FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." alt="Human peripheral blood mononuclear cells (PBMCs) gated on CD3-cells were stained with Mouse Anti-Human NCAM-1/CD56 APC-conjugated Monoclonal Antibody (Catalog # FAB2408A) and either (A) Mouse Anti-Human DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB666) or (B) Mouse IgG1Isotype Control (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B)." class="big_thumb" />
666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DNAM-1/ CD226 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF666). The ND50 is typically 0.5-1.5 µg/mL." alt="Recombinant Human DNAM-1/CD226 Fc Chimera (Catalog # 666-DN), immobilized onto a microplate, supports the adhesion of the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line). Adhesion elicited by Recombinant Human DNAM-1/CD226 Fc Chimera (20 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human DNAM-1/ CD226 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF666). The ND50 is typically 0.5-1.5 µg/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF666-SP | |||
FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB4436) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." alt="Mouse splenocytes were stained with Rat Anti-Mouse CD8a APC-conjugated Monoclonal Antibody (Catalog # FAB116A) and either (A) Rat Anti-Mouse DNAM-1/CD226 Monoclonal Antibody (Catalog # MAB4436) or (B) Rat IgG2BIsotype Control (Catalog # MAB0061) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB4436-SP | |||
| Carrier Free | FAB4436V-100UG | |||
| Carrier Free | FAB4436U-100UG | |||
| Carrier Free | FAB4436T-100UG | |||
| Carrier Free | FAB4436S-100UG | |||
| Carrier Free | FAB4436R-100UG | |||
| Carrier Free | FAB4436N-100UG | |||
| Carrier Free | FAB4436G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85001-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF4436-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-12268-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-68690-25ul | |||
666-DN) to Biotinylated Recombinant Human CD155/PVR Fc Chimera Avi-tag (![Western Blot: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Whole cell lysate (10 ug) from SUP-M2, RT-4, HH, U-251MG, MJ, HDLM-2, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-DNAM-1/CD226 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-BLR063G-Western-Blot-NBP2-77357-img0008.jpg "Western Blot: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Whole cell lysate (10 ug) from SUP-M2, RT-4, HH, U-251MG, MJ, HDLM-2, Jurkat, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-DNAM-1/CD226 recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .")
![Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate: DAB.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-BLR063G-Immunocytochemistry-Immunofluorescence-NBP2-77357-img0013.jpg)
![Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-BLR063G-Immunohistochemistry-Paraffin-NBP2-77357-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Rabbit anti-DNAM-1 recombinant monoclonal antibody [BLR063G]. Secondary: HRPconjugated goat anti-rabbit IgG. Substrate:DAB.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-BLR063G-Immunocytochemistry-Immunofluorescence-NBP2-77357-img0012.jpg)
![<b>Independent Antibodies Validation. </b>Immunoprecipitation: DNAM-1/CD226 Antibody (BLR063G) [NBP2-77357] - Detection of human DNAM-1/CD226 by WB of immunoprecipitates from HH lysate. Antibodies: Rabbit anti-DNAM-1/CD226 recombinant monoclonal [BLR063G] (NBP2-77357) and another rabbit anti-DNAM-1/CD226 recombinant monoclonal [BL-799B-1B9].](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-BLR063G-Immunoprecipitation-NBP2-77357-img0005.jpg)
![Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody [NBP1-85001] - Staining of human small intestine shows positivity in lymphoid cells.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-Immunohistochemistry-Paraffin-NBP1-85001-img0009.jpg "Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody [NBP1-85001] - Staining of human small intestine shows positivity in lymphoid cells.")
![Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody [NBP1-85001] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-Immunohistochemistry-Paraffin-NBP1-85001-img0003.jpg)
![Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody [NBP1-85001] - Staining of human spleen shows moderate positivity in lymphoid cells.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-Immunohistochemistry-Paraffin-NBP1-85001-img0005.jpg)
![Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody [NBP1-85001] - Staining of human tonsil shows strong positivity in lymphoid cells.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-Immunohistochemistry-Paraffin-NBP1-85001-img0006.jpg)
![Western Blot: DNAM-1/CD226 Antibody [NBP2-32247] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD226 antibody NBP2-32247 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-Western-Blot-NBP2-32247-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: DNAM-1/CD226 Antibody [NBP2-32247] - Samples: Whole cell lysate (50 ug) prepared using NETN buffer from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified rabbit anti-CD226 antibody NBP2-32247 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: DNAM-1/CD226 Antibody [NBP2-32247] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN buffer from Jurkat cells. Antibodies: Affinity purified rabbit anti-CD226 antibody NBP2-32247 used for IP at 6 ug per reaction. CD226 was also immunoprecipitated by rabbit anti-CD226 antibody BL16224. For blotting immunoprecipitated CD226, NBP2-32247 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-Immunoprecipitation-NBP2-32247-img0002.jpg)
![Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 | Lane 2: RT4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil](http://images.novusbio.com/fullsize/nbp3-21236_rabbit-dnam-1-cd226-pab-4520231553132.jpg)
![Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody (MM0248-1X20) [NBP2-12268] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-12268. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-MM0248-1X20-Immunohistochemistry-Paraffin-NBP2-12268-img0001.jpg "<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: DNAM-1/CD226 Antibody (MM0248-1X20) [NBP2-12268] - 10% Buffer formalin fixed and parffine embedded human placental tissue section (4um) is subjected IHC staining using NBP2-12268. Tissue section was pretreated in citric buffer (ph6.0) with microwave for antige retrieval before IHC is applied.")
![Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody [NBP2-68690] - Staining of human cell line HEL shows localization to midbody. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/DNAM-1-CD226-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-68690-img0001.jpg "Immunocytochemistry/Immunofluorescence: DNAM-1/CD226 Antibody [NBP2-68690] - Staining of human cell line HEL shows localization to midbody. Antibody staining is shown in green.")
![Western Blot: CD226 Overexpression Lysate (Adult Normal) [NBL1-08922] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD226.](http://images.novusbio.com/fullsize/CD226-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-08922-img0002.jpg "Western Blot: CD226 Overexpression Lysate (Adult Normal) [NBL1-08922] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CD226.")
