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Products Related to Gene-ID: 5595

50 Results for "5595"
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NBP2-22203

ERK1 Antibody (1E5)

(1 Publication)
0.1 ml
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Hu, Mu, Rt, Pm
Species Glossary
Reactivity: Hu, Mu, Rt, Pm
WB, ELISA, Flow, ICC/IF, IHC
Applications: WB, ELISA, Flow, ICC/IF, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
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AF1018

ERK1/ERK2 [p Thr185, p Tyr187, p Thr202, p Tyr204] Antibody [Unconjugated]

(1 Review)
(43 Publications)
100 μg
AF1018
Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) was detected in immersion fixed paraffin-embedded sections of human Cortex using Rabbit Anti-Human Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) , Polyclonal Antibody (Catalog #AF1018) at 25ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ Plus 647 Goat anti-Rabbit IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RB) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and nucleus of the neuron. Protocol available in COMET™ Panel Builder.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, Flow, IHC, CyTOF-ready, mIF
Applications: WB, Simple Western, Flow, IHC, CyTOF-ready, mIF
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
         
Validated by:          
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1018-SP
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AF1576

ERK1/ERK2 Antibody [Unconjugated]

(1 Review)
(34 Publications)
100 μg
AF1576
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, C2C12 mouse myoblast cell line myoblast cell line, and C6 rat glioma cell line. PVDF membrane was probed with 0.2 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1/ERK2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1576) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western
Applications: WB, Simple Western
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
         
Validated by:          
Conjugate Catalog # Availability Size Price
Allophycocyanin AF1576A
Biotin AF1576B
CoraFluor 1 AF1576CL1
DyLight 350 AF1576D
DyLight 405 AF1576E
DyLight 488 AF1576K
DyLight 550 AF1576L
DyLight 594 AF1576M
DyLight 650 AF1576W
DyLight 680 AF1576Y
DyLight 755 AF1576Z
FITC AF1576F
HRP AF1576H
Janelia Fluor 525 AF1576JF525
Janelia Fluor 549 AF1576I
Janelia Fluor 646 AF1576J
Janelia Fluor 669 AF1576JF669
PE AF1576P
PerCP AF1576C
mFluor Violet 450 SE AF1576MFV450
mFluor Violet 500 SE AF1576MFV500
mFluor Violet 610 SE AF1576MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1576-SP
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DYC1018B-2

Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) DuoSet IC, 2 Plate

(33 Publications)
1 Kit (for 2 Plates)
DYC1018B-2
ELISA ERK1/ERK2 [p Thr185, p Thr202, p Tyr187, p Tyr204] [Biotin]
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1018BE
Biotin DYC1018B-5
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free DYC1018BE
Carrier Free DYC1018B-5
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MAB1018

ERK1/ERK2 [p Tyr187, p Tyr204, p Thr185, p Thr202] Antibody (269434) [Unconjugated]

(21 Publications)
100 μg
MAB1018
Western blot shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Rabbit Anti-Human PDGF (Catalog # 120-HD) and PC-12 rat adrenal pheochromocytoma cell line untreated or treated with 100 ng/mL Recombinant Rat beta -NGF (Catalog # 556-NG) and HeLa human cervical epithelial carcinoma cell line untreated or treated with 200 nM PMA. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat Phospho-ERK1 (T202/Y204)/ERK2 (T185/Y187) Monoclonal Antibody (MAB1018), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for Phospho-ERK1 (T202/Y204)/ ERK2 (T185/Y187) at approximately 40-45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, Simple Western, CyTOF-ready, Flow, ICC/IF
Applications: WB, Simple Western, CyTOF-ready, Flow, ICC/IF
Rabbit Monoclonal
Host: Rabbit Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 IC1018U-100UG
Alexa Fluor 405 IC1018V-100UG
Alexa Fluor 488 IC7806G-100UG
Alexa Fluor 532 FAB1018X
Alexa Fluor 594 IC1018T-100UG
Alexa Fluor 647 IC1018R-100UG
Alexa Fluor 700 IC1018N-100UG
Alexa Fluor 750 IC1018S-100UG
Allophycocyanin FAB1018A
Biotin FAB1018B
CoraFluor 1 FAB1018CL1
DyLight 350 FAB1018D
DyLight 405 FAB1018E
DyLight 488 FAB1018K
DyLight 550 FAB1018L
DyLight 594 FAB1018M
DyLight 650 FAB1018W
DyLight 680 FAB1018Y
DyLight 755 FAB1018Z
FITC FAB1018F
HRP FAB1018H
Janelia Fluor 525 FAB1018JF525
Janelia Fluor 549 FAB1018I
Janelia Fluor 646 FAB1018J
Janelia Fluor 669 FAB1018JF669
PE FAB1018P
PerCP FAB1018C
mFluor Violet 450 SE FAB1018MFV450
mFluor Violet 500 SE FAB1018MFV500
mFluor Violet 610 SE FAB1018MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free IC7806G-100UG
Carrier Free MAB1018-SP
Carrier Free IC1018V-100UG
Carrier Free IC1018U-100UG
Carrier Free IC1018T-100UG
Carrier Free IC1018S-100UG
Carrier Free IC1018R-100UG
Carrier Free IC1018N-100UG
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MAB1576

