NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="Endothelin-3 was detected in immersion fixed LNCaP human prostate cancer cell line using Mouse Anti-Human Endothelin-3 Monoclonal Antibody (Catalog # MAB5579) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell secretion. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5579-SP | |||
![Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - Analysis of EDN3 expression in transfected 293T cell line by EDN3 monoclonal antibody (M01), clone 2A6-2A4.Lane 1: EDN3 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Endothelin-3-Antibody-2A6-2A4-Western-Blot-H00001908-M01-img0006.jpg "Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - Analysis of EDN3 expression in transfected 293T cell line by EDN3 monoclonal antibody (M01), clone 2A6-2A4.Lane 1: EDN3 transfected lysate(25.5 KDa).Lane 2: Non-transfected lysate.")
![Western Blot: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - EDN3 monoclonal antibody (M01), clone 2A6-2A4 Analysis of EDN3 expression in LNCaP.](http://images.novusbio.com/fullsize/Endothelin-3-Antibody-2A6-2A4-Western-Blot-H00001908-M01-img0005.jpg)
![Immunoprecipitation: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - Analysis of EDN3 transfected lysate using anti-EDN3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN3 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Endothelin-3-Antibody-2A6-2A4-Immunoprecipitation-H00001908-M01-img0003.jpg)
![ELISA: Endothelin-3 Antibody (2A6-2A4) [H00001908-M01] - Detection limit for recombinant GST tagged EDN3 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Endothelin-3-Antibody-2A6-2A4-ELISA-H00001908-M01-img0004.jpg)
![Western Blot: Endothelin-3 Antibody [H00001908-D01P] - Analysis of EDN3 expression in human placenta.](http://images.novusbio.com/fullsize/Endothelin-3-Antibody-Western-Blot-H00001908-D01P-img0003.jpg "Western Blot: Endothelin-3 Antibody [H00001908-D01P] - Analysis of EDN3 expression in human placenta.")
![Western Blot: Endothelin-3 Antibody [H00001908-D01P] - Analysis of EDN3 expression in transfected 293T cell line by EDN3 polyclonal antibody.Lane 1: EDN3 transfected lysate(25.50 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Endothelin-3-Antibody-Western-Blot-H00001908-D01P-img0004.jpg)
![Western Blot: Endothelin-3 Antibody [NBP1-33578] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/Endothelin-3-Antibody-Western-Blot-NBP1-33578-img0003.jpg "Western Blot: Endothelin-3 Antibody [NBP1-33578] - Sample (30 ug of whole cell lysate) A: Raji 12% SDS PAGE; antibody diluted at 1:1000.")
![Western Blot: Endothelin 3 Overexpression Lysate (Adult Normal) [NBL1-10115] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Endothelin 3.](http://images.novusbio.com/fullsize/Endothelin-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10115-img0002.jpg "Western Blot: Endothelin 3 Overexpression Lysate (Adult Normal) [NBL1-10115] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Endothelin 3.")
![Western Blot: Endothelin 3 Overexpression Lysate (Adult Normal) [NBP2-04517] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Endothelin 3.](http://images.novusbio.com/fullsize/Endothelin-3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04517-img0001.jpg "Western Blot: Endothelin 3 Overexpression Lysate (Adult Normal) [NBP2-04517] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Endothelin 3.")
![SDS-Page: Recombinant Human Endothelin-3 Protein [NBP2-51961] - 15% SDS Page (3 ug)](http://images.novusbio.com/fullsize/Endothelin-3-Recombinant-Protein-SDS-Page-NBP2-51961-img0001.jpg "SDS-Page: Recombinant Human Endothelin-3 Protein [NBP2-51961] - 15% SDS Page (3 ug)")
