HAF019). Specific bands were detected for Ccd1/DIXDC1 at approximately 59 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." alt="Western blot shows lysates of mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse Ccd1/DIXDC1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5599) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). Specific bands were detected for Ccd1/DIXDC1 at approximately 59 and 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF5599-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-12691-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-58141-25ul | |||
CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neopallial cortex and midbrain. View our protocol for ![Western Blot: Ccd1/DIXDC1 Antibody [NBP3-12691] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:77 kDa](http://images.novusbio.com/fullsize/Ccd1-DIXDC1-Antibody-Western-Blot-NBP3-12691-img0001.jpg "Western Blot: Ccd1/DIXDC1 Antibody [NBP3-12691] - Lane A: HeLa Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:77 kDa")
![Immunoprecipitation: Ccd1/DIXDC1 Antibody [NBP3-12691] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-DIXDC1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-DIXDC1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 77 kDaObserved band size :95 kDa](http://images.novusbio.com/fullsize/Ccd1-DIXDC1-Antibody-Immunoprecipitation-NBP3-12691-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: Ccd1/DIXDC1 Antibody [NBP3-12691] - Staining of DIXDC1 in U2OS cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-12691 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.](http://images.novusbio.com/fullsize/nbp3-12691_rabbit-polyclonal-ccd1-dixdc1-antibody-23520251664789.png)
![Western Blot: Ccd1/DIXDC1 Antibody [NBP2-58141] - Western blot analysis in control (vector only transfected HEK293T lysate) and DIXDC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).](http://images.novusbio.com/fullsize/Ccd1-DIXDC1-Antibody-Western-Blot-NBP2-58141-img0002.jpg "Western Blot: Ccd1/DIXDC1 Antibody [NBP2-58141] - Western blot analysis in control (vector only transfected HEK293T lysate) and DIXDC1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).")
![Immunocytochemistry/Immunofluorescence: Ccd1/DIXDC1 Antibody [NBP2-58141] - Staining of human cell line A-431 shows localization to cytosol.](http://images.novusbio.com/fullsize/Ccd1-DIXDC1-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-58141-img0001.jpg)
![Western Blot: Ccd1/DIXDC1 Antibody [NBP1-46147] - NBP1-46147 (0.5ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Ccd1-DIXDC1-Antibody-Western-Blot-NBP1-46147-img0002.jpg "Western Blot: Ccd1/DIXDC1 Antibody [NBP1-46147] - NBP1-46147 (0.5ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![SDS-Page: Recombinant Human Ccd1/DIXDC1 Protein [H00085458-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/qc_test-H00085458-P01-1.jpg "SDS-Page: Recombinant Human Ccd1/DIXDC1 Protein [H00085458-P01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Immunoprecipitation:[NBP3-29797]-Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies , and. For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29797_rabbit-ccd1-dixdc1-pab-23120251204044.jpg "Immunoprecipitation:[NBP3-29797]-Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies , and. For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Western Blot: DIXDC1 Overexpression Lysate (Adult Normal) [NBL1-09896] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DIXDC1.](http://images.novusbio.com/fullsize/DIXDC1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-09896-img0002.jpg "Western Blot: DIXDC1 Overexpression Lysate (Adult Normal) [NBL1-09896] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DIXDC1.")
![Western Blot: Ccd1/DIXDC1 Antibody [H00085458-B01P] - Analysis of DIXDC1 expression in transfected 293T cell line by DIXDC1 polyclonal antibody. Lane 1: DIXDC1 transfected lysate(51.92 KDa). Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Ccd1-DIXDC1-Antibody-Western-Blot-H00085458-B01P-img0002.jpg "Western Blot: Ccd1/DIXDC1 Antibody [H00085458-B01P] - Analysis of DIXDC1 expression in transfected 293T cell line by DIXDC1 polyclonal antibody. Lane 1: DIXDC1 transfected lysate(51.92 KDa). Lane 2: Non-transfected lysate.")
![Immunoprecipitation:[NBP3-29390]-Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies , and. For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-29390_rabbit-ccd1-dixdc1-pab-231202511585524.jpg "Immunoprecipitation:[NBP3-29390]-Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies , and. For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:Ccd1/DIXDC1 AntibodyNBP3-28983] -Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody NBP3-28983 (lot NBP3-28983-1) used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies, and . For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-28983_rabbit-ccd1-dixdc1-pab-3012202412392191.jpg "Immunoprecipitation:Ccd1/DIXDC1 AntibodyNBP3-28983] -Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody NBP3-28983 (lot NBP3-28983-1) used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies, and . For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
![Immunoprecipitation:[NBP3-30111]-Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies , and. For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/nbp3-30111_rabbit-ccd1-dixdc1-pab-231202511595060.jpg "Immunoprecipitation:[NBP3-30111]-Detection of human DIXDC1 by western blot of immunoprecipitates. Samples: Whole cell lysate from 293T cells. Antibodies: Affinity purified rabbit anti-DIXDC1 antibody used for IP at 6 µg/mg lysate. DIXDC1 was also immunoprecipitated by rabbit anti-DIXDC1 antibodies , and. For blotting immunoprecipitated DIXDC1, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.")
