BDNF: Novus Biologicals

NBP2-67410

BDNF Antibody (SJ12-09)

(1 Publication)

100 ul

NBP2-67410

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse brain tissue using anti-BDNF antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: BDNF Antibody (SJ12-09) [NBP2-67410] - Analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.

Reactivity:

Hu, Mu, Rt, Ze

Species Glossary

Reactivity: Hu, Mu, Rt, Ze

Applications:

, ,

Applications: , ,

Host:

Rabbit Monoclonal

Host: Rabbit Monoclonal

Conjugate:

Unconjugated

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NB100-98683

BDNF Antibody - Azide and BSA Free

(1 Publication)

0.5 mg

NB100-98683

Immunohistochemistry: BDNF Antibody [NB100-98683] - Rat DRG using sheep antibody to neurofilament

Immunohistochemistry: BDNF Antibody [NB100-98683] - Rat RG at 40 ug/ml concentration using Rabbit antibody to mature BDNF (BDNF, Brain-derived neurotrophic factor, Abrineurin): IgG and DAPI counterstained

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NB100-98683-0.1mg

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11166-BD

Recombinant Human BDNF Protein, CF

(3 Publications)

10 μg

11166-BD

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED50 for this effect is 0.200-2.00 ng/mL.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	11166-BD-050
Carrier Free	11166-BD-250
Carrier Free	11166-BD-01M

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BT-BDNF-GMP

Recombinant Human BDNF GMP Protein, CF

25 μg

BT-BDNF-GMP

2 μg/lane of Recombinant Human BDNF GMP Protein (Catalog # BT-BDNF-GMP) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12 kDa, under reducing conditions.

Dopaminergic neurons were successfully differentiated from human iPSCs using Recombinant Human GMP BDNF (Catalog # BT-BDNF-GMP). Neurons show bright co-staining of beta tubulin 3 (TUJ1; green), tyrosine hydroxylase (TH; red), and DAPI (blue) to confirm dopaminergic neuron identity. BDNF successfully supported the derivation and maintenance of dopaminergic neurons.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	BT-BDNF-GMP-01M

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BT-BDNF

Recombinant Human BDNF Protein, CF

20 μg

BT-BDNF

BT-BDNF-GMP), Animal-Free (BT-BDNF-AFL) and RUO (Catalog # BT-BDNF) grades of Recombinant Human BDNF as measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB (orange, green, red, respectively)." class="big_lightbox">

Equivalent bioactivity of GMP (<a class=

BT-BDNF-GMP), Animal-Free (BT-BDNF-AFL) and RUO (Catalog # BT-BDNF) grades of Recombinant Human BDNF as measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB (orange, green, red, respectively)." alt="Equivalent bioactivity of GMP (BT-BDNF-GMP), Animal-Free (BT-BDNF-AFL) and RUO (Catalog # BT-BDNF) grades of Recombinant Human BDNF as measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB (orange, green, red, respectively)." class="big_thumb" />

Recombinant Human BDNF Protein (Catalog # BT-BDNF) stimulates cell proliferation of the BaF mouse pro-B cells transfected with TrkB. The ED<sub>50</sub> for this effect is 0.100-1.00 ng/mL.

2 μg/lane of Recombinant Human BDNF Protein (Catalog # BT-BDNF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12 kDa under reducing conditions.

BT-BDNF) was measured at a concentration range between 7.51 pM and 3846 pM. The double-referenced sensorgram was fit to a two-state reaction binding model to determine the binding kinetics and affinity, with an affinity constant of KD=21.3 pM. (Biacore T200)." class="big_lightbox">

Recombinant Human TrkB Fc Chimera was captured on a Sensor Chip Protein AGL, and binding to Recombinant Human BDNF GMP (<a class=

BT-BDNF) was measured at a concentration range between 7.51 pM and 3846 pM. The double-referenced sensorgram was fit to a two-state reaction binding model to determine the binding kinetics and affinity, with an affinity constant of KD=21.3 pM. (Biacore T200)." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	BT-BDNF-100
Carrier Free	BT-BDNF-250
Carrier Free	BT-BDNF-01M
Carrier Free	BT-BDNF-050

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NBP2-37276

BDNF Antibody (3D9C5) - BSA Free

0.1 ml

NBP2-37276

Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against human BDNF (AA: 19-248) recombinant protein. (Expected MW is 51.7 kDa)

Western Blot: BDNF Antibody (3D9C5) [NBP2-37276] - Analysis using BDNF mAb against HEK293 (1) and BDNF (AA: 19-248)-hIgGFc transfected HEK293 (2) cell lysate.

ELISA: BDNF Antibody (3D9C5) [NBP2-37276] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-37276-0.025ml

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MAB2481

BDNF Antibody (35909) [Unconjugated]

(1 Publication)

500 μg

MAB2481

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	IC2481U-100UG
Alexa Fluor 405	IC2481V-100UG
Alexa Fluor 488	IC2481G-100UG
Alexa Fluor 532	FAB2481X
Alexa Fluor 594	IC2481T-100UG
Alexa Fluor 647	IC2481R-100UG
Alexa Fluor 700	IC2481N-100UG
Alexa Fluor 750	IC2481S-100UG
Alexa Fluor Plus 405	FAB2481AFP405-100UG
Alexa Fluor Plus 488	FAB2481AFP488-100UG
Alexa Fluor Plus 555	FAB2481AFP555-100UG
Alexa Fluor Plus 594	FAB2481AFP594-100UG
Alexa Fluor Plus 647	FAB2481AFP647-100UG
Alexa Fluor Plus 680	FAB2481AFP680-100UG
Allophycocyanin	IC2481A
Biotin	FAB2481B
CoraFluor 1	FAB2481CL1
DyLight 350	FAB2481D
DyLight 405	FAB2481E
DyLight 488	FAB2481K
DyLight 550	FAB2481L
DyLight 594	FAB2481M
DyLight 650	FAB2481W
DyLight 680	FAB2481Y
DyLight 755	FAB2481Z
FITC	FAB2481F
HRP	FAB2481H
Janelia Fluor 525	FAB2481JF525
Janelia Fluor 549	FAB2481I
Janelia Fluor 646	FAB2481J
Janelia Fluor 669	FAB2481JF669
Phycoerythrin	IC2481P
mFluor Violet 450 SE	FAB2481MFV450
mFluor Violet 500 SE	FAB2481MFV500
mFluor Violet 610 SE	FAB2481MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC2481P
Carrier Free	IC2481A
Carrier Free	MAB2481-SP
Carrier Free	IC2481V-100UG
Carrier Free	IC2481U-100UG
Carrier Free	IC2481T-100UG
Carrier Free	IC2481S-100UG
Carrier Free	IC2481R-100UG
Carrier Free	IC2481N-100UG
Carrier Free	IC2481G-100UG

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MAB248R

BDNF Antibody (35928R) [Unconjugated]

100 μg

MAB248R

BDNF was detected in immersion fixed paraffin-embedded sections of human spinal cord using Mouse Anti-Human BDNF Monoclonal Antibody (Catalog # MAB248R) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB248RU-100UG
Alexa Fluor 405	FAB248RV-100UG
Alexa Fluor 488	FAB248RG-100UG
Alexa Fluor 532	FAB248RX-100UG
Alexa Fluor 594	FAB248RT-100UG
Alexa Fluor 647	FAB248RR-100UG
Alexa Fluor 700	FAB248RN-100UG
Alexa Fluor 750	FAB248RS-100UG
Alexa Fluor Plus 405	FAB248RAFP405-100UG
Alexa Fluor Plus 488	FAB248RAFP488-100UG
Alexa Fluor Plus 555	FAB248RAFP555-100UG
Alexa Fluor Plus 594	FAB248RAFP594-100UG
Alexa Fluor Plus 647	FAB248RAFP647-100UG
Alexa Fluor Plus 680	FAB248RAFP680-100UG
Allophycocyanin	FAB248RA
Biotin	FAB248RB
CoraFluor 1	FAB248RCL1
DyLight 350	FAB248RD
DyLight 405	FAB248RE
DyLight 488	FAB248RK
DyLight 550	FAB248RL
DyLight 594	FAB248RM
DyLight 650	FAB248RW
DyLight 680	FAB248RY
DyLight 755	FAB248RZ
FITC	FAB248RF
HRP	FAB248RH
Janelia Fluor 525	FAB248RJF525
Janelia Fluor 549	FAB248RI
Janelia Fluor 646	FAB248RJ
Janelia Fluor 669	FAB248RJF669
PE	FAB248RP
PerCP	FAB248RC
mFluor Violet 450 SE	FAB248RMFV450
mFluor Violet 500 SE	FAB248RMFV500
mFluor Violet 610 SE	FAB248RMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB248R-SP

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BT-BDNF-AFL

Recombinant Human BDNF Protein, Animal-Free

25 μg

BT-BDNF-AFL

BT-BDNF-GMP), Animal-Free (Catalog # BT-BDNF-AFL) and RUO (BT-BDNF) grades of Recombinant Human BDNF as measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB (orange, green, red, respectively)." class="big_lightbox">

BT-BDNF-GMP), Animal-Free (Catalog # BT-BDNF-AFL) and RUO (BT-BDNF) grades of Recombinant Human BDNF as measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB (orange, green, red, respectively)." alt="Equivalent bioactivity of GMP (BT-BDNF-GMP), Animal-Free (Catalog # BT-BDNF-AFL) and RUO (BT-BDNF) grades of Recombinant Human BDNF as measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB (orange, green, red, respectively)." class="big_thumb" />

Animal-Free™ Recombinant Human BDNF Protein (Catalog # BT-BDNF-AFL) stimulates cell proliferation of the BaF mouse pro-B cells transfected with TrkB. The ED<sub>50</sub> for this effect is 0.100-1.00 ng/mL.

