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![Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0002.jpg "<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Ser380] Antibody [NBP3-13243] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Ser380) antibody (NBP3-13243) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Ser380]-Antibody-Western-Blot-NBP3-13243-img0001.jpg)
![Immunoprecipitation: RSK1 [p Ser380] Antibody (1E11) [NBP3-26395] - Immunoprecipitating RSK1 [p Ser380] Antibody in Hela whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26395 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26395 (3ug) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20ug.)](http://images.novusbio.com/fullsize/nbp3-26395_rabbit-rsk1-p-ser-380-mab-1e11-26220241322153.jpg "Immunoprecipitation: RSK1 [p Ser380] Antibody (1E11) [NBP3-26395] - Immunoprecipitating RSK1 [p Ser380] Antibody in Hela whole cell lysate. Lane 1: Rabbit control IgG (1ug) instead of NBP3-26395 in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000). Lane 2: NBP3-26395 (3ug) + Hela whole cell lysate (1mg). Lane 3: Hela whole cell lysate (20ug.)")
![Western Blot: RSK1 [p Ser380] Antibody (1E11) [NBP3-26395] - Positive Western Blot detected in A549 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate. All lanes: RSK1 [p Ser380] Antibody at 1.75ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 90 KDa Observed band size: 90 KDa](http://images.novusbio.com/fullsize/nbp3-26395_rabbit-rsk1-p-ser-380-mab-1e11-28220241135165.jpg)
![Western Blot: RSK1 [p Ser380] Antibody (S05-7F3) [NBP3-20065] - Western blot detection of RSK1 (phospho-Ser380) in CHO-K1 cell lysates using NBP3-20065 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.](http://images.novusbio.com/fullsize/RSK1-p-Ser380-Antibody-S05-7F3-Western-Blot-NBP3-20065-img0001.jpg "Western Blot: RSK1 [p Ser380] Antibody (S05-7F3) [NBP3-20065] - Western blot detection of RSK1 (phospho-Ser380) in CHO-K1 cell lysates using NBP3-20065 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 83kDa.")
![Western Blot: RSK1 [p Thr359, p Ser363] Antibody (S01-5B1) [NBP3-20023] - Western blot detection of RSK1 in C6 cell lysates using NBP3-20023 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 90kDa.](http://images.novusbio.com/fullsize/RSK1-p-Thr359-p-Ser363-Antibody-S01-5B1-Western-Blot-NBP3-20023-img0001.jpg "Western Blot: RSK1 [p Thr359, p Ser363] Antibody (S01-5B1) [NBP3-20023] - Western blot detection of RSK1 in C6 cell lysates using NBP3-20023 (1:1000 diluted). Predicted band size: 83kDa. Observed band size: 90kDa.")
![Western Blot: RSK1 [p Thr573] Antibody (JE50-12) [NBP3-32798] - Western blot analysis of RSK1 on different lysates with Rabbit anti-RSK1 antibody (NBP3-32798) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: K-562 treated with 500nM PMA for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 200nM PMA for 4 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32798) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32798_rabbit-rsk1-p-thr573-mab-je50-12-288202412442953.jpg "Western Blot: RSK1 [p Thr573] Antibody (JE50-12) [NBP3-32798] - Western blot analysis of RSK1 on different lysates with Rabbit anti-RSK1 antibody (NBP3-32798) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: K-562 treated with 500nM PMA for 15 minutes cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa treated with 200nM PMA for 4 hours cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 83 kDa Observed band size: 83 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32798) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: RSK1 [p Thr573] Antibody [NBP3-13271] - Untreated (-) and treated (+) A431 whole cell extracts (15 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Thr573) antibody (NBP3-13271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Thr573]-Antibody-Western-Blot-NBP3-13271-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Thr573] Antibody [NBP3-13271] - Untreated (-) and treated (+) A431 whole cell extracts (15 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Thr573) antibody (NBP3-13271) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: RSK1 [p Thr359] Antibody [NBP3-13253] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Thr359) antibody (NBP3-13253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize/RSK1-[p-Thr359]-Antibody-Western-Blot-NBP3-13253-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: RSK1 [p Thr359] Antibody [NBP3-13253] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RSK1 (phospho Thr359) antibody (NBP3-13253) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.")
![Western Blot: RSK1 Overexpression Lysate (Adult Normal) [NBL1-15570] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RSK1.](http://images.novusbio.com/fullsize/RSK1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-15570-img0002.jpg "Western Blot: RSK1 Overexpression Lysate (Adult Normal) [NBL1-15570] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for RSK1.")
![ELISA: Human RSK1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40390] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40390_human-rsk1-ready-to-use-elisa-kit-colorimetric-2110202415282638.png "ELISA: Human RSK1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-40390] - Standard Curve Reference")
![ELISA: Human RSK1 ELISA Kit (Colorimetric) [NBP3-40389] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-40389_human-rsk1-elisa-kit-colorimetric-2110202415302949.png "ELISA: Human RSK1 ELISA Kit (Colorimetric) [NBP3-40389] - Standard Curve Reference")