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![Western Blot: Glypican 1 Antibody [NBP1-33197] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0012.jpg "<b>Genetic Strategies Validation. </b>Western Blot: Glypican 1 Antibody [NBP1-33197] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the publication: PMID: 22351761, 26203194 and 21932778")
![<b>Biological Strategies Validation and Genetic Strategies Validation.</b>Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3].The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody [NBP1-33197] - Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunofluorescent analysis.Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.Green: Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar= 10 um.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-33197-img0028.jpg)
![Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Paraffin-embedded human normal pancreas (left) and pancreatic cancer (right).Glypican 1 stained by Glypican 1 antibody [N3C3] diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunohistochemistry-Paraffin-NBP1-33197-img0029.jpg)
![Flow Cytometry: Glypican 1 Antibody [NBP1-33197] - Sample: A431 cell. Black: Unlabelled sample was used as a control. Red: Glypican 1 antibody [N3C3]dilution: 1:100. Acquisition of 20,000 events were collected using a Dylight 488-conjugated secondary antibody for FACS analysis.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Flow-Cytometry-NBP1-33197-img0017.jpg)
![Western Blot: Glypican 1 Antibody [NBP1-33197] - Mouse tissue extracts (50 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0015.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3]. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0023.jpg)
![Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell extract and conditioned medium (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody ] was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0025.jpg)
![Western Blot: Glypican 1 Antibody [NBP1-33197] - U87-MG whole cell and membrane extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0026.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Glypican 1 Antibody [NBP1-33197] - Untreated (-) and treated (+) MDA-MB-231 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3]. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.The observed M.W. is based on the following publications: PMID: 22351761, 26203194 and 21932778](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-33197-img0027.jpg)
![ELISA: Glypican 1 Antibody [NBP1-33197] - An ELISA plate is coated with MCF-7 and A431 cells. The coated cells are detected with Glypican 1 antibody at concentration ranged from 7.5 to 240 ng/mL.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-ELISA-NBP1-33197-img0016.jpg)
![Immunoprecipitation: Glypican 1 Antibody [NBP1-33197] - Immunoprecipitation of Glypican 1 protein from MCF-7 whole cell extracts using 5 ug of Glypican 1 antibody [N3C3].](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunoprecipitation-NBP1-33197-img0021.jpg)
![Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP1-33197] - Glypican 1 antibody [N3C3] detects Glypican 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human pancreatic cancer.Glypican 1 stained by Glypican 1 antibody [N3C3] (NBP1-33197) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp1-33197_rabbit-polyclonal-glypican-1-antibody-91020242091432.jpg)
![Western Blot: Glypican 1 Antibody [NBP1-33197] - A431 whole cell extracts (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody [N3C3] (NBP1-33197) diluted at 1:1500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp1-33197_rabbit-polyclonal-glypican-1-antibody-910202420122095.jpg)
![Western Blot: Glypican 1 Antibody [NBP3-13127] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP3-13127-img0004.jpg "<b>Independent Antibodies Validation. </b>Western Blot: Glypican 1 Antibody [NBP3-13127] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP3-13127] - Human pancreas, normal (left) and cancer (right). Glypican 1 antibody (NBP3-13127) diluted at 1:250. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunohistochemistry-Paraffin-NBP3-13127-img0001.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: Glypican 1 Antibody [NBP3-13127] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP3-13127-img0002.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: Glypican 1 Antibody [NBP3-13127] - Non-transfected (-) and transfected (+) MCF-7 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Glypican 1 antibody (NBP3-13127) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP3-13127-img0003.jpg)
![Western Blot: Glypican 1 Antibody [NBP1-42718] - Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4 C. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Western-Blot-NBP1-42718-img0004.jpg "Western Blot: Glypican 1 Antibody [NBP1-42718] - Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4 C. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.")
![Immunohistochemistry: Glypican 1 Antibody [NBP1-42718] - Tissue: skin. Fixation: formalin fixed paraffin embedded. Primary antibody: anti-Glypican antibody at 10 ug/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Staining: Glypican as precipitated red signal with hematoxylin purple nuclear counterstain.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunohistochemistry-NBP1-42718-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody [NBP3-17035] - Analysis in human skin and lymph node tissues using Anti-GPC1 antibody. Corresponding GPC1 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunocytochemistry-Immunofluorescence-NBP3-17035-img0001.jpg "Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody [NBP3-17035] - Analysis in human skin and lymph node tissues using Anti-GPC1 antibody. Corresponding GPC1 RNA-seq data are presented for the same tissues.")
![Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP3-17035] - Staining of human lymph node shows low expression as expected.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunohistochemistry-Paraffin-NBP3-17035-img0002.jpg)
![Immunohistochemistry-Paraffin: Glypican 1 Antibody [NBP3-17035] - Staining of human skin shows high expression.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-Immunohistochemistry-Paraffin-NBP3-17035-img0003.jpg)
![Western Blot: Glypican 1 Antibody (S03-2E8) [NBP3-19539] - Western blot detection of Glypican 1 in Jurkat, Hela cell lysates using NBP3-19539 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 55kDa.](http://images.novusbio.com/fullsize/Glypican-1-Antibody-S03-2E8-Western-Blot-NBP3-19539-img0002.jpg "Western Blot: Glypican 1 Antibody (S03-2E8) [NBP3-19539] - Western blot detection of Glypican 1 in Jurkat, Hela cell lysates using NBP3-19539 (1:1000 diluted). Predicted band size: 62kDa. Observed band size: 55kDa.")
![Immunocytochemistry/Immunofluorescence: Glypican 1 Antibody (S03-2E8) [NBP3-19539] - Immunofluorescence of Glypican 1 (green) in Hela using NBP3-19539 at dilution 1/20, and DAPI (blue)](http://images.novusbio.com/fullsize/nbp3-19539_rabbit-glypican-1-mab-s03-2e8-113202515145455.jpg)
![ELISA: Human Glypican 1 ELISA Kit (Colorimetric) [NBP2-82183] - Samples were spiked with high concentrations of Human Glypican 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp2-82183_human-glypican-1-elisa-kit-colorimetric-132202416254028.jpg "ELISA: Human Glypican 1 ELISA Kit (Colorimetric) [NBP2-82183] - Samples were spiked with high concentrations of Human Glypican 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Human Glypican 1 ELISA Kit (Colorimetric) [NBP2-82183] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp2-82183_human-glypican-1-elisa-kit-colorimetric-4320249192826.jpg)