Products Related to Gene-ID: 8200

CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox"> CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="GDF-5 was detected in immersion fixed frozen sections of mouse embyro (12 d.p.c.) using 15 µg/mL Goat Anti-Mouse GDF-5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF853) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />

HAF017). A specific band was detected for GDF‑5/BMP‑14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox"> HAF017). A specific band was detected for GDF‑5/BMP‑14 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

853-G5) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse GDF-5 (3 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF-5 Monoclonal Antibody (Catalog # MAB8531). The ND₅₀ is typically 2-10 µg/mL." class="big_lightbox"> 853-G5) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse GDF-5 (3 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF-5 Monoclonal Antibody (Catalog # MAB8531). The ND₅₀ is typically 2-10 µg/mL." alt="Recombinant Mouse GDF-5 (Catalog # 853-G5) induces alkaline phosphatase production in the ATDC5 mouse chondrogenic cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Mouse GDF-5 (3 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse GDF-5 Monoclonal Antibody (Catalog # MAB8531). The ND₅₀ is typically 2-10 µg/mL." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="GDF‑5/BMP‑14 was detected in immersion fixed U‑118 MG human glioblastoma/astrocytoma cells (positive) and absent in MCF-7 MG human glioblastoma cells (negative) using Mouse Anti-Human GDF‑5/BMP‑14 Monoclonal Antibody (Catalog # MAB8340) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />