| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9572-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | Qk017-0500 | |||
| Conjugate | Catalog # | Availability | Size | Price |
|---|---|---|---|---|
| Alexa Fluor 488 | NBP2-12331AF488 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9571-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB9573-SP | |||
![N/A GDF-15 [HRP]](http://images.novusbio.com/fullsize/elisa/qk957_human-gdf-15-quantikine-quickit-elisa-198202092311.jpg "N/A GDF-15 [HRP]")
![N/A GDF-15 [HRP]](http://images.novusbio.com/fullsize/elisa/qk957_human-gdf-15-quantikine-quickit-elisa-19820209276.jpg)
![Western Blot: GDF-15 Antibody [NBP1-42732] - Analysis using Biotin conjugate of NBP1-42732. Detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions.](http://images.novusbio.com/fullsize/GDF-15-Antibody-Western-Blot-NBP1-42732-img0005.jpg "Western Blot: GDF-15 Antibody [NBP1-42732] - Analysis using Biotin conjugate of NBP1-42732. Detection of recombinant MBP-NAG-1 fusion protein (60 kDa) purified from E.coli (lane 1); yeast cell lysate expressing SUMO-mouse NAG-1 (42 kDa) (lane 2), and R&D human NAG-1 monomer purified from CHO-K1 cells (14 kDa) (lane 3). All lysates were run under reducing conditions.")
![Lane 1: Rec Ms GDF15 Protein Reduced [0.05ug]. Lane 2: Opal prestained Molecular Weight Marker. Lane 3: Rec Ms GDF15 Protein Non-Reduced [0.05ug]. Primary Antibody: GDF-15 antibody at 1.0µg/mL overnight at 2-8C. Secondary Antibody: Gt Anti-Rabbit [H&L] HRP at 1:40,000 for 30mins at RT. Blocking: Universal BlockOut Buffer at RT for 1hr. Predicted MW: 14kDa monomer and 28kDa dimer.](http://images.novusbio.com/fullsize/antibody/nbp1-42732_rabbit-polyclonal-gdf-15-antibody-western-blot-542023101057.jpg)
![Western Blot: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725] - Detection of recombinant GDF-15 protein present in Pichia pastoris whole cell lysates expressed in yeast cells : lane 1 - GDF-15 H variant with SUMO expression tag at 36 kDa; lane 2 - GDF-15 D variant with SUMO expression tag at 36 kDa; lane 3 - GDF-15 H variant; and lane 4 - GDF-15 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4C. For detection, a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the VersaDoc(TM) 4000MP Imaging System.](http://images.novusbio.com/fullsize/GDF-15-Antibody-7C12-B3-F2-Western-Blot-NBP1-42725-img0007.jpg "Western Blot: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725] - Detection of recombinant GDF-15 protein present in Pichia pastoris whole cell lysates expressed in yeast cells : lane 1 - GDF-15 H variant with SUMO expression tag at 36 kDa; lane 2 - GDF-15 D variant with SUMO expression tag at 36 kDa; lane 3 - GDF-15 H variant; and lane 4 - GDF-15 D variant. All lysates were run under reducing conditions. Primary antibody was used at a 1:1,000 dilution in TBS containing 1% BSA and 0.2% Tween, and reacted overnight at 4C. For detection, a 1:40,000 dilution of peroxidase conjugated Gt-a-Mouse IgG secondary antibody was used in Blocking Buffer for Fluorescent Western Blotting for 30 min at room temperature. Molecular weight estimation was made by comparison to prestained MW markers. Image was captured using the VersaDoc(TM) 4000MP Imaging System.")
![ELISA: GDF-15 Antibody (7C12.B3.F2) [NBP1-42725] - ELISA Results of GDF-15 antibody (7C12.B3.F2). Each well was coated in duplicate with 0.1ug of conjugate [H-variant Red line, D-variant Green line]. The working dilution is 1:35,000. The starting dilution of antibody was 5ug/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using HRP Conjugate Stabilizer, Rabbit Anti-Mouse IgG HRP conjugated and TMB substrate.](http://images.novusbio.com/fullsize/GDF-15-Antibody-7C12-B3-F2-ELISA-NBP1-42725-img0008.jpg)
