
![Western Blot: Histone H2B type 1B Antibody [NBP3-23377] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23377_rabbit-histone-h2b-type-1b-pab-119202316331618.jpg "Western Blot: Histone H2B type 1B Antibody [NBP3-23377] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).")
![Western Blot: Histone H2B type 1B [p Ser36] Antibody [NBP3-23378] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).](http://images.novusbio.com/fullsize/nbp3-23378_rabbit-histone-h2b-type-1b-p-ser36-pab-18920231735528.jpg "Western Blot: Histone H2B type 1B [p Ser36] Antibody [NBP3-23378] - Western blot analysis of human Jurkat cells treated with calyculin A (100 nM) for 30 min. (lanes 1, 3, & 5) then the blots were treated with lambda phosphatase (lanes 2, 4, & 6). The blots were probed with anti-Histone H2B type 1B (C-terminus) (lanes 1 & 2), anti-Histone H2B type 1B (Ser-36) (lanes 3 & 4), and anti-Histone H2B type 1B (a.a. 33-47) (lanes 5 & 6).")
![Western Blot: Histone H2B type 1B Antibody [NBP2-98972] - Anti-Histone H2B type 1B rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 15 kDa](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-Antibody-Western-Blot-NBP2-98972-img0003.jpg "Western Blot: Histone H2B type 1B Antibody [NBP2-98972] - Anti-Histone H2B type 1B rabbit polyclonal antibody at 1:500 dilution. Lane A: Jurkat Whole Cell Lysate Lane B: 293 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 14 kDa. Observed band size: 15 kDa")
![Immunoprecipitation: Histone H2B type 1B Antibody [NBP2-98972] - Histone H2B type 1B was immunoprecipitated using: Lane A: 0.5 mg MCF-7 Whole Cell Lysate. 1 ul anti-Histone H2B type 1B rabbit polyclonal antibody and 60 ug of Immunomagnetic beads. Protein A/G. Primary antibody: Anti-Histone H2B type 1B rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 21 kDa. Observed band size: 25 kDa](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-Antibody-Immunoprecipitation-NBP2-98972-img0004.jpg)
![Western Blot: Histone H2B type 1B [Monomethyl Glu105] Antibody [NBP3-15983] - Western blot analysis of extracts of various cell lines, using Histone H2B type 1B antibody (NBP3-15983) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-[Monomethyl-Glu105]-Antibody-Western-Blot-NBP3-15983-img0001.jpg "Western Blot: Histone H2B type 1B [Monomethyl Glu105] Antibody [NBP3-15983] - Western blot analysis of extracts of various cell lines, using Histone H2B type 1B antibody (NBP3-15983) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: Histone H2B type 1B Overexpression Lysate (Adult Normal) [NBP2-06573] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H2B type 1B.](http://images.novusbio.com/fullsize/Histone-H2B-type-1B-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-06573-img0001.jpg "Western Blot: Histone H2B type 1B Overexpression Lysate (Adult Normal) [NBP2-06573] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Histone H2B type 1B.")