| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-89743-25ul | |||
F0101B). Quadrant markers were set based on isotype control antibody (MAB003). Staining was performed using our Staining Membrane-Associated Proteins protocol. " alt="HEK293 human embryonic kidney cell line transfected with (A) Human GPRC5A or (B) irrelevant protein and eGFP, was stained with Mouse Anti-Human GPRC5A Monoclonal Antibody (Catalog # MAB5239) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (F0101B). Quadrant markers were set based on isotype control antibody (MAB003). Staining was performed using our Staining Membrane-Associated Proteins protocol. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5239-SP | |||
| Carrier Free | IC5239R-100UG | |||
| Carrier Free | IC5239N-100UG | |||
| Carrier Free | IC5239V-100UG | |||
| Carrier Free | IC5239G-100UG | |||
| Carrier Free | IC5239U-100UG | |||
| Carrier Free | IC5239T-100UG | |||
| Carrier Free | IC5239S-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP3-24315 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58188-25ul | |||
![Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & vesicles. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-89743-img0015.jpg "Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human cell line U-2 OS shows localization to nucleoli, plasma membrane & vesicles. Antibody staining is shown in green.")
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human lung shows moderate membranous positivity in pneumocytes.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0024.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human skeletal muscle shows no positivity in myocytes as expected.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0023.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human small intestine shows moderate positivity in apical membrane in glandular cells.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0026.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody [NBP1-89743] - Staining of human urinary bladder shows moderate membranous positivity in urothelial cells.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunohistochemistry-Paraffin-NBP1-89743-img0025.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - (J) RAI3 has a conspicuously low expression in BxPc3 and PaCaDD165 in comparison to the other cell lines in the group. (n = 3). (PLoS One. 2017; 12 (1): e0170390. Published online 2017 Jan 23.)](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0012.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Wester Blot: Pancreatic cell lines, where NC1 and NC2 represent negative controls.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-NBP1-89743-img0014.jpg)
![Western Blot: GPRC5A/RAI3 Antibody [NBP1-89743] - Influence of GPRC5a knockout on the proliferation & migration ability in MIA PaCa-2 & TB32047 cells. (A–D) Western blot & sequencing results showing the GPRC5a knocked out in MIA PaCa-2-KO1/2 & TB32047-KO1/2 (Red highlight area means the different part in comparison of sequencing sequence). (E,F): The knockout of GPRC5a inhibited the proliferation ability of MIA PaCa-2 & TB32047 cells. (G,H) GPRC5a knockout inhibited the migration ability of MIA PaCa-2 (G) & TB32047 cells (H). * p-value < 0.05. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29949874), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-89743_rabbit-polyclonal-gprc5a-rai3-antibody-31020241530422.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-212024939131.jpg "<b>Genetic Strategies Validation. </b>Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![<b>Genetic Strategies Validation. </b>Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - RAI3 antibody [HL1864] detects RAI3 protein at cell membrane by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. RAI3 stained by RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249432857.jpg)
![Western Blot: GPRC5A/RAI3 Antibody (HL1864) - Azide and BSA Free [NBP3-25620] - Non-transfected (-) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-2120249342722.jpg)
![<b>Orthogonal Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded human tissues.</br>GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251832019.jpg)
![<b>Genetic Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunofluorescent analysis.<br>Sample: Wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min.</br>Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.</br>Blue: Fluoroshield with DAPI](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518404315.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Boiled and unboiled wild-type (WT) and GPRC5A/RAI3 knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br><br>*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518412910.jpg)
![<b>Independent Antibodies Validation and Genetic Strategies Validation.</b>Non-transfected (-) and transfected (+) unboiled MCF-7 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membranes were blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:5000 and competitor's antibody (Competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.<br><br>*The competitor is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518442215.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518423013.jpg)
![GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein at cell membrane by immunofluorescent analysis.</br>Sample: MCF-7 cells were fixed in ice-cold MeOH for 5 min.</br>Green: GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:500.</br>Blue: Fluoroshield with DAPI .](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunocytochemistry-immunofluorescence-1411202518335919.jpg)
![<b>Independent Antibodies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded human kidney.</br>GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100 and highly cited competitor's antibody diluted at 1:100.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min</p>*Competitor's antibody is not affiliated withand does not endorse this product.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251838289.jpg)
![<b>Orthogonal Strategies Validation. </b>Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-1411202518404314.jpg)
![<b>Orthogonal Strategies Validation. </b>GPRC5A/RAI3 antibody [HL1864] detects GPRC5A/RAI3 protein by immunohistochemical analysis.</br>Sample: Paraffin-embedded human tissues.</br>GPRC5A/RAI3 stained by GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:100.</br>Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-immunohistochemistry-paraffin-141120251840545.jpg)
![<b>Expression of Tagged Proteins Validation. </b>Unboiled non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with GPRC5A/RAI3 antibody [HL1864] (NBP3-25620) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/nbp3-25620_rabbit-gprc5a-rai3-mab-hl1864-azide-and-bsa-free-western-blot-141120251838173.jpg)
MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2Secondary Antibody (Catalog # ![Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in human ovarian cancer.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-H00009052-B01P-img0001.jpg "Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in human ovarian cancer.")
