| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-12079-0.025mg | |||
MAB002) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-Associated Proteins protocol. " alt="Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CD74 Monoclonal Antibody (Catalog # MAB35901) or (B) Mouse IgG1 isotype control (MAB002) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-Associated Proteins protocol. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB35901-SP | |||
| Carrier Free | FAB35901V-100UG | |||
| Carrier Free | FAB35901U-100UG | |||
| Carrier Free | FAB35901T-100UG | |||
| Carrier Free | FAB35901S-100UG | |||
| Carrier Free | FAB35901R-100UG | |||
| Carrier Free | FAB35901N-100UG | |||
| Carrier Free | FAB35901G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-44989 | |||
| Azide and BSA Free | NBP2-34778-0.1mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-08927 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-50423-0.025ml | |||
MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." alt="A20 mouse B cell lymphoma cell line was stained with Rat Anti-Mouse CD74 Monoclonal Antibody (Catalog # MAB7478, filled histogram) or isotype control antibody (Catalog # MAB005, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB7478A | |||
| Carrier Free | MAB7478-SP | |||
| Carrier Free | FAB7478V-100UG | |||
| Carrier Free | FAB7478U-100UG | |||
| Carrier Free | FAB7478T-100UG | |||
| Carrier Free | FAB7478S-100UG | |||
| Carrier Free | FAB7478R-100UG | |||
| Carrier Free | FAB7478N-100UG | |||
| Carrier Free | FAB7478G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| IHC-Prediluted | NBP2-48027 | |||
| Azide and BSA Free | NBP3-11423 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24340 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-11486 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-34568-0.1mg | |||
![Western Blot: CD74 Antibody (LN-2) - Chimeric [NBP3-12079] - Daudi cell lysate (A), human spleen (B) and human tonsil (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of LN-2 (NBP3-12079) at 0.0001 ug/ml, 0.001 ug/ml and 0.0001 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Chimeric-Western-Blot-NBP3-12079-img0001.jpg "Western Blot: CD74 Antibody (LN-2) - Chimeric [NBP3-12079] - Daudi cell lysate (A), human spleen (B) and human tonsil (C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of LN-2 (NBP3-12079) at 0.0001 ug/ml, 0.001 ug/ml and 0.0001 ug/ml, respectively, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.")
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2) - Chimeric [NBP3-12079] - Immunofluorescence analysis of paraformaldehyde fixed Daudi cells on Shi-fix(TM) coverslips stained with the chimeric rabbit IgG version of LN-2 (NBP3-12079) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-12079, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-Chimeric-Immunocytochemistry-Immunofluorescence-NBP3-12079-img0002.jpg)
![Flow Cytometry: CD74 Antibody (LN-2) - Chimeric - Azide and BSA Free [NBP3-12079] - Paraformaldehyde fixed Daudi cells were stained with anti-unknown specificity antibody (isotype control, black line) or NBP3-12079 (blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.](http://images.novusbio.com/fullsize/nbp3-12079_rabbit-monoclonal-cd74-antibody-ln-2-chimeric-7420252226527.png)
MAB002,open histogram) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (![Immunocytochemistry/Immunofluorescence: CD74 Antibody (SPM523) [NBP2-32947] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (SPM523) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)](http://images.novusbio.com/fullsize/CD74-Antibody-SPM523-Immunocytochemistry-Immunofluorescence-NBP2-32947-img0003.jpg "Immunocytochemistry/Immunofluorescence: CD74 Antibody (SPM523) [NBP2-32947] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (SPM523) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red)")
![Immunohistochemistry-Paraffin: CD74 Antibody (SPM523) [NBP2-32947] - Formalin-paraffin human tonsil stained with CD74 Ab (SPM523).](http://images.novusbio.com/fullsize/CD74-Antibody-SPM523-Immunohistochemistry-Paraffin-NBP2-32947-img0002.jpg)
![Flow Cytometry: CD74 Antibody (SPM523) [NBP2-32947] - Flow Cytometric Analysis of Human Raji cells using CD74 Antibody (SPM523) followed by Goat anti-Mouse IgG-CF488 (Blue). Isotype Control (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-SPM523-Flow-Cytometry-NBP2-32947-img0004.jpg)
![Western Blot: CD74 Antibody (CLIP/3127R) [NBP3-07225] - Western Blot Analysis of Human 293T cell lysate using CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-3127R-Western-Blot-NBP3-07225-img0003.jpg "Western Blot: CD74 Antibody (CLIP/3127R) [NBP3-07225] - Western Blot Analysis of Human 293T cell lysate using CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).")
