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![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP1-84437] - Staining in human skin and skeletal muscle tissues using anti-CALML5 antibody. Corresponding CALML5 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP1-84437-img0008.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: CALML5 Antibody [NBP1-84437] - Staining in human skin and skeletal muscle tissues using anti-CALML5 antibody. Corresponding CALML5 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunocytochemistry/Immunofluorescence: CALML5 Antibody [NBP1-84437] - Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane & cytosol.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84437-img0006.jpg)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP1-84437] - Staining of human skin shows strong cytoplasmic and nuclear positivity in stratum granulosum of epidermis.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP1-84437-img0002.jpg)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP1-84437] - Staining of human skeletal muscle shows low expression as expected.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP1-84437-img0007.jpg)
![Western Blot: CALML5 Antibody (2F10) [H00051806-M16] - Analysis of CALML5 expression in transfected 293T cell line by CALML5 monoclonal antibody (M16), clone 2F10. Lane 1: CALML5 transfected lysatE (15.9 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize2/CALML5-Antibody-(2F10)-Western-Blot-H00051806-M16-img0004.jpg "Western Blot: CALML5 Antibody (2F10) [H00051806-M16] - Analysis of CALML5 expression in transfected 293T cell line by CALML5 monoclonal antibody (M16), clone 2F10. Lane 1: CALML5 transfected lysatE (15.9 KDa). Lane 2: Non-transfected lysate.")
![Immunohistochemistry-Paraffin: CALML5 Antibody (2F10) [H00051806-M16] - Analysis of monoclonal antibody to CALML5 on formalin-fixed paraffin-embedded human kidney. Antibody concentration 3 ug/ml](http://images.novusbio.com/fullsize2/CALML5-Antibody-(2F10)-Immunohistochemistry-Paraffin-H00051806-M16-img0002.jpg)
![Sandwich ELISA: CALML5 Antibody (2F10) [H00051806-M16] - Detection limit for recombinant GST tagged CALML5 is 0.03 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize2/CALML5-Antibody-(2F10)-Sandwich-ELISA-H00051806-M16-img0003.jpg)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human mammary adenocarcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP2-99872-img0003.jpg "Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human mammary adenocarcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.")
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human skin (from donor 1) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium. The left panel: tissue incubated with primary antibody](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP2-99872-img0001.jpg)
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-99872] - Immunochemical staining of human CALML5 in human skin (from donor 2) with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to epithelium.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP2-99872-img0002.jpg)
![Western Blot: CALML5 Antibody [NBP2-15668] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:1000.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Western-Blot-NBP2-15668-img0001.jpg "Western Blot: CALML5 Antibody [NBP2-15668] - Sample (30 ug of whole cell lysate) A: A431 15% SDS PAGE gel, diluted at 1:1000.")
![Immunohistochemistry-Paraffin: CALML5 Antibody [NBP2-15668] - Immunohistochemical analysis of paraffin-embedded hepatoma, using antibody at 1:500 dilution.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Immunohistochemistry-Paraffin-NBP2-15668-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: CALML5 Antibody (158) [NBP2-89982] - Staining of Human CALML5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CALML5 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).](http://images.novusbio.com/fullsize2/CALML5-Antibody-(158)-Immunocytochemistry-Immunofluorescence-NBP2-89982-img0001.jpg "Immunocytochemistry/Immunofluorescence: CALML5 Antibody (158) [NBP2-89982] - Staining of Human CALML5 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human CALML5 monoclonal antibody (1:60) at 4? overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).")
![Flow Cytometry: CALML5 Antibody (158) [NBP2-89982] - Analysis of Human CALML5 expression on A431 cells. The cells were treated, stained with purified anti-Human CALML5, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.](http://images.novusbio.com/fullsize2/CALML5-Antibody-(158)-Flow-Cytometry-NBP2-89982-img0002.jpg)
![Western Blot: CALML5 Antibody [NBP2-92815] - Analysis of extracts of various cell lines, using CALML5 .Exposure time: 90s.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Western-Blot-NBP2-92815-img0003.jpg "Western Blot: CALML5 Antibody [NBP2-92815] - Analysis of extracts of various cell lines, using CALML5 .Exposure time: 90s.")
![Western Blot: CALML5 Overexpression Lysate (Adult Normal) [NBL1-08653] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CALML5.](http://images.novusbio.com/fullsize/CALML5-Overexpression-Lysate-(Adult-Normal)-Western-Blot-NBL1-08653-img0002.jpg "Western Blot: CALML5 Overexpression Lysate (Adult Normal) [NBL1-08653] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CALML5.")
![Immunohistochemistry-Paraffin: CALML5 Antibody (119) [NBP2-89981] - Staining of human CALML5 in human skin with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize2/CALML5-Antibody-(119)-Immunohistochemistry-Paraffin-NBP2-89981-img0001.jpg "Immunohistochemistry-Paraffin: CALML5 Antibody (119) [NBP2-89981] - Staining of human CALML5 in human skin with rabbit monoclonal antibody (1:200).")
![Sandwich ELISA: CALML5 Antibody Pair [HRP] [NBP2-79365] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.](http://images.novusbio.com/fullsize2/CALML5-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79365-img0001.jpg "Sandwich ELISA: CALML5 Antibody Pair [HRP] [NBP2-79365] - This standard curve is only for demonstration purposes. A standard curve should be generated for each assay.")
![ELISA: Human CALML5 ELISA Kit (Colorimetric) [NBP2-80352] - Example standard curve for Human CALML5 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.](http://images.novusbio.com/fullsize2/Human-CALML5-ELISA-Kit-(Colorimetric)-ELISA-NBP2-80352-img0001.jpg "ELISA: Human CALML5 ELISA Kit (Colorimetric) [NBP2-80352] - Example standard curve for Human CALML5 ELISA Kit (Colorimetric). These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.")