| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12027-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-54307-100ug | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90956-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12026-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF740 | |||
| Carrier Free | AF740-SP | |||
![Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric [NBP3-12027] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 16C10 (NBP3-12027) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12027, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/B7-1-CD80-Antibody-16C10-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12027-img0001.jpg "Immunocytochemistry/Immunofluorescence: B7-1/CD80 Antibody (16C10) - Chimeric [NBP3-12027] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of 16C10 (NBP3-12027) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12027, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.")
![Flow Cytometry: B7-1/CD80 Antibody (16C10) - Chimeric - Azide and BSA Free [NBP3-12027] - Daudi cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12027 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12027_rabbit-monoclonal-b7-1-cd80-antibody-16c10-chimeric-7420252211308.png)
![Western Blot: B7-1/CD80 Antibody (8-E5-R) [NBP3-32169] - Western blot analysis of B7-1/CD80 on human brain tissue lysates with Mouse anti-B7-1/CD80 antibody (NBP3-32169) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32169) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32169_mouse-b7-1-cd80-mab-8-e5-r-278202415295510.jpg "Western Blot: B7-1/CD80 Antibody (8-E5-R) [NBP3-32169] - Western blot analysis of B7-1/CD80 on human brain tissue lysates with Mouse anti-B7-1/CD80 antibody (NBP3-32169) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32169) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
