| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1298-SP | |||
1299-NK) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human ULBP‑2 His-tag Avi-tag (AVI10538) that produces 50% of the optimal binding response is approximately 175-875 ng/mL." alt="When Recombinant Human NKG2D Fc Chimera Protein (1299-NK) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Biotinylated Recombinant Human ULBP‑2 His-tag Avi-tag (AVI10538) that produces 50% of the optimal binding response is approximately 175-875 ng/mL." class="big_thumb" />
1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP‑2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) binds with an ED50 of 0.500-8.00 ng/mL." alt="When Recombinant Human NKG2D/CD314 Fc Chimera Protein (1299-NK) is immobilized at 0.5 µg/mL (100 µL/well), Biotinylated Recombinant Human ULBP‑2 Fc Chimera Avi-tag Protein (Catalog # AVI1298) binds with an ED50 of 0.500-8.00 ng/mL." class="big_thumb" />
1299-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human ULBP-2 His-tag Protein (Catalog # 11196-UL) binds with an ED50 of 7.50-60.0 ng/mL." alt="When Recombinant Human NKG2D/CD314 Fc Chimera Protein (1299-NK) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human ULBP-2 His-tag Protein (Catalog # 11196-UL) binds with an ED50 of 7.50-60.0 ng/mL." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-94589-0.02ml | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-90060-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-94044-0.02ml | |||
![<b>Biological Strategies Validation. </b>Rh159 interferes with intracellular transport of NKG2DL.A) Association with Rh159 prevents intracellular transport of MICB. U373-MICB cells were transduced with adenovectors (MOI = 80) expressing either GFP (AdGFP) or FLAG-tagged Rh159 (AdRh159FL) under control of tetracycline-dependent transactivator provided by co-transduced AdtTA (MOI = 20). At 24 hpi cells were metabolically labeled for 30 min with [35S]cysteine + [35S]methionine. Upon chasing the label for the indicated times (h), cells were lysed and MICB was immunoprecipitated with anti–MICB mAb. Precipitates were either digested with EndoH (+) or mock treated (-) followed by SDS-PAGE and autoradiography. (S) indicates EndoH-deglycosylated proteins. B) Rh159 co-immunoprecipitates with MICB. U373-ULBP3 (ULBP3, left panel) or U373-MICB (MICB, right panel) cells were lysed at 48 h post-transduction with AdRh159FL (Rh159) or an adenovector expressing FLAG-tagged SVV ORF 61 (SVV61) used as a negative control. MICB and ULBP3 were immunoprecipitated with anti–MICB and anti-ULBP3 mouse and goat mAbs, respectively, then immunoblotted with mouse anti-FLAG mAb. The mouse IgG heavy chain (55kDa) is indicated (HC). Input lanes were loaded with 10% total lysate used in immunoprecipitation and immunoblotted with mAbs for FLAG and GAPDH. The results shown are representative of two independent experiments. C) Rh159 reduces steady state levels of MICB. U373-MICB cells were lysed at 48 h post-transduction with the indicated Ad-vectors. Lysates were digested with EndoH (+) or mock treated (-) then immunoblotted with mAbs for MICB, FLAG or GAPDH. Note that both MICB and Rh159 are EndoH sensitive consistent with ER localization. The results shown are representative of two independent experiments. D-E) Rh159 reduces surface expression of MICA, MICB, ULBP1 and ULBP2 but not ULBP3. U373-NKG2DL cells were transduced with AdRh159FL or AdGFP as in A) but for 48 h. Cells were then lysed and immunoblotted with mAbs for FLAG and GAPDH (D),](http://images.novusbio.com/fullsize/af1298_human-ulbp-2-5-6-affinity-purified-polyclonal-ab-8220249503219.jpg)
