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![Western Blot: B7-1/CD80 Antibody (8-E5-R) [NBP3-32169] - Western blot analysis of B7-1/CD80 on human brain tissue lysates with Mouse anti-B7-1/CD80 antibody (NBP3-32169) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32169) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32169_mouse-b7-1-cd80-mab-8-e5-r-278202415295510.jpg "Western Blot: B7-1/CD80 Antibody (8-E5-R) [NBP3-32169] - Western blot analysis of B7-1/CD80 on human brain tissue lysates with Mouse anti-B7-1/CD80 antibody (NBP3-32169) at 1/1,000 dilution. Lysates/proteins at 40 ug/Lane. Predicted band size: 33 kDa Observed band size: 60 kDa Exposure time: 24 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32169) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
