| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF3709-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF6375-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-47774-0.1mg | |||
| IHC-Prediluted | NBP2-48224 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB7694-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-87000-25ul | |||
HAF007). A specific band was detected for ZAP70 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of CEM human T-lymphoblastoid cell line, MOLT-4 human acute lymphoblastic leukemia cell line, Jurkat human acute T cell leukemia cell line, and Raji human Burkitt's lymphoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human ZAP70 Monoclonal Antibody (Catalog # MAB3709) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZAP70 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB3709-SP | |||
![Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Immunocytochemistry-Immunofluorescence-NBP2-44502-img0006.jpg "Immunocytochemistry/Immunofluorescence: ZAP70 Antibody (2F3.2) [NBP2-44502] - Immunofluorescence Analysis of PFA-fixed Jurkat cells labeled with ZAP70 Antibody (2F3.2) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red).")
![Immunohistochemistry-Paraffin: ZAP70 Antibody (2F3.2) [NBP2-44502] - Human Tonsil stained with ZAP70 Monoclonal Antibody (2F3.2).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Immunohistochemistry-Paraffin-NBP2-44502-img0001.jpg)
![Flow Cytometry: ZAP70 Antibody (2F3.2) [NBP2-44502] - Flow Cytometric Analysis of PFA-fixed Jurkat cells. ZAP70 Antibody (2F3.2) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Flow-Cytometry-NBP2-44502-img0005.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Simple Western lane view shows a specific band for ZAP70 in 0.2 mg/ml of Jurkat (left) and H. Tonsil (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Simple-Western-NBP2-44502-img0002.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Electropherogram image of the corresponding Simple Western lane. ZAP70 antibody was used at 10 ug/ml dilution of Jurkat and H. Tonsil lysates(s) respectively.](http://images.novusbio.com/fullsize/ZAP70-Antibody-2F3-2-Simple-Western-NBP2-44502-img0003.jpg)
![Simple Western: ZAP70 Antibody (2F3.2) [NBP2-44502] - Lane view shows a specific band for ZAP70 in Jurkat cell lysate using 50 ug/mL antibody dilution. Electropherogram image of corresponding Simple Western lane view at WES molecular weight of 70 kDa. Image from an internal validation.](http://images.novusbio.com/fullsize/nbp2-44502_mouse-monoclonal-zap70-antibody-2f3-2-17720241003235.jpg)
![Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining in human lymph node and cerebral cortex tissues using anti-ZAP70 antibody. Corresponding ZAP70 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0007.jpg "<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining in human lymph node and cerebral cortex tissues using anti-ZAP70 antibody. Corresponding ZAP70 RNA-seq data are presented for the same tissues.")
![<b>Orthogonal Strategies Validation. </b>Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0006.jpg)
![Immunohistochemistry-Paraffin: ZAP70 Antibody [NBP1-87000] - Staining of human cerebral cortex shows low expression as expected.](http://images.novusbio.com/fullsize/ZAP70-Antibody-Immunohistochemistry-Paraffin-NBP1-87000-img0005.jpg)
![Lane 1: Marker [kDa] 230, 110, 82, 49, 32, 26, 18Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG spLane 4: Human plasma (IgG/HSA depleted)Lane 5: Human liver tissueLane 6: Human tonsil tissue](http://images.novusbio.com/fullsize/nbp1-87000_rabbit-polyclonal-zap70-antibody-304202513131169.jpg)
![Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0004.jpg "Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Mouse tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] detects ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) stained by ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Immunocytochemistry-Immunofluorescence-NBP3-13623-img0002.jpg)
![<b>Biological Strategies Validation. </b>Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Untreated (-) and treated (+) Jurkat whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0001.jpg)
![Western Blot: ZAP70 [p Tyr352] Antibody (GT1686) [NBP3-13623] - Rat tissue extract (50 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ZAP70 (phospho Tyr319) / Syk (phospho Tyr352) antibody [GT1686] (NBP3-13623) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (NBP2-19382) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/ZAP70-p-Tyr352-Antibody-GT1686-Western-Blot-NBP3-13623-img0003.jpg)
![Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Western-Blot-NBP2-89121-img0001.jpg "Western Blot: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 on Jurkat cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.")
