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![Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850] - Western blot analysis of extracts of various cell lines, using E2F-4 antibody (NBP3-15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/E2F-4-Antibody-3O6Y9-Western-Blot-NBP3-15850-img0001.jpg "Western Blot: E2F-4 Antibody (3O6Y9) [NBP3-15850] - Western blot analysis of extracts of various cell lines, using E2F-4 antibody (NBP3-15850) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: E2F4 Overexpression Lysate (Adult Normal) [NBL1-10085] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E2F4.](http://images.novusbio.com/fullsize/E2F4-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-10085-img0002.jpg "Western Blot: E2F4 Overexpression Lysate (Adult Normal) [NBL1-10085] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for E2F4.")
![Western Blot: E2F-4 Antibody (S09-7E6) [NBP3-19623] - Western blot detection of E2F-4 in K562 cell lysates using NBP3-19623 (1:1000 diluted). Predicted band size: 44kDa. Observed band size: 65kDa.](http://images.novusbio.com/fullsize/E2F-4-Antibody-S09-7E6-Western-Blot-NBP3-19623-img0001.jpg "Western Blot: E2F-4 Antibody (S09-7E6) [NBP3-19623] - Western blot detection of E2F-4 in K562 cell lysates using NBP3-19623 (1:1000 diluted). Predicted band size: 44kDa. Observed band size: 65kDa.")
![Western Blot: E2F-4 Antibody (PSH0-45) [NBP3-32284] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32284) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Raji cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 10 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32284) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32284_rabbit-e2f-4-mab-psh0-45-278202416331256.jpg "Western Blot: E2F-4 Antibody (PSH0-45) [NBP3-32284] - Western blot analysis of E2F-4 on different lysates with Rabbit anti-E2F-4 antibody (NBP3-32284) at 1/1,000 dilution. Lane 1: 293T cell lysate Lane 2: Raji cell lysate Lane 3: HeLa cell lysate Lane 4: Jurkat cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 44 kDa Observed band size: 44 kDa Exposure time: 10 minutes; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32284) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.")