ERK1/ERK2 Antibody (216703) [Unconjugated]

(3 Reviews)
(20 Publications)
100 μg
MAB1576
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 0.5 µg/mL Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB1576) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ERK1 and ERK2 (2 ng/lane) were included. A specific band for ERK1/ERK2 was detected at approximately 44/42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 4.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC
Applications: WB, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Allophycocyanin FAB1576A
Biotin FAB1576B
CoraFluor 1 FAB1576CL1
DyLight 350 FAB1576D
DyLight 405 FAB1576E
DyLight 488 FAB1576K
DyLight 550 FAB1576L
DyLight 594 FAB1576M
DyLight 650 FAB1576W
DyLight 680 FAB1576Y
DyLight 755 FAB1576Z
FITC FAB1576F
HRP FAB1576H
Janelia Fluor 525 FAB1576JF525
Janelia Fluor 549 FAB1576I
Janelia Fluor 646 FAB1576J
Janelia Fluor 669 FAB1576JF669
PE FAB1576P
PerCP FAB1576C
mFluor Violet 450 SE FAB1576MFV450
mFluor Violet 500 SE FAB1576MFV500
mFluor Violet 610 SE FAB1576MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB1576-SP
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DYC1825-2

Phospho-ERK1 (T202/Y204) DuoSet IC ELISA, 2 Plate

(12 Publications)
1 Kit (for 2 Plates)
DYC1825-2
ELISA ERK1 [p Thr202, p Tyr204] [Biotin]
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
ELISA
Applications: ELISA
Conjugate Catalog # Availability Size Price
Biotin DYC1825E
Biotin DYC1825-5
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MAB15761

ERK1/ERK2 Antibody (631122) [Unconjugated]

(1 Review)
(3 Publications)
100 μg
MAB15761
Western blot shows lysates of MCF-7 human breast cancer cell line, BaF3 mouse pro-B cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat ERK1/ERK2 Monoclonal Antibody (Catalog # MAB15761) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for ERK1/ERK2 at approximately 42 and 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB
Applications: WB
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Allophycocyanin FAB15761A
Biotin FAB15761B
CoraFluor 1 FAB15761CL1
DyLight 350 FAB15761D
DyLight 405 FAB15761E
DyLight 488 FAB15761K
DyLight 550 FAB15761L
DyLight 594 FAB15761M
DyLight 650 FAB15761W
DyLight 680 FAB15761Y
DyLight 755 FAB15761Z
FITC FAB15761F
HRP FAB15761H
Janelia Fluor 525 FAB15761JF525
Janelia Fluor 549 FAB15761I
Janelia Fluor 646 FAB15761J
Janelia Fluor 669 FAB15761JF669
PE FAB15761P
PerCP FAB15761C
mFluor Violet 450 SE FAB15761MFV450
mFluor Violet 500 SE FAB15761MFV500
mFluor Violet 610 SE FAB15761MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB15761-SP
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AF1575

ERK1 Antibody [Unconjugated]

(2 Reviews)
(1 Publication)
50 μg
AF1575
Western blot shows lysates of MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, Jurkat human acute T cell leukemia cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.1 µg/mL Rabbit Anti-Human/Mouse/Rat ERK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1575) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). For additional reference, Recombinant Human Active ERK1 (Catalog # 1879-KS) and Recombinant Human Active ERK2 (Catalog # 1230-KS) (2 ng/lane) were included. A specific band for ERK1 was detected at approximately 44 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, IHC
Applications: WB, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF1575-SP
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NB110-96887