![Immunohistochemistry-Paraffin: CD74 Antibody (CLIP/3127R) [NBP3-07225] - Formalin-fixed, paraffin-embedded human Tonsil stained with CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R).](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-3127R-Immunohistochemistry-Paraffin-NBP3-07225-img0002.jpg)
![Flow Cytometry: CD74 Antibody (CLIP/3127R) [NBP3-07225] - Flow Cytometric Analysis of Raji cells.CD74 Recombinant Rabbit Monoclonal Antibody (CLIP/3127R) followed by goat anti- Mouse IgG-CF488 (Blue). Isotype Control (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-3127R-Flow-Cytometry-NBP3-07225-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2 + CLIP/813) [NBP2-44992] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2 + CLIP/813) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-+-CLIP-813-Immunocytochemistry-Immunofluorescence-NBP2-44992-img0002.jpg "Immunocytochemistry/Immunofluorescence: CD74 Antibody (LN-2 + CLIP/813) [NBP2-44992] - Immunofluorescence Analysis of Raji cells labeling CD74 with CD74 Antibody (LN-2 + CLIP/813) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).")
![Immunohistochemistry-Paraffin: CD74 Antibody (LN-2 + CLIP/813) [NBP2-44992] - Human Tonsil stained with CD74 Monoclonal Antibody (LN-2 + CLIP/813).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-+-CLIP-813-Immunohistochemistry-Paraffin-NBP2-44992-img0001.jpg)
![Flow Cytometry: CD74 Antibody (LN-2 + CLIP/813) [NBP2-44992] - Flow Cytometric Analysis of Raji cells. CD74 Antibody (LN-2 + CLIP/813) followed by goat anti-Mouse IgG-CF488. Orange: cells are not permeabilized. Blue: cells are permeabilized. Isotype Control (Red).](http://images.novusbio.com/fullsize/CD74-Antibody-LN-2-+-CLIP-813-Flow-Cytometry-NBP2-44992-img0003.jpg)
![Immunohistochemistry: CD74 Antibody (PDM0-22) [NBP3-32167] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Mouse anti-CD74 antibody (NBP3-32167) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32167) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-32167_mouse-cd74-mab-pdm0-22-298202414262622.jpg "Immunohistochemistry: CD74 Antibody (PDM0-22) [NBP3-32167] - Immunohistochemical analysis of paraffin-embedded human B-cell lymphoma tissue with Mouse anti-CD74 antibody (NBP3-32167) at 1/5,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32167) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunocytochemistry/ Immunofluorescence: CD74 Antibody (PDM0-22) [NBP3-32167] - Immunocytochemistry analysis of LOVO cells labeling CD74 with Mouse anti-CD74 antibody (NBP3-32167) at 1/50 dilution.<br/><br/> Cells were fixed in 4% paraformaldehyde for 30 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-CD74 antibody (NBP3-32167) at 1/50 dilution in 2% BSA overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.](http://images.novusbio.com/fullsize/nbp3-32167_mouse-cd74-mab-pdm0-22-292024111481.jpg)
![Western blot analysis on different lysates with NBP3-32167 at 1/1,000 dilution. Lane 1: Daudi cell lysate Lane 2: Raji cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 33 kDa Observed band size: 35 kDa Exposure time: 2 minutes; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. NBP3-32167 at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat anti-Mouse IgG [HRP] at 1:150,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32167_-western-blot-639204590074299696.jpg)
![Western Blot: CD74 Antibody (CLIP/1133) [NBP2-44991] - Analysis of Raji Cell Lysate using CD74 Monoclonal Antibody (CLIP/1133)](http://images.novusbio.com/fullsize/CD74-Antibody-CLIP-1133-Western-Blot-NBP2-44991-img0001.jpg "Western Blot: CD74 Antibody (CLIP/1133) [NBP2-44991] - Analysis of Raji Cell Lysate using CD74 Monoclonal Antibody (CLIP/1133)")
