Hu,
Mu,
Rt,
PoSpecies Glossary
Reactivity: Hu, Mu, Rt, Po
WB, Simple Western, ICC/IF, IHC, IP, KO, Mycoplasma
Applications: WB, Simple Western, ICC/IF, IHC, IP, KO, Mycoplasma
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - miR-513c-5p negatively regulates caspase-1 expression in HUVECs. (A) The expression of miR-513c-5p after NC, miR-513c-5p mimics, INC & miR-513c-5p inhibitor transfection was evaluated by qRT-PCR. (B) The expression level of caspase-1 mRNA after NC, miR-513c-5p mimics, INC & miR-513c-5p inhibitor transfection was detected by qRT-PCR. (C,D) Expression of caspase-1 in HUVECs were detected after transfection NC, miR-513c-5p mimics, INC, miR-513c-5p inhibitor by immunofluorescence staining (magnification, ×40). Scale bar = 200 μm. (E,F) Protein expression levels of caspase-1 & GSDMD were examined by Western blot. (G) The expression of IL-1 beta & IL-18 was detected by ELISA. *p 0.05; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-31020241613629.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Pyroptosis is occurred in mouse corneas of C. albicans keratitis. (A) RT-qPCR analysis of the mRNA levels of pyroptosis-associated genes (ASC/CASP1/GSDMD/IL-1 beta /IL-18) in mouse corneas at 0 (control), 1, 3, & 7 dpi (n = 3). (B,C) Western blot detecting pyroptosis-related proteins of ASC, cleaved CASP1, cleaved IL-1 beta , cleaved IL-18, F-GSDMD & N-GSDMD in mouse corneas at 0 (control), 1, 3, & 7 dpi (n = 3). (D) Double-immunofluorescence staining of CASP1 & TUNEL in C. albicans infected-corneas compared with mock-infected controls (n = 3; Scale bar = 20 μm; magnification 400×). CASP1: caspase-1; Clv-CASP1:cleaved CASP1; Clv-IL-1 beta :cleaved IL-1 beta ; Clv-IL-18:cleaved IL-18; F-GSDMD: p53 form of GSDMD; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416114767.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - NLRP3 knockdown attenuates the pyroptosis in mouse C. albicans keratitis. (A–C) RT-qPCR analysis & western blot showing the mRNA & protein levels of pyroptosis-related molecules in C. albicans-infected corneas pretreated with Ad-GFP-shRNA & Ad-NLRP3- shRNA compared with mock controls (n = 3). (D) Immunofluorescence staining of ASC, CASP1 & GSDMD in C. albicans-infected corneas pretreated with Ad-GFP-shRNA & Ad-NLRP3- shRNA compared with mock controls (n = 3). (E) Double-immunofluorescence staining of CASP1 & TUNEL in the mice cornea of Ad-NLRP3-shRNA group compared with the Ad-GFP-shRNA group (n = 3). Scale bar = 20 μm; magnification 400×. FK: fungal keratitis. CASP1: caspase-1; Clv-CASP1:cleaved CASP1; Clv-IL-1 beta :cleaved IL-1 beta ; Clv-IL-18:cleaved IL-18; F-GSDMD: p53 form of GSDMD; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-31020241613636.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Knockdown of miR-513c-5p promotes pyroptosis of VECs & DVT formation by increasing caspase-1. (A) The expression level of miR-513c-5p in vascular tissue was detected by qRT-PCR in DVT mice treated with INC, miR-513c-5p inhibitor, respectively. (B) Expression of miR-513c-5p in PBMCs was detected by qRT-PCR in DVT mice treated with INC, miR-513c-5p inhibitor, respectively. (C) Confocal microscopy images of miR-513c-5p expression in vascular tissues (miR-513c-5p, red; DAPI, blue) (magnification, ×200). Scale bars = 100 μm. (D) H&E staining of serial cross sections of inferior vena cava (IVC) from DVT mice treated with INC, miR-513c-5p inhibitor at 48 h (magnification, ×40). Scale bars = 500 μm. (E) Representative images of thrombi in each treatment group were detected by vascular ultrasound at 48 h post-operation. (F) Thrombus length & weight at 48 h post-operation in the different treatment groups (n = 15 in each group). (G) Caspase-1 mRNA level was determined by qRT-PCR in vascular tissue of each treatment group. (H) Caspase-1 & GSDMD protein levels were determined by Western blot in vascular tissue of DVT mice treated with INC, miR-513c-5p inhibitor. (I) IL-1 beta & IL-18 protein levels in vascular tissue were determined by ELISA in INC, miR-513c-5p inhibitor treated DVT mice models, respectively. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35445025), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416114772.