ERK1/2 Antibody - BSA Free

(2 Publications)
0.1 ml
NB110-96887
Western Blot: ERK1/2 Antibody [NB110-96887] - Western blot analysis of Human Cell line lysates showing detection of ERK1/2 protein using Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887). Load: 15 ugprotein. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Rabbit Anti-ERK1/2 Polyclonal Antibody (NB110-96887) at 1:1000 for 2 hours at RT. Secondary Antibody: Donkey Anti-Rabbit IgG: HRP for 1 hour at RT.
Hu, Mu, Rt, Bv, Ch, Dr, Sh, Xp
Species Glossary
Reactivity: Hu, Mu, Rt, Bv, Ch, Dr, Sh, Xp
WB, Flow, ICC/IF, IHC
Applications: WB, Flow, ICC/IF, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NB110-96887AF350
Alexa Fluor 405 NB110-96887AF405
Alexa Fluor 488 NB110-96887AF488
Alexa Fluor 532 NB110-96887AF532
Alexa Fluor 594 NB110-96887AF594
Alexa Fluor 647 NB110-96887AF647
Alexa Fluor 700 NB110-96887AF700
Alexa Fluor 750 NB110-96887AF750
Allophycocyanin NB110-96887APC
Biotin NB110-96887B
CoraFluor 1 NB110-96887CL1
DyLight 350 NB110-96887UV
DyLight 405 NB110-96887V
DyLight 488 NB110-96887G
DyLight 550 NB110-96887R
DyLight 594 NB110-96887DL594
DyLight 650 NB110-96887C
DyLight 680 NB110-96887FR
DyLight 755 NB110-96887IR
FITC NB110-96887F
HRP NB110-96887H
Janelia Fluor 525 NB110-96887JF525
Janelia Fluor 549 NB110-96887JF549
Janelia Fluor 646 NB110-96887JF646
Janelia Fluor 669 NB110-96887JF669
PE NB110-96887PE
PerCP NB110-96887PCP
mFluor Violet 450 SE NB110-96887MFV450
mFluor Violet 500 SE NB110-96887MFV500
mFluor Violet 610 SE NB110-96887MFV610
Available Formulations
Formulation Catalog # Availability Price  
BSA Free NB110-96887-0.025ml
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NBP2-45814

ERK1 Antibody (OTI4D7)

0.1 ml
NBP2-45814
Western Blot: ERK1 Antibody (OTI4D7) [NBP2-45814] - Analysis of extracts (35ug) from 9 different cell lines. (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Hu, Mu, Rt, Ca, Pm
Species Glossary
Reactivity: Hu, Mu, Rt, Ca, Pm
WB, ICC/IF, IHC
Applications: WB, ICC/IF, IHC
Mouse monoclonal
Host: Mouse monoclonal
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-71239AF350
Alexa Fluor 405 NBP2-71239AF405
Alexa Fluor 488 NBP2-71239AF488
Alexa Fluor 532 NBP2-71239AF532
Alexa Fluor 594 NBP2-71239AF594
Alexa Fluor 647 NBP2-71239AF647
Alexa Fluor 700 NBP2-71239AF700
Alexa Fluor 750 NBP2-71239AF750
Allophycocyanin NBP2-71239APC
Biotin NBP2-71239B
CoraFluor 1 NBP2-71239CL1
DyLight 350 NBP2-71239UV
DyLight 405 NBP2-71239V
DyLight 488 NBP2-71239G
DyLight 550 NBP2-71239R
DyLight 594 NBP2-71239DL594
DyLight 650 NBP2-71239C
DyLight 680 NBP2-71239FR
DyLight 755 NBP2-71239IR
FITC NBP2-71239F
HRP NBP2-71239H
Janelia Fluor 525 NBP2-71239JF525
Janelia Fluor 549 NBP2-71239JF549
Janelia Fluor 646 NBP2-71239JF646
Janelia Fluor 669 NBP2-71239JF669
PE NBP2-71239PE
PerCP NBP2-71239PCP
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NBP2-71239
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NBP2-71239

ERK1 Antibody (OTI4D7) - Azide and BSA Free

100 μg
NBP2-71239
Western Blot: ERK1 Antibody (OTI4D7) - Azide and BSA Free [NBP2-71239] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ERK1.
Hu, Mu, Rt, Ca, Pm
Species Glossary
Reactivity: Hu, Mu, Rt, Ca, Pm
WB, ICC/IF, IHC
Applications: WB, ICC/IF, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-71239AF350
Alexa Fluor 405 NBP2-71239AF405
Alexa Fluor 488 NBP2-71239AF488
Alexa Fluor 532 NBP2-71239AF532
Alexa Fluor 594 NBP2-71239AF594
Alexa Fluor 647 NBP2-71239AF647
Alexa Fluor 700 NBP2-71239AF700
Alexa Fluor 750 NBP2-71239AF750
Allophycocyanin NBP2-71239APC
Biotin NBP2-71239B
CoraFluor 1 NBP2-71239CL1
DyLight 350 NBP2-71239UV
DyLight 405 NBP2-71239V
DyLight 488 NBP2-71239G
DyLight 550 NBP2-71239R
DyLight 594 NBP2-71239DL594
DyLight 650 NBP2-71239C
DyLight 680 NBP2-71239FR
DyLight 755 NBP2-71239IR
FITC NBP2-71239F
HRP NBP2-71239H
Janelia Fluor 525 NBP2-71239JF525
Janelia Fluor 549 NBP2-71239JF549
Janelia Fluor 646 NBP2-71239JF646
Janelia Fluor 669 NBP2-71239JF669
PE NBP2-71239PE
PerCP NBP2-71239PCP
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