HAF008). A specific band was detected for TL1A/TNFSF15 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_lightbox">

HAF008). A specific band was detected for TL1A/TNFSF15 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." alt="Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and human pancreas tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB74422) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (
HAF008). A specific band was detected for TL1A/TNFSF15 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 1." class="big_thumb" />
MAB1050) followed by Goat anti-Rabbit IgG PE-conjugated Secondary Antibody (
F0110) and Mouse anti-Human CCR9 APC-conjugated Monoclonal Antibody (
FAB1791A). Cells were gated on CD4+ lymphocytes using Mouse anti-Human CD4 FITC-conjugated Monoclonal Antibody (
FAB3791F). View our protocol for
Staining Membrane-associated Proteins." class="big_lightbox">

MAB1050) followed by Goat anti-Rabbit IgG PE-conjugated Secondary Antibody (
F0110) and Mouse anti-Human CCR9 APC-conjugated Monoclonal Antibody (
FAB1791A). Cells were gated on CD4+ lymphocytes using Mouse anti-Human CD4 FITC-conjugated Monoclonal Antibody (
FAB3791F). View our protocol for
Staining Membrane-associated Proteins." class="big_thumb" />
VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_lightbox">

VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (
CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in epithelial cells. View our protocol for
IHC Staining with VisUCyte HRP Polymer Detection Reagents." class="big_thumb" />
1319-TL) induces apoptosis in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (
AR002). Apoptosis elicited by Recombinant Human TL1A/TNFSF15 (80 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB74422). The ND
50 is typically 0.04‑0.2 µg/mL." class="big_lightbox">

1319-TL) induces apoptosis in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin (
AR002). Apoptosis elicited by Recombinant Human TL1A/TNFSF15 (80 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Human TL1A/TNFSF15 Monoclonal Antibody (Catalog # MAB74422). The ND
50 is typically 0.04‑0.2 µg/mL." class="big_thumb" />