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Pyroptosis & IL-1 beta release is dependent on the B. pseudomallei T3SS-3.hMDMs were infected with B. pseudomallei (MOI 300) for 3h. (A & B) Cell death induction & intracellular bacterial burden were determined at 0 & 3h p.i. Shown are the median & interquartile range of at least three independent experiments with different donors performed in technical duplicates. (C) IL-1 beta secretion was determined 3h p.i. Data are presented as median & interquartile range of four independent experiments with different donors performed in duplicates. (D) Caspase-1 & gasdermin-D processing were investigated 3h p.i. Lysates were re-probed for beta -actin. For immunoblot analysis one representative experiment of at least three with different donors is shown. (E) Growth analysis of wt & delta bsaL was performed. Shown are mean values of three independent experiments performed in technical triplicates. (*p < 0.05, **p < 0.01). n.i. (not infected), wt (wild type), b.d. (below detection), hours (h), p.i. (post infection), GSDMD (gasdermin D). Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33137811), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416121947.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Priming is not required for NLRP3 inflammasome activation in human monocytes in vitro. (A, B) Undifferentiated THP-1 cells (n=6 independent biological replicates) & (C, D) primary CD14+ monocytes (n=6 independent biological replicates (each point represents a different blood donor)) were left untreated or primed with LPS (1 µg/ml, 4 h) prior to treatment with nigericin (10 µM, 45 min) to activate the NLRP3 inflammasome. (A, C) IL-1 beta & IL-18 were measured by ELISA & cell death was measured by LDH assay & shown as percentage relative to total cell death, mean ± S.D., *P < 0.05; **P < 0.01; ***P < 0.001; ns (non significant) using one-way ANOVA comparing all groups. (B, D) Western blot analysis for mIL-18 (18 kDa), pro-IL-18 (24 kDa), mIL-1 beta (17 kDa), pro-IL-1 beta (34 kDa), mCaspase-1 (20 kDa), pro-Caspase-1 (45 kDa), GSDMD full length (FL, 53 kDa), GSDMD N-terminus (NT, 31 kDa), UBE2L3 (17.9 kDa), NLRP3 (113 kDa), as well as loading control beta -actin (42 kDa). Blots are representative of at least 3 independent biological experiments & in case of monocytes 3 different blood donors. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/33101286), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-310202416121939.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-33422] - Heat-killed C. albicans (HKCA) activates NLRP3 inflammasome & induces pyroptosis in human corneal epithelial cells (HCECs). (A) The mRNA expression of NLRP3 in HCECs challenged with HKCA at an MOI of 1:500, 1:50, 1:5, 2:1, or 20:1 respectively for 4 hours was evaluated by RT-qPCR (n = 5). (B–D) The mRNA & protein expression of NLRP3 in HCECs exposed to HKCA (MOI = 20) for 0 (control), 2, 4, 8, 12, or 24 h (n = 3). (E) NLRP3 fluorescence intensity was evaluated using immunofluorescent staining for different times (12–36 h). (n = 3; Scale bar = 20 μm; magnification 400×). (F) Lactate dehydrogenase (LDH) of HCECs treated with HKCA (MOI = 20) for 24 h (n = 6). (G) The mRNA levels of ASC, CASP1, IL-1 beta , IL-18 & GSDMD in HCECs exposed to HKCA (MOI = 20) for different times (n = 3). (H,I) The protein expression of pyroptosis-related proteins (ASC, cleaved CASP1, N-GSDMD, cleaved IL-1 beta & cleaved IL-18) was examined by western blot (n = 3). CASP1: caspase-1; Clv-CASP1: cleaved CASP1; Clv-IL-1 beta : cleaved IL-1 beta ; Clv-IL-18: cleaved IL-18; N-GSDMD: cleaved p30 form of GSDMD. All values are presented as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001 vs. control group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/35463001), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-33422_rabbit-polyclonal-gsdmdc1-antibody-31020241612228.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-80427] - Analysis of Gasdermin D (N terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysateLane 2: human kidney tissue lysate](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Western-Blot-NBP2-80427-img0009.jpg "Western Blot: GSDMDC1 Antibody [NBP2-80427] - Analysis of Gasdermin D (N terminal) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: SiHa cell lysateLane 2: human kidney tissue lysate")
![Immunocytochemistry/Immunofluorescence: GSDMDC1 Antibody [NBP2-80427] - Staining of Gasdermin D in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-37, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-80427-img0002.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in Mouse colon tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0007.jpg)
![Flow Cytometry: GSDMDC1 Antibody [NBP2-80427] - Analysis of GSDMDC1 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes. Unlabeled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Flow-Cytometry-NBP2-80427-img0001.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in rat stomach tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0003.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human tonsil tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed wit](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0004.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human prostate carcinoma tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0005.jpg)
