| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5139-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26608-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-98787-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26925 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-26928 | |||
![Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-NBP1-21374-img0039.jpg "<b>Biological Strategies Validation. </b>Western Blot: E2F-4 Antibody [NBP1-21374] - Detection of Human E2F4 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15, 50 ug) and 293T (50ug) cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: E2F-4 Antibody [NBP1-21374] - Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-E2F4 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunohistochemistry-NBP1-21374-img0037.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: E2F-4 Antibody [NBP1-21374] - Detection of human E2F4 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-E2F4 antibody NBP1-21374 used for IP at 6 ug per reaction. E2F4 was also immunoprecipitated by another rabbit anti-E2F4 antibody. For blotting immunoprecipitated E2F4, NBP1-21374 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunoprecipitation-NBP1-21374-img0040.jpg)
![<b>Genetic Strategies Validation. </b>Western Blot: E2F-4 Antibody [NBP1-21374] - Immunoblots of indicated proteins in OVCAR-8 and PEA1 cells transfected with control luciferase (Luc) and two independent E2F-4 siRNAs. Image collected and cropped by CiteAb from the following publication (//www.ncbi.nlm.nih.gov/pmc/articles/PMC6789141/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Knockdown-Validated-NBP1-21374-img0041.jpg)
![Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-278202416331292.jpg "Western Blot: E2F-4 Antibody (JE40-78) [NBP3-32285] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: A549 cell lysate Lane 3: HEK-293 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: Raji cell lysate Lane 7: HeLa cell lysate Lysates/proteins at 15 ug/Lane. Predicted band size: 44 kDa Observed band size: 60 kDa Exposure time: 3 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32285) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-E2F-4 antibody (NBP3-32285) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32285) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-298202414304159.jpg)
![Flow Cytometry: E2F-4 Antibody (JE40-78) [NBP3-32285] - Flow cytometric analysis of K-562 cells labeling E2F-4. Cells were fixed and permeabilized. Then stained with the primary antibody (NBP3-32285, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/nbp3-32285_rabbit-e2f-4-mab-je40-78-29202416372181.jpg)
![Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-262202414471533.jpg "Immunohistochemistry: E2F-4 Antibody (24E12) [NBP3-26608] - Image of E2F-4 Antibody (24E12) diluted at 1:100 and staining in paraffin-embedded human brain tissue performed. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB.")
![Flow Cytometry: E2F-4 Antibody (24E12) [NBP3-26608] - Overlay Peak curve showing MCF7 cells stained with E2F-4 Antibody (24E12) (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-282202414312787.jpg)
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody (24E12) [NBP3-26608] -Immunofluorescence staining of Hela cell with E2F-4 Antibody at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 513-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).](http://images.novusbio.com/fullsize/nbp3-26608_rabbit-e2f-4-mab-24e12-29120251248506.jpg)
![Western Blot: E2F-4 Antibody [NBP2-98787] - Lane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-NBP2-98787-img0002.jpg "Western Blot: E2F-4 Antibody [NBP2-98787] - Lane A: K562 Whole Cell LysateLane B: Raji Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:44 kDaObserved band size:53 kDa")
![Immunocytochemistry/Immunofluorescence: E2F-4 Antibody [NBP2-98787] - Immunofluorescence staining of E2F-4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human E2F-4 polyclonal antibody (dilution ratio 1:200) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-98787-img0001.jpg)
![Western Blot: E2F-4 Antibody [H00001874-D01P] - Analysis of E2F4 expression in mouse spleen.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-H00001874-D01P-img0003.jpg "Western Blot: E2F-4 Antibody [H00001874-D01P] - Analysis of E2F4 expression in mouse spleen.")
![Western Blot: E2F-4 Antibody [H00001874-D01P] - Analysis of E2F4 expression in transfected 293T cell line by E2F4 polyclonal antibody.Lane 1: E2F4 transfected lysate(44.00 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Western-Blot-H00001874-D01P-img0004.jpg)
![Immunohistochemistry: E2F-4 Antibody [NBP3-10895] - Immunohistochemical analysis of human intestine.](http://images.novusbio.com/fullsize/E2F-4-Antibody-Immunohistochemistry-NBP3-10895-img0001.jpg "Immunohistochemistry: E2F-4 Antibody [NBP3-10895] - Immunohistochemical analysis of human intestine.")
![Western Blot: E2F-4 Antibody (5B7) [H00001874-M01] - E2F4 monoclonal antibody (M01), clone 5B7 Analysis of E2F4 expression in A-549.](http://images.novusbio.com/fullsize/E2F-4-Antibody-5B7-Western-Blot-H00001874-M01-img0004.jpg "Western Blot: E2F-4 Antibody (5B7) [H00001874-M01] - E2F4 monoclonal antibody (M01), clone 5B7 Analysis of E2F4 expression in A-549.")
![Sandwich ELISA: E2F-4 Antibody (5B7) [H00001874-M01] - Detection limit for recombinant GST tagged E2F4 is approximately 0.03ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/E2F-4-Antibody-5B7-Sandwich-ELISA-H00001874-M01-img0003.jpg)
![Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850] - Western blot analysis of extracts of various cell lines, using E2F-4 antibody (NBP3-15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/E2F-4-Antibody-3O6Y9-Western-Blot-NBP3-15850-img0001.jpg "Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850] - Western blot analysis of extracts of various cell lines, using E2F-4 antibody (NBP3-15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")