DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." alt="CTLA4 was detected in immersion fixed paraffin-embedded sections of human Appendix using Rabbit Anti-Human CTLA4, Monoclonal Antibody (Catalog # NBP3-20446 ) at 1:100 at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module)and Dewax and HIER Buffer H (pH 9; Epredia Catalog #TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the membrane. Protocol available in COMET™ Panel Builder." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20818 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-31143SS | |||
| Azide and BSA Free | NBP2-80683 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11966-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20735 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20809 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-20819 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-41000-0.025mg | |||
DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # 
![Western Blot: CTLA-4 Antibody (37B3D8.2F2) [NBP2-31143] - Analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on (C) partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml.](http://images.novusbio.com/fullsize/CTLA4-Antibody-37B3D8-2F2-Western-Blot-NBP2-31143-img0001.jpg "Western Blot: CTLA-4 Antibody (37B3D8.2F2) [NBP2-31143] - Analysis of CTLA-4 protein on (A) mouse Kidney lysate (B) human Kidney lysate and on (C) partial recombinant protein using CTLA-4 monoclonal antibody at a concentration of 2 ug/ml.")
![ELISA: Rat CTLA-4 ELISA Kit (Colorimetric) [NBP3-06777] - Samples were spiked with high concentrations of Rat CTLA-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06777_rat-ctla-4-elisa-kit-colorimetric-132202416102264.jpg "ELISA: Rat CTLA-4 ELISA Kit (Colorimetric) [NBP3-06777] - Samples were spiked with high concentrations of Rat CTLA-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Rat CTLA-4 ELISA Kit (Colorimetric) [NBP3-06777] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06777_rat-ctla-4-elisa-kit-colorimetric-432024856793.jpg)
![Immunohistochemistry: CTLA-4 Antibody (PS01-34) [NBP3-32230] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CTLA-4 antibody (NBP3-32230) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32230) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32230_rabbit-ctla-4-mab-ps01-34-298202414161879.jpg "Immunohistochemistry: CTLA-4 Antibody (PS01-34) [NBP3-32230] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CTLA-4 antibody (NBP3-32230) at 1/1,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32230) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![ELISA: CTLA-4 Antibody (9H10) - Chimeric [NBP3-11966] - Binding curves of the rabbit chimeric version of the anti-CTLA-4 antibody 9H10 (NBP3-11966; red line) and isotype control (anti-Fluorescein antibody NBP2-52638 4-4-20 (enhanced); blue line) to an ELISA plate coated with mouse CTLA-4-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using NBP3-11966. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used.](http://images.novusbio.com/fullsize/CTLA-4-Antibody-9H10-Chimeric-ELISA-NBP3-11966-img0001.jpg "ELISA: CTLA-4 Antibody (9H10) - Chimeric [NBP3-11966] - Binding curves of the rabbit chimeric version of the anti-CTLA-4 antibody 9H10 (NBP3-11966; red line) and isotype control (anti-Fluorescein antibody NBP2-52638 4-4-20 (enhanced); blue line) to an ELISA plate coated with mouse CTLA-4-Fc fusion protein at a concentration of 5 ug/ml. A 3-fold serial dilution from 10,000 to 0.17 ng/ml was performed using NBP3-11966. For signal detection, a 1:4000 dilution of HRP-labelled anti-rabbit IgG1 antibody was used.")
![ELISA: Mouse CTLA-4 ELISA Kit (Colorimetric) [NBP3-06829] - Samples were spiked with high concentrations of Mouse CTLA-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.](http://images.novusbio.com/fullsize/nbp3-06829_mouse-ctla-4-elisa-kit-colorimetric-132202416232312.jpg "ELISA: Mouse CTLA-4 ELISA Kit (Colorimetric) [NBP3-06829] - Samples were spiked with high concentrations of Mouse CTLA-4 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.")
![ELISA: Mouse CTLA-4 ELISA Kit (Colorimetric) [NBP3-06829] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-06829_mouse-ctla-4-elisa-kit-colorimetric-4320249202613.jpg)
![Immunohistochemistry-Paraffin: CTLA-4 Antibody (rCTLA4/7219) [NBP3-20431] - CTLA-4 was stained in human FFPE tonsil tissue. HIER antigen retrieval at pH 9 for 20min. Concentration used: 1:50. AF647 version of the antibody was used (Catalog # NBP3-20809AF647). Image from a verified customer review.](http://images.novusbio.com/fullsize/nbp3-20431_mouse-ctla-4-mab-rctla4-7219-1772024104462.jpg)
![Western Blot:CTLA-4 Overexpression Lysate[NBP2-07774]](http://images.novusbio.com/fullsize/nbp2-10649_p120-catenin-overexpression-lysate-western-blot-181202613311117.jpg "Western Blot:CTLA-4 Overexpression Lysate[NBP2-07774]")
![Western Blot:CTLA-4 Overexpression Lysate[NBP2-07774]](http://images.novusbio.com/fullsize/nbp2-07774_ctla-4-overexpression-lysate-western-blot-181202616104911.jpg)
