| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-56159SS | |||
HAF007). A specific band was detected for PKN1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and L1.2 mouse pro-B cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse PKN1 Monoclonal Antibody (Catalog # MAB6100) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6100-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85301-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-98761-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-95113-0.02ml | |||
![Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.Lane 1: PKN1 transfected lysate(103.9 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/PKN1-Antibody-1B10-Western-Blot-H00005585-M01-img0009.jpg "Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 expression in transfected 293T cell line by PKN1 monoclonal antibody (M01), clone 1B10.Lane 1: PKN1 transfected lysate(103.9 KDa).Lane 2: Non-transfected lysate.")
![Immunocytochemistry/Immunofluorescence: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of monoclonal antibody to PKN1 on HeLa cell . Antibody concentration 10 ug/ml.](http://images.novusbio.com/fullsize/PKN1-Antibody-1B10-Immunocytochemistry-Immunofluorescence-H00005585-M01-img0005.jpg)
![Western Blot: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 over-expressed 293 cell line, cotransfected with PKN1 Validated Chimera RNAi ( Cat # H00005585-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PKN1 monoclonal antibody (M01) clone 1B10 (Cat # H00005585-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.](http://images.novusbio.com/fullsize/PKN1-Antibody-1B10-Western-Blot-H00005585-M01-img0007.jpg)
![Immunoprecipitation: PKN1 Antibody (1B10) [H00005585-M01] - Analysis of PKN1 transfected lysate using anti-PKN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PKN1 monoclonal antibody.](http://images.novusbio.com/fullsize/PKN1-Antibody-1B10-Immunoprecipitation-H00005585-M01-img0006.jpg)
![ELISA: PKN1 Antibody (1B10) [H00005585-M01] - Detection limit for recombinant GST tagged PKN1 is approximately 3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PKN1-Antibody-1B10-ELISA-H00005585-M01-img0010.jpg)
![Western Blot: PKN1 Antibody [NBP1-85301] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/PKN1-Antibody-Western-Blot-NBP1-85301-img0006.jpg "Western Blot: PKN1 Antibody [NBP1-85301] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: PKN1 Antibody [NBP1-85301] - Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.](http://images.novusbio.com/fullsize/PKN1-Antibody-Immunohistochemistry-Paraffin-NBP1-85301-img0007.jpg)
![Western Blot: PKN1 Antibody [NBP1-85301] - Analysis in human cell line SCLC-21H.](http://images.novusbio.com/fullsize/PKN1-Antibody-Western-Blot-NBP1-85301-img0005.jpg)
![Western Blot: PKN1 Antibody [NBP2-98761] - Lane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:125 kDa](http://images.novusbio.com/fullsize/antibody/nbp2-98761_rabbit-polyclonal-pkn1-antibody-western-blot-229202394441..jpg "Western Blot: PKN1 Antibody [NBP2-98761] - Lane A: K562 Whole Cell LysateLane B: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:104 kDaObserved band size:125 kDa")
![Immunocytochemistry/Immunofluorescence: PKN1 Antibody [NBP2-98761] - Immunofluorescence staining of PKN1 in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with NBP2-98761 antibody (dilution ratio 1:300) at 4C overnight. Then cells were stained with the Alexa Fluor(R)488-conjugated Goat Anti-rabbit IgG Secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.](http://images.novusbio.com/fullsize/nbp2-98761_rabbit-polyclonal-pkn1-antibody-23520251125447.png)
![Western Blot: PKN1 Antibody (S09-2I4) [NBP3-19741] - Western blot detection of PKN1 in C6 cell lysates using NBP3-19741 (1:1000 diluted). Predicted band size: 104KDa. Observed band size: 120KDa.](http://images.novusbio.com/fullsize/PKN1-Antibody-S09-2I4-Western-Blot-NBP3-19741-img0001.jpg "Western Blot: PKN1 Antibody (S09-2I4) [NBP3-19741] - Western blot detection of PKN1 in C6 cell lysates using NBP3-19741 (1:1000 diluted). Predicted band size: 104KDa. Observed band size: 120KDa.")
![Western Blot: PKN1 Antibody [NBP2-95113] - Analysis of extracts of various cell lines, using PKN1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.](http://images.novusbio.com/fullsize/PKN1-Antibody-Western-Blot-NBP2-95113-img0004.jpg "Western Blot: PKN1 Antibody [NBP2-95113] - Analysis of extracts of various cell lines, using PKN1 at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit . Exposure time: 5s.")
![Western Blot: PKN1 Overexpression Lysate (Adult Normal) [NBP2-08073] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKN1.](http://images.novusbio.com/fullsize/PKN1-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-08073-img0001.jpg "Western Blot: PKN1 Overexpression Lysate (Adult Normal) [NBP2-08073] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for PKN1.")
![ELISA: Human PKN1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39046] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39046_human-pkn1-ready-to-use-elisa-kit-colorimetric-2110202415362367.png "ELISA: Human PKN1 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39046] - Standard Curve Reference")
![ELISA: Human PKN1 ELISA Kit (Colorimetric) [NBP3-39045] - Standard Curve Reference](http://images.novusbio.com/fullsize/nbp3-39045_human-pkn1-elisa-kit-colorimetric-211020241528248.png "ELISA: Human PKN1 ELISA Kit (Colorimetric) [NBP3-39045] - Standard Curve Reference")