![<b>Biological Strategies Validation. </b>Immunohistochemistry-Paraffin: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody( 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Immunohistochemistry-Paraffin-NBP2-89121-img0002.jpg)
![Flow Cytometry: ZAP70 Antibody (A1B5) [NBP2-89121] - Analysis of ZAP70 was done on Jurakt cells. The cells were fixed, permeabilized and stained with the primary antibody( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Mouse IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).](http://images.novusbio.com/fullsize/ZAP70-Antibody-A1B5-Flow-Cytometry-NBP2-89121-img0003.jpg)
![Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Ramos cell lysate (negative)<br/> Lane 3: Mouse spleen tissue lysate<br/> Lane 4: Mouse thymus tissue lysate<br/> Lane 5: Rat spleen tissue lysate<br/> Lane 6: Rat thymus tissue lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 70 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 1 minute 21 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-288202414191541.jpg "Western Blot: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/1,000 dilution.<br/><br/> Lane 1: Jurkat cell lysate<br/> Lane 2: Ramos cell lysate (negative)<br/> Lane 3: Mouse spleen tissue lysate<br/> Lane 4: Mouse thymus tissue lysate<br/> Lane 5: Rat spleen tissue lysate<br/> Lane 6: Rat thymus tissue lysate<br/><br/> Lysates/proteins at 20 ug/Lane.<br/><br/> Predicted band size: 70 kDa<br/> Observed band size: 70 kDa<br/><br/> Exposure time: 1 minute 21 seconds;<br/><br/> 4-20% SDS-PAGE gel.<br/><br/> Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33110) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Immunohistochemistry: ZAP70 Antibody (A1B5-R) [NBP3-33110] - Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-ZAP70 antibody (NBP3-33110) at 1/2,000 dilution.<br/><br/> The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-33110) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/nbp3-33110_mouse-zap70-mab-a1b5-r-29820241435257.jpg)
![Flow Cytometry: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10e6 cells stained with 1.0 mcg/ml Phospho Zap70 (Y319) antibody in a 150 mcl volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), antiphospho Zap70 (Y319) in H2O2 treated (blue).](http://images.novusbio.com/fullsize/ZAP70-[p-Tyr319]-Antibody-Flow-Cytometry-NBP1-18896-img0001.jpg "<b>Biological Strategies Validation. </b>Flow Cytometry: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Flow cytometric analysis of phospho-Zap70 (Y319). Jurkat cells were treated with H2O2 for 3 minutes. Cells were fixed in 1.5% PFA, permeabilized in 90%, and 1 X 10e6 cells stained with 1.0 mcg/ml Phospho Zap70 (Y319) antibody in a 150 mcl volume. Isotype anti-KLH control (black) in H2O2 treated cells, anti-phospho Zap70 (Y319) in untreated cells (red), antiphospho Zap70 (Y319) in H2O2 treated (blue).")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Whole cell lysate from Jurkat cells that had been treated with hydrogen peroxide. ZAP70 antibodies NBP1-18896, NBP1-1889, and NBP1-18898 used for IP at 3 mcg/mg lysate.](http://images.novusbio.com/fullsize/ZAP70-[p-Tyr319]-Antibody-Immunoprecipitation-NBP1-18896-img0004.jpg)
![<b>Biological Strategies Validation. </b>Immunoprecipitation: ZAP70 [p Tyr319] Antibody [NBP1-18896] - Jurkat WC Lysate treated with hydrogen peroxide (+) or mock treated (-). NB100-97826 was used to IP ZAP70. The IPs were either treated with phosphatase (+) or mock (-) treated.](http://images.novusbio.com/fullsize/ZAP70-[p-Tyr319]-Antibody-Immunoprecipitation-NBP1-18896-img0005.jpg)
