| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4339-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-03731-20ul | |||
HAF007). A specific band was detected for GRK2 at approximately 79 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat GRK2 Monoclonal Antibody (Catalog # MAB4339) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for GRK2 at approximately 79 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4339-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-57283-25ul | |||
![Western Blot: GRK2 Antibody (3F8) [NBP2-37611] - Analysis using GRK2 mouse mAb against Hela (1), Jurkat (2), MOLT4 (3), RAJI (4), THP-1 (5), L1210 (6), Cos7 (7), PC-12 (8), and NIH/3T3 (9) cell lysate.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Western-Blot-NBP2-37611-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of NIH/3T3 cells using GRK2 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Immunocytochemistry-Immunofluorescence-NBP2-37611-img0003.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody (3F8) [NBP2-37611] - Analysis of endometrial cancer tissues (left) and cervical cancer tissues (right) using GRK2 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-Immunohistochemistry-Paraffin-NBP2-37611-img0005.jpg)
![ELISA: GRK2 Antibody (3F8) [NBP2-37611] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/GRK2-Antibody-3F8-ELISA-NBP2-37611-img0001.jpg)
![Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2), beta -arrestin2, & 8-hydroxy-2′deoxyguanosine (8-OHdG) in two patients with takotsubo syndrome who underwent endomyocardial biopsy in both the acute & recovery phases. Micrographs showing immunofluorescence stainings of GRK2 & beta -arrestin2, & immunohistochemical staining of 8-OHdG in the acute & recovery phases. Arrowheads (yellow) indicate positive signals for GRK2 & beta -arrestin2 on the cell membrane, & Arrowheads (red) indicate 8-OHdG-positive nuclei. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37611_mouse-monoclonal-grk2-antibody-3f8-310202415371959.jpg)
![Immunocytochemistry/ Immunofluorescence: GRK2 Antibody (3F8) - BSA Free [NBP2-37611] - Localization of G protein-coupled receptor kinase 2 (GRK2). (a) Immunohistofluorescence staining for GRK2 using the specific antibody. GRK2 (red) in the takotsubo syndrome (TTS) & dilated cardiomyopathy (DCM) groups were observed not only in the cytoplasm but also on the cell membrane, which was confirmed by colocalization with wheat germ agglutinin (WGA) (green) (Arrowheads). Blue staining localized to 4′,6-diamidino-2-phenylindole (DAPI). (b) Quantification for GRK2 positive stained area in the myocardium. The box represents the 25th & 75th percentiles & the line the median value. Whiskers correspond to the 25th percentile minus 1.5 times interquartile range (IQR) & to the 75th percentile plus 1.5 IQR. (c) Quantification for membranous GRK2 positive cardiomyocyte. *P < 0.001 vs. the normal control (NC) group. †P < 0.001 vs. the DCM group. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/30143703), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp2-37611_mouse-monoclonal-grk2-antibody-3f8-310202416121924.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425483.jpg "Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded mouse lymph node. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-212024939175.jpg)
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249432853.jpg)
![Western Blot: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - Various tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425466.jpg)
![Immunocytochemistry/Immunofluorescence: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 10 min. Green: GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI . Scale bar= 10um.](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-2120249425437.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody (HL1562) - Azide and BSA Free [NBP3-25491] - GRK2 antibody [HL1562] detects GRK2 protein by immunohistochemical analysis. Sample: Paraffin-embedded rat tissues. GRK2 stained by GRK2 antibody [HL1562] (NBP3-25491) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25491_rabbit-grk2-mab-hl1562-azide-and-bsa-free-212024940814.jpg)
![Western Blot: GRK2 Antibody [NBP3-03731] - Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (NBP3-03731) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](http://images.novusbio.com/fullsize/GRK2-Antibody-Western-Blot-NBP3-03731-img0006.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GRK2 Antibody [NBP3-03731] - Western blot analysis of extracts from normal (control) and GRK2 knockout (KO) 293T cells, using GRK2 antibody (NBP3-03731) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded mouse kidney using GRK2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/GRK2-Antibody-Immunohistochemistry-Paraffin-NBP3-03731-img0003.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded rat brain using GRK2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/GRK2-Antibody-Immunohistochemistry-Paraffin-NBP3-03731-img0001.jpg)
![Immunohistochemistry-Paraffin: GRK2 Antibody [NBP3-03731] - Paraffin-embedded human esophageal cancer using GRK2 antibody at dilution of 1:200 (40x lens).](http://images.novusbio.com/fullsize/GRK2-Antibody-Immunohistochemistry-Paraffin-NBP3-03731-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: GRK2 Antibody [NBP3-03731] - Analysis of extracts of various cell lines, using GRK2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.](http://images.novusbio.com/fullsize/GRK2-Antibody-Knockout-Validated-NBP3-03731-img0005.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0005.jpg "<b>Biological Strategies Validation. </b>Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - GRK2 (phospho Ser670) antibody [HL1035] detects GRK2 (phospho Ser670) protein at cell membrane and cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse thymus gland. GRK2 (phospho Ser670) stained by GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:200. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Immunohistochemistry-Paraffin-NBP3-13688-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0001.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0003.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 (phospho Ser670) antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/GRK2-p-Ser670-Antibody-HL1035-Western-Blot-NBP3-13688-img0004.jpg)
![Western Blot: GRK2 [p Ser670] Antibody (HL1035) [NBP3-13688] - Various tissue extracts (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [HL1035] (NBP3-13688) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-13688_rabbit-grk2-p-ser-670-mab-hl1035-9102024201574.jpg)
![Lane 1: Marker [kDa] 250,130,95,72,55,36,28,17,10Lane 2: RT-4Lane 3: U-251MGLane 4: Human PlasmaLane 5: LiverLane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-24887_rabbit-grk2-pab-20122023150269.jpg)
![SDS-Page: Recombinant Human GRK2 Protein [H00000156-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-GRK2-Protein-SDS-Page-H00000156-P01-img0001.jpg "SDS-Page: Recombinant Human GRK2 Protein [H00000156-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.](http://images.novusbio.com/fullsize/GRK2-Antibody-Western-Blot-NB100-2831-img0001.jpg "Western Blot: GRK2 Antibody [NB100-2831] - Staining of Daudi cell lysate (35 ug protein in RIPA buffer). Antibody at 2 ug/mL. Detected by chemiluminescence. Approx 80 kDa band (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizing peptide.")
![Immunocytochemistry/Immunofluorescence: GRK2 Antibody [NBP2-57283] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GRK2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57283-img0001.jpg "Immunocytochemistry/Immunofluorescence: GRK2 Antibody [NBP2-57283] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.")
![ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] - Samples were spiked with high concentrations of Rat GRK2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-66688_rat-grk2-elisa-kit-colorimetric-13220241618489.jpg "ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] - Samples were spiked with high concentrations of Rat GRK2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat GRK2 ELISA Kit (Colorimetric) [NBP2-66688] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-66688_rat-grk2-elisa-kit-colorimetric-4320248574392.jpg)
