CD74 Antibody (332516) [Alexa Fluor® 647]

Human PBMC lymphocytes were stained with (A) Mouse Anti-Human CD74 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB35901R) or (B) Mouse IgG1 isotype control (IC002R) and Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-Associated Proteins protocol.

K562 human myelogenous leukemia cell line was stained with Mouse Anti-Human CD74 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB35901R, filled histogram) or Mouse IgG1 isotype control (IC002R,open histogram). Staining was performed using our Staining Membrane-Associated Proteins protocol.

• Reactivity: Hu
• Applications: Flow
• Clone: 332516
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.

CD74 Antibody (332516) [Alexa Fluor® 647] Summary

• Immunogen: Chinese hamster ovary cell line CHO-S-derived recombinant human CD74
  Gln73-Met232
  Accession # NP_004346
• Specificity: Detects recombinant human CD74 in direct ELISAs.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: CD74
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.25-1 ug/10^6 cells
• Application Notes: Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells.

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: 0.09% Sodium Azide
• Concentration: Please see the vial label for concentration. If unlisted please contact technical services.
• Reconstitution Instructions: Reconstitution default not known

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CD74 Antibody (332516) [Alexa Fluor® 647]

• CD74 antigen (invariant polypeptide of major histocompatibility complex, classII antigen-associated)
• CD74 antigen
• CD74 molecule, major histocompatibility complex, class II invariant chain
• CD74
• CLIP
• DHLAG
• DHLAGgamma chain of class II antigens
• HLA class II histocompatibility antigen gamma chain
• HLADG
• HLA-DR antigens-associated invariant chain
• HLA-DR-gamma
• Ia antigen-associated invariant chain
• Ia-associated invariant chain
• Ia-gamma
• Ii
• INVG34
• MHC HLA-DR gamma chain
• p33

Background

CD74 is a transmembrane protein which, on antigen presenting cells, is the invariant chain that chaperones MHC class II dimers from the endoplasmic reticulum to the cell surface. The cytoplasmic portion of CD74 can be released by intramembrane cleavage and activate NF kappa B. CD74 is up-regulated in several cancers and is also expressed by non-immune cells during inflammation. This antibody was raised to a splice variant of human CD74 which lacks the Thyroglobulin type 1 repeat in the extracellular domain. Within comparable regions, human CD74 shares 70% and 73% amino acid sequence identity with mouse and rat CD74, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CD74 Antibody (FAB35901R)(1)

Publication using FAB35901R

• Ragnar O. Vilmundarson, Niloufar Heydarikhorneh, An Duong, Tiffany Ho, Kianoosh Keyhanian, Fariborz Soheili et al. Savior Siblings Might Rescue Fetal Lethality But Not Adult Lymphoma in Irf2bp2-Null Mice Frontiers in Immunology 2022-07-04 [PMID: 35865523] (Immunohistochemistry, Transgenic Mouse)

Bioinformatics

• Gene Symbol: CD74
• Uniprot:
  • NP_004346()