HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog #
4268-KS), Recombinant Human Active PKA C beta (Catalog #
4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_lightbox">
HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog #
4268-KS), Recombinant Human Active PKA C beta (Catalog #
4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat PKA C Pan Specific Monoclonal Antibody (Catalog # MAB4175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog #
HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog #
4268-KS), Recombinant Human Active PKA C beta (Catalog #
4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using
Immunoblot Buffer Group 3." class="big_thumb" />
HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." alt="Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Human/Mouse/Rat PKA C Pan Specific Monoclonal Antibody (Catalog # MAB4175) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (5 ng/lane) were included. A specific band for PKA C (pan) was detected at approximately 39 - 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3." class="big_thumb" />
![Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Western-Blot-NBP2-99335-img0004.jpg "Western Blot: PKA C alpha Antibody [NBP2-99335] - Anti-PKA C alpha rabbit polyclonal antibody at 1:500 dilution. Lane A: HeLa Whole Cell Lysate Lane B: MCF-7 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 40 kDa. Observed band size: 44 kDa")
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human testis with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0003.jpg)
![Immunohistochemistry-Paraffin: PKA C alpha Antibody [NBP2-99335] - Immunochemical staining of human PKA C alpha in human colon carcinoma with rabbit polyclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunohistochemistry-Paraffin-NBP2-99335-img0002.jpg)
![Western Blot: PKA C alpha Antibody [NBP2-57042] - Analysis in human cell line RT-4 and human cell line U-251 MG.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Western-Blot-NBP2-57042-img0002.jpg "Western Blot: PKA C alpha Antibody [NBP2-57042] - Analysis in human cell line RT-4 and human cell line U-251 MG.")
![Immunocytochemistry/Immunofluorescence: PKA C alpha Antibody [NBP2-57042] - Staining of human cell line MCF7 shows localization to cytosol & cytokinetic bridge. Antibody staining is shown in green.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-57042-img0001.jpg)
![Western Blot: PKA C alpha Antibody (7H3A4) [NBP2-37246] - Western blot analysis using PRKACA mAb against HEK293 (1) and PRKACA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-7H3A4-Western-Blot-NBP2-37246-img0003.jpg "Western Blot: PKA C alpha Antibody (7H3A4) [NBP2-37246] - Western blot analysis using PRKACA mAb against HEK293 (1) and PRKACA (AA: 1-120)-hIgGFc transfected HEK293 (2) cell lysate.")
![Western Blot: PKA C alpha Antibody (7H3A4) [NBP2-37246] - Western blot analysis using PRKACA mAb against human PRKACA (AA: 1-120) recombinant protein. (Expected MW is 39.7 kDa)](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-7H3A4-Western-Blot-NBP2-37246-img0002.jpg)
![ELISA: PKA C alpha Antibody (7H3A4) [NBP2-37246] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-7H3A4-ELISA-NBP2-37246-img0001.jpg)
![Proximity Ligation Assay: PKA C alpha Antibody (1D7) [H00005566-M02] - Analysis of protein-protein interactions between MAP3K3 and PRKACA. HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-PRKACA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-1D7-Proximity-Ligation-Assay-H00005566-M02-img0002.jpg "Proximity Ligation Assay: PKA C alpha Antibody (1D7) [H00005566-M02] - Analysis of protein-protein interactions between MAP3K3 and PRKACA. HeLa cells were stained with anti-MAP3K3 rabbit purified polyclonal 1:1200 and anti-PRKACA mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).")
![Sandwich ELISA: PKA C alpha Antibody (1D7) [H00005566-M02] - Detection limit for recombinant GST tagged PRKACA is approximately 0.1ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-1D7-Sandwich-ELISA-H00005566-M02-img0003.jpg)
![Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788] - Western blot analysis of PKA C (pan) on different lysates with Rabbit anti-PKA C (pan) antibody (NBP3-32788) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C6 cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32788) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/nbp3-32788_rabbit-pka-c-pan-p-thr197-mab-je45-17-288202412461616.jpg "Western Blot: PKA C (pan) [p Thr197] Antibody (JE45-17) - (Catalytic Subunit) [NBP3-32788] - Western blot analysis of PKA C (pan) on different lysates with Rabbit anti-PKA C (pan) antibody (NBP3-32788) at 1/1,000 dilution. Lane 1: NIH/3T3 cell lysate Lane 2: C6 cell lysate Lane 3: HeLa cell lysate Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate Lysates/proteins at 20 ug/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 46 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32788) at 1/1,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.")
![Western Blot: cAMP Protein Kinase Catalytic subunit alpha Overexpression Lysate (Adult Normal) [NBP2-04478] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for cAMP Protein Kinase Catalytic subunit alpha.](http://images.novusbio.com/fullsize/cAMP-Protein-Kinase-Catalytic-subunit-alpha-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-04478-img0001.jpg "Western Blot: cAMP Protein Kinase Catalytic subunit alpha Overexpression Lysate (Adult Normal) [NBP2-04478] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for cAMP Protein Kinase Catalytic subunit alpha.")
![Dot Blot: PKA C alpha Antibody (6D2.1) [NBP2-62195] - analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. NBP1-48359).The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1: GST-Akt1Lane 2: GST-Akt2Lane 3: GST-Akt3Lane 4: GST-PKAc alphaLane 5: GST-PKAc betaLane 6: GST-PKAc gammaLane 7: GST-MEK 1Lane 8: GST](http://images.novusbio.com/fullsize/PKA-C-alpha-Antibody-6D2-1-Dot-Blot-NBP2-62195-img0002.jpg "Dot Blot: PKA C alpha Antibody (6D2.1) [NBP2-62195] - analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05; cat. no. NBP1-48359).The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1: GST-Akt1Lane 2: GST-Akt2Lane 3: GST-Akt3Lane 4: GST-PKAc alphaLane 5: GST-PKAc betaLane 6: GST-PKAc gammaLane 7: GST-MEK 1Lane 8: GST")
