| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89153-25ul | |||
HAF016). A specific band was detected for CHD1L at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of Jurkat human acute T cell leukemia cell line, DU145 human prostate carcinoma cell line, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human CHD1L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1L at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6959-SP | |||
![Western Blot: CHD1L Antibody [NBP1-89153] - Analysis using Anti-CHD1L antibody NBP1-89153 (A) shows similar pattern to independent antibody NBP1-89154 (B).](http://images.novusbio.com/fullsize/CHD1L-Antibody-Western-Blot-NBP1-89153-img0012.jpg "<b>Independent Antibodies Validation. </b>Western Blot: CHD1L Antibody [NBP1-89153] - Analysis using Anti-CHD1L antibody NBP1-89153 (A) shows similar pattern to independent antibody NBP1-89154 (B).")
![<b>Independent Antibodies Validation. </b>Immunohistochemistry-Paraffin: CHD1L Antibody [NBP1-89153] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.](http://images.novusbio.com/fullsize/CHD1L-Antibody-Immunohistochemistry-Paraffin-NBP1-89153-img0020.jpg)
![Immunohistochemistry-Paraffin: CHD1L Antibody [NBP1-89153] - Staining of human cerebral cortex shows strong nuclear positivity in neurons.](http://images.novusbio.com/fullsize/CHD1L-Antibody-Immunohistochemistry-Paraffin-NBP1-89153-img0017.jpg)
![Immunohistochemistry-Paraffin: CHD1L Antibody [NBP1-89153] - Staining of human skin shows strong nuclear positivity in squamous epithelial cells.](http://images.novusbio.com/fullsize/CHD1L-Antibody-Immunohistochemistry-Paraffin-NBP1-89153-img0019.jpg)
![Western Blot: CHD1L Antibody [NBP2-59305] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CHD1L antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/CHD1L-Antibody-Western-Blot-NBP2-59305-img0003.jpg "Western Blot: CHD1L Antibody [NBP2-59305] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CHD1L antibody diluted at 1:5000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Western Blot: CHD1L Antibody [NBP2-59305] - CHD1L antibody immunoprecipitates CHD1L protein in IP experiments. IP Sample: 293T whole cell lysate/extract A. 40 ug 293T whole cell lysate/extract B. Control with 2 ug of preimmune rabbit IgG C. Immunoprecipitation of CHD1L protein by 2 ug of CHD1L antibody 7.5% SDS-PAGE The immunoprecipitated CHD1L protein was detected by CHD1L antibody diluted at 1:1000.](http://images.novusbio.com/fullsize/CHD1L-Antibody-Western-Blot-NBP2-59305-img0001.jpg)
![Western Blot: CHD1L Antibody [NBP2-59305] - Sample (30 ug of whole cell lysate) A: HepG2 B: HepG2 nucleus 5% SDS PAGE. Used at a dilution of 1:3000](http://images.novusbio.com/fullsize/CHD1L-Antibody-Western-Blot-NBP2-59305-img0002.jpg)
![Western Blot:[NBP3-30668]-Detection of human CHD1L by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T and Jurkat cells. Antibodies: Affinity purified rabbit anti-CHD1L antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. CHD1L was also immunoprecipitated by rabbit anti-CHD1L antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes and 10 seconds.](http://images.novusbio.com/fullsize/nbp3-30668_rabbit-chd1l-pab-23120251225655.jpg "Western Blot:[NBP3-30668]-Detection of human CHD1L by western blot and immunoprecipitation. Samples: Whole cell lysate from HEK293T and Jurkat cells. Antibodies: Affinity purified rabbit anti-CHD1L antibody used for WB at 0. 04 µg/ml and 0. 4 µg/ml and used for IP at 6 µg/mg lysate. CHD1L was also immunoprecipitated by rabbit anti-CHD1L antibody , which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes and 10 seconds.")
![Western Blot: CHD1L Antibody [NBP1-57105] - Jurkat cell lysate, concentration 2.5 ug/ml.](http://images.novusbio.com/fullsize/CHD1L-Antibody-Western-Blot-NBP1-57105-img0002.jpg "Western Blot: CHD1L Antibody [NBP1-57105] - Jurkat cell lysate, concentration 2.5 ug/ml.")
![Western Blot: CHD1L Antibody (3C1O9) [NBP3-15541] - Western blot analysis of extracts of HepG2 cells, using CHD1L Rabbit mAb (NBP3-15541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](http://images.novusbio.com/fullsize/CHD1L-Antibody-3C1O9-Western-Blot-NBP3-15541-img0001.jpg "Western Blot: CHD1L Antibody (3C1O9) [NBP3-15541] - Western blot analysis of extracts of HepG2 cells, using CHD1L Rabbit mAb (NBP3-15541) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
![Western Blot: CHD1L Overexpression Lysate (Adult Normal) [NBP2-07889] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHD1L.](http://images.novusbio.com/fullsize/CHD1L-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-07889-img0001.jpg "Western Blot: CHD1L Overexpression Lysate (Adult Normal) [NBP2-07889] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CHD1L.")