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody [NBP2-80427] - Analysis in human esophagus tissue using GSDMDC1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Immunohistochemistry-Paraffin-NBP2-80427-img0006.jpg)
![Western Blot: GSDMDC1 Antibody [NBP2-93428] - Western blot analysis of extracts of various cell lines, using GSDMDC1 antibody (NBP2-93428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/GSDMDC1 Antibody-Western Blot-NBP2-93428-img0004.jpg "Western Blot: GSDMDC1 Antibody [NBP2-93428] - Western blot analysis of extracts of various cell lines, using GSDMDC1 antibody (NBP2-93428) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of PC-12 cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-4220251430428.jpg)
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of HeLa cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-4220251430468.jpg)
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of PC-12 cells using GSDMD Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-4220251429026.jpg)
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of A-431 cells using GSDMD (Full Length+N terminal) Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-422025143044.jpg)
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of U2OS cells using GSDMD Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-42202514251883.jpg)
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of PC-3 cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-42202514275221.jpg)
![Western Blot: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Western blot analysis of recombinant GSDMD-FL and N+C Protein using GSDMD Rabbit pAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-4220251429041.jpg)
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody - Azide and BSA Free [NBP2-93428] - Immunofluorescence analysis of NIH/3T3 cells using GSDMD Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-93428_rabbit-polyclonal-gsdmdc1-antibody-422025143185.jpg)
![Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody (3G1A6) - Cleaved, N-terminal [NBP3-33474] - Confocal imaging of THP-1 cells (treated with PMA and LPS) and THP-1 cells (untreated) cells using [KO Validated] GSDMDC1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-33474_rabbit-gsdmdc1-mab-3g1a6-cleaved-n-terminal-31120251744488.jpg "Immunocytochemistry/ Immunofluorescence: GSDMDC1 Antibody (3G1A6) - Cleaved, N-terminal [NBP3-33474] - Confocal imaging of THP-1 cells (treated with PMA and LPS) and THP-1 cells (untreated) cells using [KO Validated] GSDMDC1 Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.")
![Western Blot: GSDMDC1 Antibody (3G1A6) - Cleaved, N-terminal [NBP3-33474] - Western Blot analysis of lysates from THP-1 cells using [KO Validated] GSDMDC1 Rabbit mAb at 1:72000 dilution incubated overnight at 4C. THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight and LPS (1 ug/ml) at 37C for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-33474_rabbit-gsdmdc1-mab-3g1a6-cleaved-n-terminal-3112025173182.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GSDMDC1 Antibody (3G1A6) - Cleaved, N-terminal [NBP3-33474] - Western Blot analysis of lysates from wild type (WT) and Cleaved Gasdermin D knockout (KO) THP-1 cells using [KO Validated] GSDMDC1 Rabbit mAb at 1:72000 dilution incubated at room temperature for 1.5 hours. THP-1 cells and Gasdermin D knockout (KO) THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight and LPS (1 ug/ml) at 37C for 6 hours.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 30 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.](http://images.novusbio.com/fullsize/nbp3-33474_rabbit-gsdmdc1-mab-3g1a6-cleaved-n-terminal-31120251704759.jpg)
![Western Blot: GSDMDC1 Antibody (HL1430) - Azide and BSA Free [NBP3-25478] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [HL1430] (NBP3-25478) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25478_rabbit-gsdmdc1-mab-hl1430-azide-and-bsa-free-2120249351326.jpg "Western Blot: GSDMDC1 Antibody (HL1430) - Azide and BSA Free [NBP3-25478] - Untreated (-) and treated (+) THP-1 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [HL1430] (NBP3-25478) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunohistochemistry-Paraffin: GSDMDC1 Antibody (HL1430) - Azide and BSA Free [NBP3-25478] - Gasdermin D antibody [HL1430] detects Gasdermin D protein at cell membrane and secreted by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. Gasdermin D stained by Gasdermin D antibody [HL1430] (NBP3-25478) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25478_rabbit-gsdmdc1-mab-hl1430-azide-and-bsa-free-2120249393571.jpg)
![Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of A-431 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-8A8A5-Western-Blot-NBP3-16070-img0005.jpg "Western Blot: GSDMDC1 Antibody (8A8A5) [NBP3-16070] - Western blot analysis of extracts of A-431 cells, using GSDMDC1 antibody (NBP3-16070) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![<b>Biological Strategies Validation. </b>Western Blot: Rabbit GSDMDC1 mAb (5U7R7) [NBP3-16070] - Analysis of extracts of THP-1, using GSDMD antibody at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 60s.](http://images.novusbio.com/fullsize/nbp3-16070_rabbit-gsdmdc1-mab-8a8a5-western-blot-30320237391.jpg)
![Western Blot: GSDMDC1 Antibody (5U7R7) [NBP3-16070] - Western blot analysis of lysates from PC-3 cells using GSDMDC1 Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 45s.](http://images.novusbio.com/fullsize/nbp3-16070_rabbit-gsdmdc1-mab-5u7r7-52202511361238.jpg)
![SDS-Page: Recombinant Human GSDMDC1 Protein [H00079792-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-GSDMDC1-Protein-SDS-Page-H00079792-P01-img0002.jpg "SDS-Page: Recombinant Human GSDMDC1 Protein [H00079792-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: GSDMDC1 Antibody [H00079792-D01P] - Analysis of GSDMD expression in transfected 293T cell line by GSDMD polyclonal antibody.Lane 1: GSDMDC1 transfected lysate(52.80 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Western-Blot-H00079792-D01P-img0002.jpg "Western Blot: GSDMDC1 Antibody [H00079792-D01P] - Analysis of GSDMD expression in transfected 293T cell line by GSDMD polyclonal antibody.Lane 1: GSDMDC1 transfected lysate(52.80 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: GSDMDC1 Antibody [NBP2-14973] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GSDMDC1-Antibody-Western-Blot-NBP2-14973-img0002.jpg "Western Blot: GSDMDC1 Antibody [NBP2-14973] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Orthogonal Strategies Validation. </b>Western Blot: GSDMDC1 Antibody [NBP2-14973] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Gasdermin D antibody [N1N3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp2-14973_rabbit-polyclonal-gsdmdc1-antibody-1610202419405758.jpg)
![Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS](http://images.novusbio.com/fullsize/nbp3-33450_rabbit-gsdmdc1-mab-8k8c6-522025125471.jpg "Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of lysates from THP-1 cells using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. THP-1 cells were treated by LPS and Nigericin.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS")
![Western Blot: GSDMDC1 Antibody (8K8C6) [NBP3-33450] - Western blot analysis of recombinant GSDMD Protein using GSDMD (Full Length+C terminal) Rabbit mAb at 1:2000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 10 ng per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.WB samples for antibody validation are kindly provided by Dr. Feng Shao, NIBS](http://images.novusbio.com/fullsize/nbp3-33450_rabbit-gsdmdc1-mab-8k8c6-5220251141144.jpg)
![Western Blot: GSDMDC1 Antibody (PD00-18) - N-terminal [NBP3-32369] - Western blot analysis of GSDMDC1 on different lysates with Rabbit anti-GSDMDC1 antibody (NBP3-32369) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32369) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32369_rabbit-gsdmdc1-mab-pd00-18-n-terminal-278202416331282.jpg "Western Blot: GSDMDC1 Antibody (PD00-18) - N-terminal [NBP3-32369] - Western blot analysis of GSDMDC1 on different lysates with Rabbit anti-GSDMDC1 antibody (NBP3-32369) at 1/1,000 dilution. Lane 1: SiHa cell lysate Lane 2: Jurkat cell lysate Lane 3: THP-1 cell lysate Lane 4: PC-3M cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: PC-12 cell lysate Lane 7: L6 cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 53/30 kDa Observed band size: 53/30 kDa Exposure time: 43 seconds; ECL; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32369) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunoprecipitation:GSDMDC1 AntibodyNBP3-30246] -Detection of human GSDMD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GSDMD antibody (NBP3-30246 lot 1) used for IP at 2 µg per reaction. GSDMD was also immunoprecipitated by rabbit anti-GSDMD.. For blotting immunoprecipitated GSDMD, NBP3-30246 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30246_rabbit-gsdmdc1-pab-301220241229396.jpg "Immunoprecipitation:GSDMDC1 AntibodyNBP3-30246] -Detection of human GSDMD by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-GSDMD antibody (NBP3-30246 lot 1) used for IP at 2 µg per reaction. GSDMD was also immunoprecipitated by rabbit anti-GSDMD.. For blotting immunoprecipitated GSDMD, NBP3-30246 was used at 0.1 mg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")