![Western Blot: GPRC5A/RAI3 Antibody [H00009052-B01P] - Analysis of GPRC5A expression in transfected 293T cell line by GPRC5A polyclonal antibody. Lane 1: GPRC5A transfected lysate(39.27 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Western-Blot-H00009052-B01P-img0002.jpg)
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.](http://images.novusbio.com/fullsize/nbp3-23915_mouse-gprc5a-rai3-mab-gprc5a-7893-2412025863688.jpg "Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder carcinoma stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). Inset: PBS instead of primary antibody; secondary only negative control.")
![Immunohistochemistry-Paraffin: GPRC5A/RAI3 Antibody (GPRC5A/7893) [NBP3-23915] - Formalin-fixed, paraffin-embedded human bladder stained with GPRC5A/RAI3 Antibody (GPRC5A/7893). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.](http://images.novusbio.com/fullsize/nbp3-23915_mouse-gprc5a-rai3-mab-gprc5a-7893-2412025872358.jpg)
![Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP2-58188] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.](http://images.novusbio.com/fullsize/GPRC5A-RAI3-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58188-img0001.jpg "Immunocytochemistry/Immunofluorescence: GPRC5A/RAI3 Antibody [NBP2-58188] - Staining of human cell line U-2 OS shows localization to plasma membrane & vesicles.")
![Immunoprecipitation:[NBP3-30588]-Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated GPRC5A, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30588_rabbit-gprc5a-rai3-pab-231202511585558.jpg "Immunoprecipitation:[NBP3-30588]-Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit antibody used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit antibody. For blotting immunoprecipitated GPRC5A, was used at 0. 4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![Western Blot: RAI3 Overexpression Lysate (Adult Normal) [NBL1-11303] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAI3.](http://images.novusbio.com/fullsize/RAI3-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-11303-img0002.jpg "Western Blot: RAI3 Overexpression Lysate (Adult Normal) [NBL1-11303] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RAI3.")
![Immunoprecipitation:GPRC5A/RAI3 AntibodyNBP3-30195] -Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody NBP3-30195 (lot NBP3-30195-1) used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A.. For blotting immunoprecipitated GPRC5A, NBP3-30195 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/nbp3-30195_rabbit-gprc5a-rai3-pab-3012202416502223.jpg "Immunoprecipitation:GPRC5A/RAI3 AntibodyNBP3-30195] -Detection of human GPRC5A by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from HeLa cells. Antibodies: Affinity purified rabbit anti-GPRC5A antibody NBP3-30195 (lot NBP3-30195-1) used for IP at 6 µg per reaction. GPRC5A was also immunoprecipitated by rabbit anti-GPRC5A.. For blotting immunoprecipitated GPRC5A, NBP3-30195 was used at 0.4 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![ELISA: Human GPRC5A/RAI3 ELISA Kit (Colorimetric) [NBP3-31735] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31735_human-gprc5a-rai3-elisa-kit-colorimetric-206202415301538.png "ELISA: Human GPRC5A/RAI3 ELISA Kit (Colorimetric) [NBP3-31735] - Standard Curve Reference")
![ELISA: Human GPRC5A/RAI3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31736] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-31736_human-gprc5a-rai3-ready-to-use-elisa-kit-colorimetric-206202415314879.png "ELISA: Human GPRC5A/RAI3 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-31736] - Standard Curve Reference")