![<b>Biological Strategies Validation. </b>Rh159 interferes with intracellular transport of NKG2DL.A) Association with Rh159 prevents intracellular transport of MICB. U373-MICB cells were transduced with adenovectors (MOI = 80) expressing either GFP (AdGFP) or FLAG-tagged Rh159 (AdRh159FL) under control of tetracycline-dependent transactivator provided by co-transduced AdtTA (MOI = 20). At 24 hpi cells were metabolically labeled for 30 min with [35S]cysteine + [35S]methionine. Upon chasing the label for the indicated times (h), cells were lysed and MICB was immunoprecipitated with anti–MICB mAb. Precipitates were either digested with EndoH (+) or mock treated (-) followed by SDS-PAGE and autoradiography. (S) indicates EndoH-deglycosylated proteins. B) Rh159 co-immunoprecipitates with MICB. U373-ULBP3 (ULBP3, left panel) or U373-MICB (MICB, right panel) cells were lysed at 48 h post-transduction with AdRh159FL (Rh159) or an adenovector expressing FLAG-tagged SVV ORF 61 (SVV61) used as a negative control. MICB and ULBP3 were immunoprecipitated with anti–MICB and anti-ULBP3 mouse and goat mAbs, respectively, then immunoblotted with mouse anti-FLAG mAb. The mouse IgG heavy chain (55kDa) is indicated (HC). Input lanes were loaded with 10% total lysate used in immunoprecipitation and immunoblotted with mAbs for FLAG and GAPDH. The results shown are representative of two independent experiments. C) Rh159 reduces steady state levels of MICB. U373-MICB cells were lysed at 48 h post-transduction with the indicated Ad-vectors. Lysates were digested with EndoH (+) or mock treated (-) then immunoblotted with mAbs for MICB, FLAG or GAPDH. Note that both MICB and Rh159 are EndoH sensitive consistent with ER localization. The results shown are representative of two independent experiments. D-E) Rh159 reduces surface expression of MICA, MICB, ULBP1 and ULBP2 but not ULBP3. U373-NKG2DL cells were transduced with AdRh159FL or AdGFP as in A) but for 48 h. Cells were then lysed and immunoblotted with mAbs for FLAG and GAPDH (D),](http://images.novusbio.com/fullsize/af1298_human-ulbp-2-5-6-affinity-purified-polyclonal-ab-4420241542505.jpg)
![N/A ULBP-2 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_ULBP2_DY1298_ELISA_1620.jpg "N/A ULBP-2 [Biotin]")
AVI10538) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 30-38 kDa." class="big_thumb" />
![Immunocytochemistry/ Immunofluorescence: ULBP-2 Antibody - Azide and BSA Free [ULBP-2] - Immunofluorescence analysis of PC-12 cells using ULBP-2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](http://images.novusbio.com/fullsize/nbp2-94589_rabbit-polyclonal-ulbp-2-antibody-52202516283286.jpg "Immunocytochemistry/ Immunofluorescence: ULBP-2 Antibody - Azide and BSA Free [ULBP-2] - Immunofluorescence analysis of PC-12 cells using ULBP-2 Rabbit pAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human renal carcinoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ULBP-2-Antibody-022-Immunohistochemistry-Paraffin-NBP2-90060-img0003.jpg "Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human renal carcinoma with rabbit monoclonal antibody (1:200).")
![Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human gastric cancer with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ULBP-2-Antibody-022-Immunohistochemistry-Paraffin-NBP2-90060-img0004.jpg)
![Immunohistochemistry-Paraffin: ULBP-2 Antibody (022) [NBP2-90060] - Staining of human ULBP2 in human hepatoma with rabbit monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/ULBP-2-Antibody-022-Immunohistochemistry-Paraffin-NBP2-90060-img0002.jpg)
![Western Blot: ULBP2 Overexpression Lysate (Adult Normal) [NBL1-17606] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ULBP2.](http://images.novusbio.com/fullsize/ULBP2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-17606-img0002.jpg "Western Blot: ULBP2 Overexpression Lysate (Adult Normal) [NBL1-17606] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for ULBP2.")
![Western Blot: ULBP-2 Antibody - Azide and BSA Free [ULBP-2] - Western blot analysis of lysates from THP-1 cells, using ULBP-2 Rabbit pAb at 1:400 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit .<br>Exposure time: 180s.](http://images.novusbio.com/fullsize/nbp2-94044_rabbit-polyclonal-ulbp-2-antibody-5220251628045.jpg "Western Blot: ULBP-2 Antibody - Azide and BSA Free [ULBP-2] - Western blot analysis of lysates from THP-1 cells, using ULBP-2 Rabbit pAb at 1:400 dilution.<br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.<br>Lysates/proteins: 25ug per lane.<br>Blocking buffer: 3% nonfat dry milk in TBST.<br>Detection: ECL Enhanced Kit .<br>Exposure time: 180s.")
