Histone H2B type 1B [ac Lys20] Antibody (RM235)

Western Blot: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Western Blot of acid extracts from HeLa cells treated (+) or untreated (-) with sodium butyrate, using NBP2-61496 at 0.5 ug/ml

Immunocytochemistry/Immunofluorescence: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - Immunocytochemical staining of HeLa cells treated with sodium butyrate, using NBP2-61496 (red). Actin filaments have been labeled with fluorescein phalloidin (green).

Multiplex Immunoassay: Histone H2B type 1B [ac Lys20] Antibody (RM235) [NBP2-61496] - NBP2-61496 specifically reacts to Histone H2B type 1B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.

• Reactivity: Hu, Vb
• Applications: WB, ELISA, ICC/IF, Func
• Clone: RM235
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Histone H2B type 1B [ac Lys20] Antibody (RM235) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: An acetyl-peptide corresponding to Histone H2B type 1B [ac Lys20]
• Modification: ac Lys20
• Specificity: This antibody reacts to Histone H2B type 1B acetylated at Lysine 20. No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: H2BC3
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.2 ug/mL-1 ug/mL
  • Immunocytochemistry/ Immunofluorescence 1 ug/ml - 2 ug/ml
  • Multiplex Immunoassay 0.1 ug/ml - 0.5 ug/ml
  • Western Blot 0.5 ug/ml - 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS, 1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Histone H2B type 1B [ac Lys20] Antibody (RM235)

• H2B histone family, member F
• H2B/fHistone H2B.f
• H2BFFH2B.1
• histone 1, H2bb
• histone cluster 1, H2bb
• histone H2B type 1-B
• Histone H2B.1
• MGC119804

Background

The HIST1H2BB gene encodes a 126 amino acid long, 13 kDA histone H2B type 1-B that is critical in the nucleosome by functioning in transcription regulation, DNA replication and repair, as well as chromosomal stability. It participates in histone modification, apoptosis and authophagy, protein stability, RSF complex binding to the centromer, and signal transduction Activin A signaling regulation. HIST1H2BB is known to interact with genes EP300, ERCC6, DYRK1A, NFKB2, and TNFRSF1A. It has been researched regarding its role to lupus, immunodeficiency, cockayne syndrome, and hemochromatosis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: H2BC3