2 μg/lane of Animal-Free™ Recombinant Human BDNF Protein (Catalog # BT-BDNF-AFL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 12 kDa.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	BT-BDNF-AFL-01M

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MAB3175

BDNF Antibody (584421) [Unconjugated] - Pro form

100 μg

MAB3175

BDNF was detected in immersion fixed paraffin-embedded sections of hippocampus using Mouse Anti-Human Pro BDNF Monoclonal Antibody (Catalog # mab3175) at 15 µg/ml overnight at 4 degrees Celsius followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB3175U-100UG
Alexa Fluor 405	FAB3175V-100UG
Alexa Fluor 488	FAB3175G-100UG
Alexa Fluor 532	FAB3175X-100UG
Alexa Fluor 594	FAB3175T-100UG
Alexa Fluor 647	FAB3175R-100UG
Alexa Fluor 700	FAB3175N-100UG
Alexa Fluor 750	FAB3175S-100UG
Alexa Fluor Plus 405	FAB3175AFP405-100UG
Alexa Fluor Plus 488	FAB3175AFP488-100UG
Alexa Fluor Plus 555	FAB3175AFP555-100UG
Alexa Fluor Plus 594	FAB3175AFP594-100UG
Alexa Fluor Plus 647	FAB3175AFP647-100UG
Alexa Fluor Plus 680	FAB3175AFP680-100UG
Allophycocyanin	FAB3175A
Biotin	FAB3175B
CoraFluor 1	FAB3175CL1
DyLight 350	FAB3175D
DyLight 405	FAB3175E
DyLight 488	FAB3175K
DyLight 550	FAB3175L
DyLight 594	FAB3175M
DyLight 650	FAB3175W
DyLight 680	FAB3175Y
DyLight 755	FAB3175Z
FITC	FAB3175F
HRP	FAB3175H
Janelia Fluor 525	FAB3175JF525
Janelia Fluor 549	FAB3175I
Janelia Fluor 646	FAB3175J
Janelia Fluor 669	FAB3175JF669
PE	FAB3175P
PerCP	FAB3175C
mFluor Violet 450 SE	FAB3175MFV450
mFluor Violet 500 SE	FAB3175MFV500
mFluor Violet 610 SE	FAB3175MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB3175-SP

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MAB848R

BDNF Antibody (37129R) [Unconjugated]

100 μg

MAB848R

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB848RU-100UG
Alexa Fluor 405	FAB848RV-100UG
Alexa Fluor 488	FAB848RG-100UG
Alexa Fluor 532	FAB848RX-100UG
Alexa Fluor 594	FAB848RT-100UG
Alexa Fluor 647	FAB848RR-100UG
Alexa Fluor 700	FAB848RN-100UG
Alexa Fluor 750	FAB848RS-100UG
Alexa Fluor Plus 405	FAB848RAFP405-100UG
Alexa Fluor Plus 488	FAB848RAFP488-100UG
Alexa Fluor Plus 555	FAB848RAFP555-100UG
Alexa Fluor Plus 594	FAB848RAFP594-100UG
Alexa Fluor Plus 647	FAB848RAFP647-100UG
Alexa Fluor Plus 680	FAB848RAFP680-100UG
Allophycocyanin	FAB848RA
Biotin	FAB848RB
CoraFluor 1	FAB848RCL1
DyLight 350	FAB848RD
DyLight 405	FAB848RE
DyLight 488	FAB848RK
DyLight 550	FAB848RL
DyLight 594	FAB848RM
DyLight 650	FAB848RW
DyLight 680	FAB848RY
DyLight 755	FAB848RZ
FITC	FAB848RF
HRP	FAB848RH
Janelia Fluor 525	FAB848RJF525
Janelia Fluor 549	FAB848RI
Janelia Fluor 646	FAB848RJ
Janelia Fluor 669	FAB848RJF669
PE	FAB848RP
PerCP	FAB848RC
mFluor Violet 450 SE	FAB848RMFV450
mFluor Violet 500 SE	FAB848RMFV500
mFluor Violet 610 SE	FAB848RMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB848R-SP

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NB100-98754

proBDNF Antibody - Azide and BSA Free

(1 Publication)

0.1 mg

NB100-98754

Reactivity:

Mu, Rt

Species Glossary

Reactivity: Mu, Rt

Applications:

, ,

Applications: , ,

Host:

Sheep Polyclonal

Host: Sheep Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Protein Or Peptide

Research Areas

Has Sample Size

Promotional Item

Species Glossary

Products Related to Gene-ID: 627

BDNF Antibody (SJ12-09)

BDNF Antibody - Azide and BSA Free

Recombinant Human BDNF Protein, CF

Recombinant Human BDNF GMP Protein, CF

Recombinant Human BDNF Protein, CF

BDNF Antibody (3D9C5) - BSA Free

BDNF Antibody (35909) [Unconjugated]

BDNF Antibody (35928R) [Unconjugated]

Recombinant Human BDNF Protein, Animal-Free

BDNF Antibody (584421) [Unconjugated] - Pro form

BDNF Antibody (37129R) [Unconjugated]

proBDNF Antibody - Azide and BSA Free